Effect of 60 minutes of renal ischemia followed by 10 and 90 minutes of reperfusion in rats: A possible role of oxygen free radicals

N. A. Vinodini, Y. Tripathi, C. V. Raghuveer

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Abstract

Objective: To evaluate the effect of reperfusion following renal ischemia in rat model. Methods: Seven animals in each group underwent 60 minutes of unilateral renal ischemia followed by reperfusion for 10 minutes and 90 minutes, the levels of biochemical parameters such, as malonylaldehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) were estimated after reperfusion serum urea was estimated apart from MDA, GSH and SOD. Results: A significant increase in the levels of MDA and a decrease in the tissue levels of GSH, SOD, and was observed when compared to that of experimental control group of animals. There was significant change in the serum urea level between the control and experimental groups. Results of the present study suggests that unilateral renal ischemia followed by reperfusion causes renal injury by increasing the levels of lipid peroxidation and the decreasing the levels of endogenous enzymes such as GSH and SOD. The pattern of result was similar in both the 10 minutes and 90 minutes of reperfusion groups suggesting that these levels were not related to duration of reperfusion. Conclusion: Therefore, the present study was designed to evaluate the effects of unilateral renal ischemia and reperfusion in a time dependent manner to show the involvement of oxygen free radicals in reperfusion injury.

Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalJournal of Chinese Clinical Medicine
Volume4
Issue number3
Publication statusPublished - 08-06-2009

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All Science Journal Classification (ASJC) codes

  • Medicine(all)

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