Effect of Amalaki rasayana on DNA damage and repair in randomized aged human individuals

Udupi Vishwanatha, Kanive P. Guruprasad, Puthiya M. Gopinath, Raviraj V. Acharya, Bokkasa V. Prasanna, Jayakrishna Nayak, Rajeshwari Ganesh, Jayalaxmi Rao, Rashmi Shree, Suchitra Anchan, Kothanahalli S. Raghu, Manjunath B. Joshi, Puspendu Paladhi, Panniampilly M. Varier, Kollath Muraleedharan, Thrikovil S. Muraleedharan, Kapaettu Satyamoorthy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ethnopharmacological relevance Preparations from Phyllanthus emblica called Amalaki rasayana is used in the Indian traditional medicinal system of Ayurveda for healthy living in elderly. The biological effects and its mechanisms are not fully understood. Since the diminishing DNA repair is the hallmark of ageing, we tested the influence of Amalaki rasayana on recognized DNA repair activities in healthy aged individuals. Methods Amalaki rasayana was prepared fresh and healthy aged randomized human volunteers were administrated with either rasayana or placebo for 45 days strictly as per the traditional text. The DNA repair was analyzed in peripheral blood mononuclear cells before and after rasayana administration and after 45 days post-rasayana treatment regimen. UVC-induced DNA strand break repair (DSBR) based on extent of DNA unwinding by fluorometric analysis, nucleotide excision repair (NER) by flow cytometry and constitutive base excision repair (BER) by gap filling method were analyzed. Results Amalaki rasayana administration stably maintained/enhanced the DSBR in aged individuals. There were no adverse side effects. Further, subjects with different body mass index showed differential DNA strand break repair capacity. No change in unscheduled DNA synthesis during NER and BER was observed between the groups. Conclusion Intake of Amalaki rasayana by aged individuals showed stable maintenance of DNA strand break repair without toxic effects. However, there was no change in nucleotide and base excision repair activities. Results warrant further studies on the effects of Amalaki rasayana on DSBR activities.

Original languageEnglish
Pages (from-to)387-397
Number of pages11
JournalJournal of Ethnopharmacology
Volume191
DOIs
Publication statusPublished - 15-09-2016

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DNA Repair
DNA Damage
DNA Breaks
Phyllanthus emblica
Amalakirasayana
Fluorometry
Poisons
DNA
Volunteers
Blood Cells
Flow Cytometry
Body Mass Index
Placebos
Maintenance

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

Vishwanatha, Udupi ; Guruprasad, Kanive P. ; Gopinath, Puthiya M. ; Acharya, Raviraj V. ; Prasanna, Bokkasa V. ; Nayak, Jayakrishna ; Ganesh, Rajeshwari ; Rao, Jayalaxmi ; Shree, Rashmi ; Anchan, Suchitra ; Raghu, Kothanahalli S. ; Joshi, Manjunath B. ; Paladhi, Puspendu ; Varier, Panniampilly M. ; Muraleedharan, Kollath ; Muraleedharan, Thrikovil S. ; Satyamoorthy, Kapaettu. / Effect of Amalaki rasayana on DNA damage and repair in randomized aged human individuals. In: Journal of Ethnopharmacology. 2016 ; Vol. 191. pp. 387-397.
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abstract = "Ethnopharmacological relevance Preparations from Phyllanthus emblica called Amalaki rasayana is used in the Indian traditional medicinal system of Ayurveda for healthy living in elderly. The biological effects and its mechanisms are not fully understood. Since the diminishing DNA repair is the hallmark of ageing, we tested the influence of Amalaki rasayana on recognized DNA repair activities in healthy aged individuals. Methods Amalaki rasayana was prepared fresh and healthy aged randomized human volunteers were administrated with either rasayana or placebo for 45 days strictly as per the traditional text. The DNA repair was analyzed in peripheral blood mononuclear cells before and after rasayana administration and after 45 days post-rasayana treatment regimen. UVC-induced DNA strand break repair (DSBR) based on extent of DNA unwinding by fluorometric analysis, nucleotide excision repair (NER) by flow cytometry and constitutive base excision repair (BER) by gap filling method were analyzed. Results Amalaki rasayana administration stably maintained/enhanced the DSBR in aged individuals. There were no adverse side effects. Further, subjects with different body mass index showed differential DNA strand break repair capacity. No change in unscheduled DNA synthesis during NER and BER was observed between the groups. Conclusion Intake of Amalaki rasayana by aged individuals showed stable maintenance of DNA strand break repair without toxic effects. However, there was no change in nucleotide and base excision repair activities. Results warrant further studies on the effects of Amalaki rasayana on DSBR activities.",
author = "Udupi Vishwanatha and Guruprasad, {Kanive P.} and Gopinath, {Puthiya M.} and Acharya, {Raviraj V.} and Prasanna, {Bokkasa V.} and Jayakrishna Nayak and Rajeshwari Ganesh and Jayalaxmi Rao and Rashmi Shree and Suchitra Anchan and Raghu, {Kothanahalli S.} and Joshi, {Manjunath B.} and Puspendu Paladhi and Varier, {Panniampilly M.} and Kollath Muraleedharan and Muraleedharan, {Thrikovil S.} and Kapaettu Satyamoorthy",
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Vishwanatha, U, Guruprasad, KP, Gopinath, PM, Acharya, RV, Prasanna, BV, Nayak, J, Ganesh, R, Rao, J, Shree, R, Anchan, S, Raghu, KS, Joshi, MB, Paladhi, P, Varier, PM, Muraleedharan, K, Muraleedharan, TS & Satyamoorthy, K 2016, 'Effect of Amalaki rasayana on DNA damage and repair in randomized aged human individuals', Journal of Ethnopharmacology, vol. 191, pp. 387-397. https://doi.org/10.1016/j.jep.2016.06.062

Effect of Amalaki rasayana on DNA damage and repair in randomized aged human individuals. / Vishwanatha, Udupi; Guruprasad, Kanive P.; Gopinath, Puthiya M.; Acharya, Raviraj V.; Prasanna, Bokkasa V.; Nayak, Jayakrishna; Ganesh, Rajeshwari; Rao, Jayalaxmi; Shree, Rashmi; Anchan, Suchitra; Raghu, Kothanahalli S.; Joshi, Manjunath B.; Paladhi, Puspendu; Varier, Panniampilly M.; Muraleedharan, Kollath; Muraleedharan, Thrikovil S.; Satyamoorthy, Kapaettu.

In: Journal of Ethnopharmacology, Vol. 191, 15.09.2016, p. 387-397.

Research output: Contribution to journalArticle

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T1 - Effect of Amalaki rasayana on DNA damage and repair in randomized aged human individuals

AU - Vishwanatha, Udupi

AU - Guruprasad, Kanive P.

AU - Gopinath, Puthiya M.

AU - Acharya, Raviraj V.

AU - Prasanna, Bokkasa V.

AU - Nayak, Jayakrishna

AU - Ganesh, Rajeshwari

AU - Rao, Jayalaxmi

AU - Shree, Rashmi

AU - Anchan, Suchitra

AU - Raghu, Kothanahalli S.

AU - Joshi, Manjunath B.

AU - Paladhi, Puspendu

AU - Varier, Panniampilly M.

AU - Muraleedharan, Kollath

AU - Muraleedharan, Thrikovil S.

AU - Satyamoorthy, Kapaettu

PY - 2016/9/15

Y1 - 2016/9/15

N2 - Ethnopharmacological relevance Preparations from Phyllanthus emblica called Amalaki rasayana is used in the Indian traditional medicinal system of Ayurveda for healthy living in elderly. The biological effects and its mechanisms are not fully understood. Since the diminishing DNA repair is the hallmark of ageing, we tested the influence of Amalaki rasayana on recognized DNA repair activities in healthy aged individuals. Methods Amalaki rasayana was prepared fresh and healthy aged randomized human volunteers were administrated with either rasayana or placebo for 45 days strictly as per the traditional text. The DNA repair was analyzed in peripheral blood mononuclear cells before and after rasayana administration and after 45 days post-rasayana treatment regimen. UVC-induced DNA strand break repair (DSBR) based on extent of DNA unwinding by fluorometric analysis, nucleotide excision repair (NER) by flow cytometry and constitutive base excision repair (BER) by gap filling method were analyzed. Results Amalaki rasayana administration stably maintained/enhanced the DSBR in aged individuals. There were no adverse side effects. Further, subjects with different body mass index showed differential DNA strand break repair capacity. No change in unscheduled DNA synthesis during NER and BER was observed between the groups. Conclusion Intake of Amalaki rasayana by aged individuals showed stable maintenance of DNA strand break repair without toxic effects. However, there was no change in nucleotide and base excision repair activities. Results warrant further studies on the effects of Amalaki rasayana on DSBR activities.

AB - Ethnopharmacological relevance Preparations from Phyllanthus emblica called Amalaki rasayana is used in the Indian traditional medicinal system of Ayurveda for healthy living in elderly. The biological effects and its mechanisms are not fully understood. Since the diminishing DNA repair is the hallmark of ageing, we tested the influence of Amalaki rasayana on recognized DNA repair activities in healthy aged individuals. Methods Amalaki rasayana was prepared fresh and healthy aged randomized human volunteers were administrated with either rasayana or placebo for 45 days strictly as per the traditional text. The DNA repair was analyzed in peripheral blood mononuclear cells before and after rasayana administration and after 45 days post-rasayana treatment regimen. UVC-induced DNA strand break repair (DSBR) based on extent of DNA unwinding by fluorometric analysis, nucleotide excision repair (NER) by flow cytometry and constitutive base excision repair (BER) by gap filling method were analyzed. Results Amalaki rasayana administration stably maintained/enhanced the DSBR in aged individuals. There were no adverse side effects. Further, subjects with different body mass index showed differential DNA strand break repair capacity. No change in unscheduled DNA synthesis during NER and BER was observed between the groups. Conclusion Intake of Amalaki rasayana by aged individuals showed stable maintenance of DNA strand break repair without toxic effects. However, there was no change in nucleotide and base excision repair activities. Results warrant further studies on the effects of Amalaki rasayana on DSBR activities.

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