Effect of cholesterol on size distribution of freeze-thaw extruded liposomes

S. Agarwal, G. Subramanian, P. Shetiya, N. Udupa

Research output: Contribution to journalArticle

Abstract

The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freeze-thawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196° followed by 3 min thawing at 50° resulted in an anomalously large particle size for egg phosphatidylcholine/cholesterol formulations. When egg phosphatidylcholine/cholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.

Original languageEnglish
Pages (from-to)450-452
Number of pages3
JournalIndian Journal of Pharmaceutical Sciences
Volume66
Issue number4
Publication statusPublished - 2004

Fingerprint

Phosphatidylcholines
Liposomes
Ovum
Cholesterol
Particle Size
Freezing
Lasers
Nitrogen

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Agarwal, S. ; Subramanian, G. ; Shetiya, P. ; Udupa, N. / Effect of cholesterol on size distribution of freeze-thaw extruded liposomes. In: Indian Journal of Pharmaceutical Sciences. 2004 ; Vol. 66, No. 4. pp. 450-452.
@article{d0cfe43f1f8b49e8bd1e64db32e58f10,
title = "Effect of cholesterol on size distribution of freeze-thaw extruded liposomes",
abstract = "The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freeze-thawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196° followed by 3 min thawing at 50° resulted in an anomalously large particle size for egg phosphatidylcholine/cholesterol formulations. When egg phosphatidylcholine/cholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.",
author = "S. Agarwal and G. Subramanian and P. Shetiya and N. Udupa",
note = "Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Udupa, N.; College of Pharmaceutical Sciences, MAHE, Manipal-576119, India; email: n.udupa@cops.manipal.edu References: Bangham, A.D., Hill, M.W., Miller, A.G., (1974) Methods in Membrane Biology, 1, p. 1. , Korn, E.D., Eds; Plenum Press, New York; Hope, M.J., Bally, M.B., Webb, G., Cullis, P.R., (1985) Blochim. Biophys. Acta, 812, p. 55; Mayer, L.D., Hope, M.J., Cullis, P.R., (1986) Biochim. Biophys. Acta, 858, p. 161; Elorza, B., Elorza, M.A., Sainz, M.C., Chantrez, J.R., (1993) J. Microencap., 10, p. 237; Talsma, H., Van Steenbergen, M.J., Salemink, P.J.M., Crommelin, D.J.A., (1991) Pharm. Res., 8, p. 1021; Ozer, Y., Talsma, H., Crommelin, D.J.A., Hincal, A.A., (1988) Acta Pharm. Technol., 34, p. 129; Nagata, M., Yotsuyanagi, T., Ikeda, K., (1986) Chem. Pharm. Bull., 34, p. 1391; Crowe, J.H., Crowe, L.M., Carpenter, J.F., Wistrom, C.A., (1987) Biochem. J., 242, p. 1; Oldfield, E., Chapman, D., (1972) FEBS Lett, 23, p. 285; Finklestein, A., Cass, A., (1967) Nature, 216, p. 717; Henry-Michellard, S., Ter-Minassian-Saraga, L., Poly, P.A., Delattre, J., Pusieux, F., (1985) Colloids Sufaces, 14, p. 269",
year = "2004",
language = "English",
volume = "66",
pages = "450--452",
journal = "Indian Journal of Pharmaceutical Sciences",
issn = "0250-474X",
publisher = "Medknow Publications and Media Pvt. Ltd",
number = "4",

}

Effect of cholesterol on size distribution of freeze-thaw extruded liposomes. / Agarwal, S.; Subramanian, G.; Shetiya, P.; Udupa, N.

In: Indian Journal of Pharmaceutical Sciences, Vol. 66, No. 4, 2004, p. 450-452.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of cholesterol on size distribution of freeze-thaw extruded liposomes

AU - Agarwal, S.

AU - Subramanian, G.

AU - Shetiya, P.

AU - Udupa, N.

N1 - Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Udupa, N.; College of Pharmaceutical Sciences, MAHE, Manipal-576119, India; email: n.udupa@cops.manipal.edu References: Bangham, A.D., Hill, M.W., Miller, A.G., (1974) Methods in Membrane Biology, 1, p. 1. , Korn, E.D., Eds; Plenum Press, New York; Hope, M.J., Bally, M.B., Webb, G., Cullis, P.R., (1985) Blochim. Biophys. Acta, 812, p. 55; Mayer, L.D., Hope, M.J., Cullis, P.R., (1986) Biochim. Biophys. Acta, 858, p. 161; Elorza, B., Elorza, M.A., Sainz, M.C., Chantrez, J.R., (1993) J. Microencap., 10, p. 237; Talsma, H., Van Steenbergen, M.J., Salemink, P.J.M., Crommelin, D.J.A., (1991) Pharm. Res., 8, p. 1021; Ozer, Y., Talsma, H., Crommelin, D.J.A., Hincal, A.A., (1988) Acta Pharm. Technol., 34, p. 129; Nagata, M., Yotsuyanagi, T., Ikeda, K., (1986) Chem. Pharm. Bull., 34, p. 1391; Crowe, J.H., Crowe, L.M., Carpenter, J.F., Wistrom, C.A., (1987) Biochem. J., 242, p. 1; Oldfield, E., Chapman, D., (1972) FEBS Lett, 23, p. 285; Finklestein, A., Cass, A., (1967) Nature, 216, p. 717; Henry-Michellard, S., Ter-Minassian-Saraga, L., Poly, P.A., Delattre, J., Pusieux, F., (1985) Colloids Sufaces, 14, p. 269

PY - 2004

Y1 - 2004

N2 - The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freeze-thawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196° followed by 3 min thawing at 50° resulted in an anomalously large particle size for egg phosphatidylcholine/cholesterol formulations. When egg phosphatidylcholine/cholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.

AB - The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freeze-thawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196° followed by 3 min thawing at 50° resulted in an anomalously large particle size for egg phosphatidylcholine/cholesterol formulations. When egg phosphatidylcholine/cholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.

UR - http://www.scopus.com/inward/record.url?scp=77952834790&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952834790&partnerID=8YFLogxK

M3 - Article

VL - 66

SP - 450

EP - 452

JO - Indian Journal of Pharmaceutical Sciences

JF - Indian Journal of Pharmaceutical Sciences

SN - 0250-474X

IS - 4

ER -