Effect of cholesterol on size distribution of freeze-thaw extruded liposomes

S. Agarwal, G. Subramanian, P. Shetiya, N. Udupa

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freeze-thawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196° followed by 3 min thawing at 50° resulted in an anomalously large particle size for egg phosphatidylcholine/cholesterol formulations. When egg phosphatidylcholine/cholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.

Original languageEnglish
Pages (from-to)450-452
Number of pages3
JournalIndian Journal of Pharmaceutical Sciences
Volume66
Issue number4
Publication statusPublished - 2004

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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