Abstract

Objective: To test whether sperm with abnormal head morphology are more likely to undergo DNA damage and/or chromatin modification during the process of freeze-thawing. Design: In this prospective study, the semen samples from forty-four men attending the infertility clinic were included. Samples were divided into aliquots to allow direct comparison of fresh and frozen spermatozoa from the same ejaculate. The sperm morphology and the sperm DNA damage were evaluated before and after cryopreservation. The relationship between sperm head abnormalities and freeze-thaw-induced DNA modification was assessed. Setting(s): University hospital fertility center. Patient(s): Men attending infertility clinic for semen analysis. Intervention(s): The normospermic and teratospermic semen samples were evaluated for DNA damage before and after cryopreservation by comet assay and acridine orange bindability test. Main Outcome Measure(s): Elucidation of association between sperm morphologic defect and cryodamage. Result(s): A threefold increase in the amount of DNA damage was observed in teratospermic samples compared with their normospermic counterparts, indicating a higher susceptibility of morphologically abnormal sperm to cryodamage. Conclusion(s): The susceptibility of morphologically abnormal sperm to DNA damage/chromatin modification during the freeze-thaw process is significantly higher than that of sperm with normal morphology.

Original languageEnglish
Pages (from-to)1723-1727
Number of pages5
JournalFertility and Sterility
Volume89
Issue number6
DOIs
Publication statusPublished - 06-2008

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Cryopreservation
Spermatozoa
DNA Damage
DNA
Semen
Infertility
Chromatin
Sperm Head
Acridine Orange
Comet Assay
Semen Analysis
Teratozoospermia
Fertility
Head
Outcome Assessment (Health Care)
Prospective Studies

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

@article{8775aaab807040319d75bc0ee40bdd75,
title = "Effect of cryopreservation on sperm DNA integrity in patients with teratospermia",
abstract = "Objective: To test whether sperm with abnormal head morphology are more likely to undergo DNA damage and/or chromatin modification during the process of freeze-thawing. Design: In this prospective study, the semen samples from forty-four men attending the infertility clinic were included. Samples were divided into aliquots to allow direct comparison of fresh and frozen spermatozoa from the same ejaculate. The sperm morphology and the sperm DNA damage were evaluated before and after cryopreservation. The relationship between sperm head abnormalities and freeze-thaw-induced DNA modification was assessed. Setting(s): University hospital fertility center. Patient(s): Men attending infertility clinic for semen analysis. Intervention(s): The normospermic and teratospermic semen samples were evaluated for DNA damage before and after cryopreservation by comet assay and acridine orange bindability test. Main Outcome Measure(s): Elucidation of association between sperm morphologic defect and cryodamage. Result(s): A threefold increase in the amount of DNA damage was observed in teratospermic samples compared with their normospermic counterparts, indicating a higher susceptibility of morphologically abnormal sperm to cryodamage. Conclusion(s): The susceptibility of morphologically abnormal sperm to DNA damage/chromatin modification during the freeze-thaw process is significantly higher than that of sperm with normal morphology.",
author = "Guruprasad Kalthur and Adiga, {Satish Kumar} and Dinesh Upadhya and Satish Rao and Pratap Kumar",
year = "2008",
month = "6",
doi = "10.1016/j.fertnstert.2007.06.087",
language = "English",
volume = "89",
pages = "1723--1727",
journal = "Fertility and Sterility",
issn = "0015-0282",
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TY - JOUR

T1 - Effect of cryopreservation on sperm DNA integrity in patients with teratospermia

AU - Kalthur, Guruprasad

AU - Adiga, Satish Kumar

AU - Upadhya, Dinesh

AU - Rao, Satish

AU - Kumar, Pratap

PY - 2008/6

Y1 - 2008/6

N2 - Objective: To test whether sperm with abnormal head morphology are more likely to undergo DNA damage and/or chromatin modification during the process of freeze-thawing. Design: In this prospective study, the semen samples from forty-four men attending the infertility clinic were included. Samples were divided into aliquots to allow direct comparison of fresh and frozen spermatozoa from the same ejaculate. The sperm morphology and the sperm DNA damage were evaluated before and after cryopreservation. The relationship between sperm head abnormalities and freeze-thaw-induced DNA modification was assessed. Setting(s): University hospital fertility center. Patient(s): Men attending infertility clinic for semen analysis. Intervention(s): The normospermic and teratospermic semen samples were evaluated for DNA damage before and after cryopreservation by comet assay and acridine orange bindability test. Main Outcome Measure(s): Elucidation of association between sperm morphologic defect and cryodamage. Result(s): A threefold increase in the amount of DNA damage was observed in teratospermic samples compared with their normospermic counterparts, indicating a higher susceptibility of morphologically abnormal sperm to cryodamage. Conclusion(s): The susceptibility of morphologically abnormal sperm to DNA damage/chromatin modification during the freeze-thaw process is significantly higher than that of sperm with normal morphology.

AB - Objective: To test whether sperm with abnormal head morphology are more likely to undergo DNA damage and/or chromatin modification during the process of freeze-thawing. Design: In this prospective study, the semen samples from forty-four men attending the infertility clinic were included. Samples were divided into aliquots to allow direct comparison of fresh and frozen spermatozoa from the same ejaculate. The sperm morphology and the sperm DNA damage were evaluated before and after cryopreservation. The relationship between sperm head abnormalities and freeze-thaw-induced DNA modification was assessed. Setting(s): University hospital fertility center. Patient(s): Men attending infertility clinic for semen analysis. Intervention(s): The normospermic and teratospermic semen samples were evaluated for DNA damage before and after cryopreservation by comet assay and acridine orange bindability test. Main Outcome Measure(s): Elucidation of association between sperm morphologic defect and cryodamage. Result(s): A threefold increase in the amount of DNA damage was observed in teratospermic samples compared with their normospermic counterparts, indicating a higher susceptibility of morphologically abnormal sperm to cryodamage. Conclusion(s): The susceptibility of morphologically abnormal sperm to DNA damage/chromatin modification during the freeze-thaw process is significantly higher than that of sperm with normal morphology.

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