@article{d0cfe43f1f8b49e8bd1e64db32e58f10,
title = "Effect of cholesterol on size distribution of freeze-thaw extruded liposomes",
abstract = "The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freeze-thawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196° followed by 3 min thawing at 50° resulted in an anomalously large particle size for egg phosphatidylcholine/cholesterol formulations. When egg phosphatidylcholine/cholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.",
author = "S. Agarwal and G. Subramanian and P. Shetiya and N. Udupa",
note = "Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Udupa, N.; College of Pharmaceutical Sciences, MAHE, Manipal-576119, India; email: n.udupa@cops.manipal.edu References: Bangham, A.D., Hill, M.W., Miller, A.G., (1974) Methods in Membrane Biology, 1, p. 1. , Korn, E.D., Eds; Plenum Press, New York; Hope, M.J., Bally, M.B., Webb, G., Cullis, P.R., (1985) Blochim. Biophys. Acta, 812, p. 55; Mayer, L.D., Hope, M.J., Cullis, P.R., (1986) Biochim. Biophys. Acta, 858, p. 161; Elorza, B., Elorza, M.A., Sainz, M.C., Chantrez, J.R., (1993) J. Microencap., 10, p. 237; Talsma, H., Van Steenbergen, M.J., Salemink, P.J.M., Crommelin, D.J.A., (1991) Pharm. Res., 8, p. 1021; Ozer, Y., Talsma, H., Crommelin, D.J.A., Hincal, A.A., (1988) Acta Pharm. Technol., 34, p. 129; Nagata, M., Yotsuyanagi, T., Ikeda, K., (1986) Chem. Pharm. Bull., 34, p. 1391; Crowe, J.H., Crowe, L.M., Carpenter, J.F., Wistrom, C.A., (1987) Biochem. J., 242, p. 1; Oldfield, E., Chapman, D., (1972) FEBS Lett, 23, p. 285; Finklestein, A., Cass, A., (1967) Nature, 216, p. 717; Henry-Michellard, S., Ter-Minassian-Saraga, L., Poly, P.A., Delattre, J., Pusieux, F., (1985) Colloids Sufaces, 14, p. 269",
year = "2004",
language = "English",
volume = "66",
pages = "450--452",
journal = "Indian Journal of Pharmaceutical Sciences",
issn = "0250-474X",
publisher = "Medknow Publications and Media Pvt. Ltd",
number = "4",
}