Effect of cholesterol on size distribution of freeze-thaw extruded liposomes

S. Agarwal, G. Subramanian, P. Shetiya, N. Udupa

Research output: Contribution to journalArticle

Abstract

The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freeze-thawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196° followed by 3 min thawing at 50° resulted in an anomalously large particle size for egg phosphatidylcholine/cholesterol formulations. When egg phosphatidylcholine/cholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.

Original languageEnglish
Pages (from-to)450-452
Number of pages3
JournalIndian Journal of Pharmaceutical Sciences
Volume66
Issue number4
Publication statusPublished - 2004

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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