Effect of Cisplatin on Total Protein in Rat Kidney Cortical Slices

M Rao, M.N.A. Rao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Incubation of rat renal cortical slices with 3.3 mM cisplatin produced concentration - dependent decrease in total protein. The reduction in protein content was significant only after 30 min. To find out the role of reactive oxygen species (ROS), incubation was carried out in the presence of antioxidants.The water soluble analog of Vitamin E, trolox, protected the cortical slices against the cisplatin effect but ascorbic acid failed to produce any effect. Similarly, catalase and hydroxyl radical scavengers like dimethyl sulphoxide (DMSO), mannitol produced protection but superoxide dismutase (SOD) did not show protection. In conclusion, this study indicates that cisplatin causes decrease in the protein content in renal cortical slices and this decrease may be mediated through reactive oxygen species.
Original languageUndefined/Unknown
Pages (from-to)132-135
Number of pages4
JournalIndian Journal of Pharmaceutical Sciences
Volume60
Issue number3
Publication statusPublished - 1998

Cite this

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title = "Effect of Cisplatin on Total Protein in Rat Kidney Cortical Slices",
abstract = "Incubation of rat renal cortical slices with 3.3 mM cisplatin produced concentration - dependent decrease in total protein. The reduction in protein content was significant only after 30 min. To find out the role of reactive oxygen species (ROS), incubation was carried out in the presence of antioxidants.The water soluble analog of Vitamin E, trolox, protected the cortical slices against the cisplatin effect but ascorbic acid failed to produce any effect. Similarly, catalase and hydroxyl radical scavengers like dimethyl sulphoxide (DMSO), mannitol produced protection but superoxide dismutase (SOD) did not show protection. In conclusion, this study indicates that cisplatin causes decrease in the protein content in renal cortical slices and this decrease may be mediated through reactive oxygen species.",
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Effect of Cisplatin on Total Protein in Rat Kidney Cortical Slices. / Rao, M; Rao, M.N.A.

In: Indian Journal of Pharmaceutical Sciences, Vol. 60, No. 3, 1998, p. 132-135.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of Cisplatin on Total Protein in Rat Kidney Cortical Slices

AU - Rao, M

AU - Rao, M.N.A.

N1 - cited By 1

PY - 1998

Y1 - 1998

N2 - Incubation of rat renal cortical slices with 3.3 mM cisplatin produced concentration - dependent decrease in total protein. The reduction in protein content was significant only after 30 min. To find out the role of reactive oxygen species (ROS), incubation was carried out in the presence of antioxidants.The water soluble analog of Vitamin E, trolox, protected the cortical slices against the cisplatin effect but ascorbic acid failed to produce any effect. Similarly, catalase and hydroxyl radical scavengers like dimethyl sulphoxide (DMSO), mannitol produced protection but superoxide dismutase (SOD) did not show protection. In conclusion, this study indicates that cisplatin causes decrease in the protein content in renal cortical slices and this decrease may be mediated through reactive oxygen species.

AB - Incubation of rat renal cortical slices with 3.3 mM cisplatin produced concentration - dependent decrease in total protein. The reduction in protein content was significant only after 30 min. To find out the role of reactive oxygen species (ROS), incubation was carried out in the presence of antioxidants.The water soluble analog of Vitamin E, trolox, protected the cortical slices against the cisplatin effect but ascorbic acid failed to produce any effect. Similarly, catalase and hydroxyl radical scavengers like dimethyl sulphoxide (DMSO), mannitol produced protection but superoxide dismutase (SOD) did not show protection. In conclusion, this study indicates that cisplatin causes decrease in the protein content in renal cortical slices and this decrease may be mediated through reactive oxygen species.

M3 - Article

VL - 60

SP - 132

EP - 135

JO - Indian Journal of Pharmaceutical Sciences

JF - Indian Journal of Pharmaceutical Sciences

SN - 0250-474X

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ER -