Effect of azidothymidine on the radiation-induced LDH release in HeLa cells

G. C. Jagetia, R. Aruna, B. S. Nayak

Research output: Contribution to journalArticle

Abstract

Treatment of HeLa cells with various concentrations of 0, 0.01, 0.1, 1, 10, and 100 μM AZT resulted in a concentration dependent elevation in the LDH release at 0, 0.5, 1, 2 and 4 h post-treatment. An elevation of 1.7 to 9.2 fold in LDH content was observed at 1 h post-treatment depending on the drug concentration. Similarly, treatment of HeLa cells with 0.1 μM AZT before irradiation caused an irradiation dose dependent increase in the LDH release in AZT + irradiation groups. This increase in LDH release was approximately two fold greater at 0 h post-irradiation in AZT + irradiation group, when compared with the PBS + irradiation group. This trend of elevation in LDH release continued up to 2 h, except 2 and 3 Gy, where it was 1.7 fold in the former group when compared with the latter. However, a peak level of LDH release was observed at 0 h post-irradiation.

Original languageEnglish
Pages (from-to)837-840
Number of pages4
JournalPharmazie
Volume55
Issue number11
Publication statusPublished - 05-12-2000
Externally publishedYes

Fingerprint

Zidovudine
HeLa Cells
Radiation
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Jagetia, G. C., Aruna, R., & Nayak, B. S. (2000). Effect of azidothymidine on the radiation-induced LDH release in HeLa cells. Pharmazie, 55(11), 837-840.
Jagetia, G. C. ; Aruna, R. ; Nayak, B. S. / Effect of azidothymidine on the radiation-induced LDH release in HeLa cells. In: Pharmazie. 2000 ; Vol. 55, No. 11. pp. 837-840.
@article{09996a46279c4f19bda76a964d8dd858,
title = "Effect of azidothymidine on the radiation-induced LDH release in HeLa cells",
abstract = "Treatment of HeLa cells with various concentrations of 0, 0.01, 0.1, 1, 10, and 100 μM AZT resulted in a concentration dependent elevation in the LDH release at 0, 0.5, 1, 2 and 4 h post-treatment. An elevation of 1.7 to 9.2 fold in LDH content was observed at 1 h post-treatment depending on the drug concentration. Similarly, treatment of HeLa cells with 0.1 μM AZT before irradiation caused an irradiation dose dependent increase in the LDH release in AZT + irradiation groups. This increase in LDH release was approximately two fold greater at 0 h post-irradiation in AZT + irradiation group, when compared with the PBS + irradiation group. This trend of elevation in LDH release continued up to 2 h, except 2 and 3 Gy, where it was 1.7 fold in the former group when compared with the latter. However, a peak level of LDH release was observed at 0 h post-irradiation.",
author = "Jagetia, {G. C.} and R. Aruna and Nayak, {B. S.}",
year = "2000",
month = "12",
day = "5",
language = "English",
volume = "55",
pages = "837--840",
journal = "Die Pharmazie",
issn = "0031-7144",
publisher = "Govi-Verlag Pharmazeutischer Verlag GmbH",
number = "11",

}

Jagetia, GC, Aruna, R & Nayak, BS 2000, 'Effect of azidothymidine on the radiation-induced LDH release in HeLa cells', Pharmazie, vol. 55, no. 11, pp. 837-840.

Effect of azidothymidine on the radiation-induced LDH release in HeLa cells. / Jagetia, G. C.; Aruna, R.; Nayak, B. S.

In: Pharmazie, Vol. 55, No. 11, 05.12.2000, p. 837-840.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of azidothymidine on the radiation-induced LDH release in HeLa cells

AU - Jagetia, G. C.

AU - Aruna, R.

AU - Nayak, B. S.

PY - 2000/12/5

Y1 - 2000/12/5

N2 - Treatment of HeLa cells with various concentrations of 0, 0.01, 0.1, 1, 10, and 100 μM AZT resulted in a concentration dependent elevation in the LDH release at 0, 0.5, 1, 2 and 4 h post-treatment. An elevation of 1.7 to 9.2 fold in LDH content was observed at 1 h post-treatment depending on the drug concentration. Similarly, treatment of HeLa cells with 0.1 μM AZT before irradiation caused an irradiation dose dependent increase in the LDH release in AZT + irradiation groups. This increase in LDH release was approximately two fold greater at 0 h post-irradiation in AZT + irradiation group, when compared with the PBS + irradiation group. This trend of elevation in LDH release continued up to 2 h, except 2 and 3 Gy, where it was 1.7 fold in the former group when compared with the latter. However, a peak level of LDH release was observed at 0 h post-irradiation.

AB - Treatment of HeLa cells with various concentrations of 0, 0.01, 0.1, 1, 10, and 100 μM AZT resulted in a concentration dependent elevation in the LDH release at 0, 0.5, 1, 2 and 4 h post-treatment. An elevation of 1.7 to 9.2 fold in LDH content was observed at 1 h post-treatment depending on the drug concentration. Similarly, treatment of HeLa cells with 0.1 μM AZT before irradiation caused an irradiation dose dependent increase in the LDH release in AZT + irradiation groups. This increase in LDH release was approximately two fold greater at 0 h post-irradiation in AZT + irradiation group, when compared with the PBS + irradiation group. This trend of elevation in LDH release continued up to 2 h, except 2 and 3 Gy, where it was 1.7 fold in the former group when compared with the latter. However, a peak level of LDH release was observed at 0 h post-irradiation.

UR - http://www.scopus.com/inward/record.url?scp=0033694545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033694545&partnerID=8YFLogxK

M3 - Article

VL - 55

SP - 837

EP - 840

JO - Die Pharmazie

JF - Die Pharmazie

SN - 0031-7144

IS - 11

ER -

Jagetia GC, Aruna R, Nayak BS. Effect of azidothymidine on the radiation-induced LDH release in HeLa cells. Pharmazie. 2000 Dec 5;55(11):837-840.