Incubation of rat renal cortical slices with 3.3 mM cisplatin produced concentration - dependent decrease in total protein. The reduction in protein content was significant only after 30 min. To find out the role of reactive oxygen species (ROS), incubation was carried out in the presence of antioxidants.The water soluble analog of Vitamin E, trolox, protected the cortical slices against the cisplatin effect but ascorbic acid failed to produce any effect. Similarly, catalase and hydroxyl radical scavengers like dimethyl sulphoxide (DMSO), mannitol produced protection but superoxide dismutase (SOD) did not show protection. In conclusion, this study indicates that cisplatin causes decrease in the protein content in renal cortical slices and this decrease may be mediated through reactive oxygen species.
|Number of pages||4|
|Journal||Indian Journal of Pharmaceutical Sciences|
|Publication status||Published - 1998|