Effect of culture conditions and kinetic studies on extracellular tannase production by Lactobacillus plantarum MTCC 1407

Natarajan Kannan, Rajendran Aravindan, Thangavelu Viruthagiri

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Production of tannase and microbial biomass by Lactobacillus plantarum MTCC 1407 was studied in submerged batch fermentation. The tannase production was performed in different media compositions maintaining the fermentation conditions constant at 30°C, initial pH 5.5 and agitation speed at 120 rpm. Maximum tannase activity of 5.22 U mL-l was obtained at 24 h in M3 medium containing the following composition (g L-l): tannic acid, 10; glucose, 1; NH 4Cl, 3; MgSO 4.7H 2O, 2; KH 2PO 4, 0.5; K 2HPO 4, 0.5; and CaCl 2, l. The medium optimization studies show that the tannic acid (1% w/v) as inducer along with glucose (0.1% w/v) as carbon source gave maximum tannase activity. Unstructured kinetic models, namely, logistic model for cell growth and Luedeking-Piret model for tannase production, were used to predict the fermentation kinetics. The estimated values of the kinetic model parameters, α and β for tannase production indicated that the tannase production by L. plantarum was growth associated.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalIndian Journal of Biotechnology
Volume10
Issue number3
Publication statusPublished - 01-07-2011

Fingerprint

tannase
Lactobacillus plantarum
kinetics
Kinetics
Fermentation
Enzyme kinetics
Glucose
Tannins
tannins
Acids
Cell growth
fermentation
Chemical analysis
submerged fermentation
batch fermentation
glucose
Logistics
Biomass
agitation
logit analysis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Plant Science

Cite this

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Effect of culture conditions and kinetic studies on extracellular tannase production by Lactobacillus plantarum MTCC 1407. / Kannan, Natarajan; Aravindan, Rajendran; Viruthagiri, Thangavelu.

In: Indian Journal of Biotechnology, Vol. 10, No. 3, 01.07.2011, p. 321-328.

Research output: Contribution to journalArticle

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