Effect of curcumin and curcumin copper complex (1:1) on radiation-induced changes of anti-oxidant enzymes levels in the livers of swiss albino mice

P.R. Koiram, V.P. Veerapur, A. Kunwar, B. Mishra, A. Barik, I.K. Priyadarsini, U.K. Mazhuvancherry

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Radiation Antioxidant enzymes Curcumin Curcumin copper complex GSH. The effect of mononuclear copper (II) complex of curcumin in 1:1 stoichiometry (hereafter referred to as complex) administered 30 min before γ-irradiation (4.5 Gy) on alterations in antioxidant and Thiobarbituric acid reactive substances (TBARS) levels in livers was studied in comparison to curcumin at a dose of 50 mg/kg. The different antioxidants like GSH, GST, catalase, SOD, TBARS and total thiols were estimated in the liver homogenates excised at different time intervals (1, 2 and 4 h) post irradiation using colorimetric methods. There was a radiation-induced decrease in the levels of all the studied enzymes at 1 h post irradiation, while an increase was observed at later time points. Both curcumin and complex treatment in sham-irradiated mice decreased the levels of GSH and total thiols, whereas there was an increase in the levels of catalase, GST and SOD compared to normal control. Under the influence of irradiation, both curcumin and complex treatment protected the decline in the levels of GSH, GST, SOD, catalase and total thiols, and inhibited radiation-induced lipid peroxidation. Further, the complex was found to be more effective in protecting the enzymes at 1 h post irradiation compared to curcumin treated group. This may be due to the higher rate constants of the complex compared to curcumin for their reactions with various free radicals.
Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalJournal of Radiation Research
Volume48
Issue number3
DOIs
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Curcumin
liver
Oxidants
mice
enzymes
Copper
catalase
Radiation
antioxidants
copper
irradiation
thiols
Liver
Enzymes
radiation
Sulfhydryl Compounds
Catalase
Thiobarbituric Acid Reactive Substances
Antioxidants
acids

Cite this

Koiram, P. R., Veerapur, V. P., Kunwar, A., Mishra, B., Barik, A., Priyadarsini, I. K., & Mazhuvancherry, U. K. (2007). Effect of curcumin and curcumin copper complex (1:1) on radiation-induced changes of anti-oxidant enzymes levels in the livers of swiss albino mice. Journal of Radiation Research, 48(3), 241-245. https://doi.org/10.1269/jrr.06103
Koiram, P.R. ; Veerapur, V.P. ; Kunwar, A. ; Mishra, B. ; Barik, A. ; Priyadarsini, I.K. ; Mazhuvancherry, U.K. / Effect of curcumin and curcumin copper complex (1:1) on radiation-induced changes of anti-oxidant enzymes levels in the livers of swiss albino mice. In: Journal of Radiation Research. 2007 ; Vol. 48, No. 3. pp. 241-245.
@article{74716c466d0f4bb7b4a2dbf51988d9a9,
title = "Effect of curcumin and curcumin copper complex (1:1) on radiation-induced changes of anti-oxidant enzymes levels in the livers of swiss albino mice",
abstract = "Radiation Antioxidant enzymes Curcumin Curcumin copper complex GSH. The effect of mononuclear copper (II) complex of curcumin in 1:1 stoichiometry (hereafter referred to as complex) administered 30 min before γ-irradiation (4.5 Gy) on alterations in antioxidant and Thiobarbituric acid reactive substances (TBARS) levels in livers was studied in comparison to curcumin at a dose of 50 mg/kg. The different antioxidants like GSH, GST, catalase, SOD, TBARS and total thiols were estimated in the liver homogenates excised at different time intervals (1, 2 and 4 h) post irradiation using colorimetric methods. There was a radiation-induced decrease in the levels of all the studied enzymes at 1 h post irradiation, while an increase was observed at later time points. Both curcumin and complex treatment in sham-irradiated mice decreased the levels of GSH and total thiols, whereas there was an increase in the levels of catalase, GST and SOD compared to normal control. Under the influence of irradiation, both curcumin and complex treatment protected the decline in the levels of GSH, GST, SOD, catalase and total thiols, and inhibited radiation-induced lipid peroxidation. Further, the complex was found to be more effective in protecting the enzymes at 1 h post irradiation compared to curcumin treated group. This may be due to the higher rate constants of the complex compared to curcumin for their reactions with various free radicals.",
author = "P.R. Koiram and V.P. Veerapur and A. Kunwar and B. Mishra and A. Barik and I.K. Priyadarsini and U.K. Mazhuvancherry",
note = "Cited By :13 Export Date: 10 November 2017 Correspondence Address: Mazhuvancherry, U.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal - 576 104, India; email: mkunnikrishnan@gmail.com Chemicals/CAS: catalase, 9001-05-2; copper, 15158-11-9, 7440-50-8; curcumin, 458-37-7; glutathione transferase, 50812-37-8; Antioxidants; Catalase, 1.11.1.6; Copper, 7440-50-8; Curcumin, 458-37-7; Glutathione Transferase, 2.5.1.18; Radiation-Protective Agents References: Abrescia, P., Golino, P., Free radicals and antioxidants in cardiovascular diseases (2005) Expert. Rev. Cardiovasc. Ther, 3, pp. 159-171; Aggarwal, B.B., Kumar, A., Bharti, A.C., Anticancer potential of curcumin: Preclinical and clinical studies (2003) Anticancer Res, 23, pp. 363-398; Sharma, R.A., Gescher, A.J., Steward, W.P., Curcumin: The story so far (2005) Eur. J. Cancer, 41, pp. 1955-1968; Shishodia, S., Sethi, G., Aggarwal, B.B., Curcumin: Getting back to roots (2005) Ann. N.Y. Acad. Sci, 1056, pp. 206-217; Sato, Y., Hotta, N., Sakamoto, N., Matusoka, S., Ohishi, N., Yagi, K., Lipid peroxide level in plasma of diabetic patient (1979) Biochem. Med, 21, pp. 104-108; McCord, J.M., Fridovich, I., Superoxide dismutase: An enzymatic function for erythrocupreil (hemocuprein) (1969) J. Biol. Chem, 244, pp. 6049-6055; Czapski, G., Goldstein, S., Requirements for SOD mimics operating in vitro to work also in vivo (1991) Free. Radic. Res. Comm, 12-13, pp. 167-171; Omar, B.A., Flores, S.C., McCord, J.M., Superoxide dismutase: Pharmacological developments and applications (1992) Adv. Pharmacol, 23, pp. 109-161; Mates, J.M., Effect of antioxidant enzymes in the molecular control of reactive oxygen species toxicology (2000) Toxicology, 153, pp. 83-104; Riley, D.P., Functional mimics of superoxide dismutase enzymes as therapeutic agents (1999) Chem. Rev, 99, pp. 2573-2587; Barik, A., Mishra, B., Shen, L., Mohan, H., Kadam, R., Dutta, M., Hong-Yu Zhang, S., Priyadarsini, K.I., Evaluation of a new copper (II)-curcumin complex as superoxide dismutase mimic and its free radical reactions (2005) Free. Radic. Biol. Med, 39, pp. 811-822; Lowry, O.H., Rosenhrough, N.J., Farr, A.L., Randall, Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem, 193, pp. 270-272; Prabhakar, K.R., Veerapur, V.P., Vipan Parihar, K., Priyadarsini, K.I., Rao, B.S.S., Unnikrishnan, M.K., Evaluation and optimization of radioprotective activity of Coronopus didymus Linn. in gamma-irradiated mice (2006) Int. J. Radiat. Biol, 82, pp. 525-536; Gelvan, D., Saltman, P., Different cellular targets of Cu- and Fe-catalyzed oxidation observed using a Cu-compatible thiobarbiturate acid assay (1990) Biochim. Biophys. Acta, 1035, pp. 353-360; Karbownik, M., Reiter, R.J., Antioxidative effects of melatonin in protection against cellular damage caused by ionizing radiation (2000) Proceedings of the Society of Experimental Biology and Medicine, 225, pp. 9-22; Parasassi, T., Giusti, A.M., Gratton, E., Monaco, E., Raimondi, M., Ravagnan, G., Sapora, O., Evidence for an increase in water concentration in bilayers after oxidative damage of phospholipids induced by ionizing radiation (1994) Int. J. Radiat. Biol, 65, pp. 329-334; Navarro, J., Obrador, E., Pellicer, J.A., Aseni, M., Vina, J., Estrela, M., Blood glutathione as an index of radiation-induced oxidative stress in mice and humans (1997) Free. Radic. Biol. Med, 22, pp. 1203-1207; Susan, M., Rao, M.N.A., Interaction of glutathione with curcumin (1991) Int. J. Pharmaceutics, 76, pp. 257-259; Susan, M., Rao, M.N.A., Induction of Glutathione-S- transferease activity by curcumin in mice (1992) Arzneim.-Forsch./ Drug Res, 42 (II), pp. 962-964",
year = "2007",
doi = "10.1269/jrr.06103",
language = "English",
volume = "48",
pages = "241--245",
journal = "Journal of Radiation Research",
issn = "0449-3060",
publisher = "Japan Radiation Research Society",
number = "3",

}

Effect of curcumin and curcumin copper complex (1:1) on radiation-induced changes of anti-oxidant enzymes levels in the livers of swiss albino mice. / Koiram, P.R.; Veerapur, V.P.; Kunwar, A.; Mishra, B.; Barik, A.; Priyadarsini, I.K.; Mazhuvancherry, U.K.

In: Journal of Radiation Research, Vol. 48, No. 3, 2007, p. 241-245.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of curcumin and curcumin copper complex (1:1) on radiation-induced changes of anti-oxidant enzymes levels in the livers of swiss albino mice

AU - Koiram, P.R.

AU - Veerapur, V.P.

AU - Kunwar, A.

AU - Mishra, B.

AU - Barik, A.

AU - Priyadarsini, I.K.

AU - Mazhuvancherry, U.K.

N1 - Cited By :13 Export Date: 10 November 2017 Correspondence Address: Mazhuvancherry, U.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal - 576 104, India; email: mkunnikrishnan@gmail.com Chemicals/CAS: catalase, 9001-05-2; copper, 15158-11-9, 7440-50-8; curcumin, 458-37-7; glutathione transferase, 50812-37-8; Antioxidants; Catalase, 1.11.1.6; Copper, 7440-50-8; Curcumin, 458-37-7; Glutathione Transferase, 2.5.1.18; Radiation-Protective Agents References: Abrescia, P., Golino, P., Free radicals and antioxidants in cardiovascular diseases (2005) Expert. Rev. Cardiovasc. Ther, 3, pp. 159-171; Aggarwal, B.B., Kumar, A., Bharti, A.C., Anticancer potential of curcumin: Preclinical and clinical studies (2003) Anticancer Res, 23, pp. 363-398; Sharma, R.A., Gescher, A.J., Steward, W.P., Curcumin: The story so far (2005) Eur. J. Cancer, 41, pp. 1955-1968; Shishodia, S., Sethi, G., Aggarwal, B.B., Curcumin: Getting back to roots (2005) Ann. N.Y. Acad. Sci, 1056, pp. 206-217; Sato, Y., Hotta, N., Sakamoto, N., Matusoka, S., Ohishi, N., Yagi, K., Lipid peroxide level in plasma of diabetic patient (1979) Biochem. Med, 21, pp. 104-108; McCord, J.M., Fridovich, I., Superoxide dismutase: An enzymatic function for erythrocupreil (hemocuprein) (1969) J. Biol. Chem, 244, pp. 6049-6055; Czapski, G., Goldstein, S., Requirements for SOD mimics operating in vitro to work also in vivo (1991) Free. Radic. Res. Comm, 12-13, pp. 167-171; Omar, B.A., Flores, S.C., McCord, J.M., Superoxide dismutase: Pharmacological developments and applications (1992) Adv. Pharmacol, 23, pp. 109-161; Mates, J.M., Effect of antioxidant enzymes in the molecular control of reactive oxygen species toxicology (2000) Toxicology, 153, pp. 83-104; Riley, D.P., Functional mimics of superoxide dismutase enzymes as therapeutic agents (1999) Chem. Rev, 99, pp. 2573-2587; Barik, A., Mishra, B., Shen, L., Mohan, H., Kadam, R., Dutta, M., Hong-Yu Zhang, S., Priyadarsini, K.I., Evaluation of a new copper (II)-curcumin complex as superoxide dismutase mimic and its free radical reactions (2005) Free. Radic. Biol. Med, 39, pp. 811-822; Lowry, O.H., Rosenhrough, N.J., Farr, A.L., Randall, Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem, 193, pp. 270-272; Prabhakar, K.R., Veerapur, V.P., Vipan Parihar, K., Priyadarsini, K.I., Rao, B.S.S., Unnikrishnan, M.K., Evaluation and optimization of radioprotective activity of Coronopus didymus Linn. in gamma-irradiated mice (2006) Int. J. Radiat. Biol, 82, pp. 525-536; Gelvan, D., Saltman, P., Different cellular targets of Cu- and Fe-catalyzed oxidation observed using a Cu-compatible thiobarbiturate acid assay (1990) Biochim. Biophys. Acta, 1035, pp. 353-360; Karbownik, M., Reiter, R.J., Antioxidative effects of melatonin in protection against cellular damage caused by ionizing radiation (2000) Proceedings of the Society of Experimental Biology and Medicine, 225, pp. 9-22; Parasassi, T., Giusti, A.M., Gratton, E., Monaco, E., Raimondi, M., Ravagnan, G., Sapora, O., Evidence for an increase in water concentration in bilayers after oxidative damage of phospholipids induced by ionizing radiation (1994) Int. J. Radiat. Biol, 65, pp. 329-334; Navarro, J., Obrador, E., Pellicer, J.A., Aseni, M., Vina, J., Estrela, M., Blood glutathione as an index of radiation-induced oxidative stress in mice and humans (1997) Free. Radic. Biol. Med, 22, pp. 1203-1207; Susan, M., Rao, M.N.A., Interaction of glutathione with curcumin (1991) Int. J. Pharmaceutics, 76, pp. 257-259; Susan, M., Rao, M.N.A., Induction of Glutathione-S- transferease activity by curcumin in mice (1992) Arzneim.-Forsch./ Drug Res, 42 (II), pp. 962-964

PY - 2007

Y1 - 2007

N2 - Radiation Antioxidant enzymes Curcumin Curcumin copper complex GSH. The effect of mononuclear copper (II) complex of curcumin in 1:1 stoichiometry (hereafter referred to as complex) administered 30 min before γ-irradiation (4.5 Gy) on alterations in antioxidant and Thiobarbituric acid reactive substances (TBARS) levels in livers was studied in comparison to curcumin at a dose of 50 mg/kg. The different antioxidants like GSH, GST, catalase, SOD, TBARS and total thiols were estimated in the liver homogenates excised at different time intervals (1, 2 and 4 h) post irradiation using colorimetric methods. There was a radiation-induced decrease in the levels of all the studied enzymes at 1 h post irradiation, while an increase was observed at later time points. Both curcumin and complex treatment in sham-irradiated mice decreased the levels of GSH and total thiols, whereas there was an increase in the levels of catalase, GST and SOD compared to normal control. Under the influence of irradiation, both curcumin and complex treatment protected the decline in the levels of GSH, GST, SOD, catalase and total thiols, and inhibited radiation-induced lipid peroxidation. Further, the complex was found to be more effective in protecting the enzymes at 1 h post irradiation compared to curcumin treated group. This may be due to the higher rate constants of the complex compared to curcumin for their reactions with various free radicals.

AB - Radiation Antioxidant enzymes Curcumin Curcumin copper complex GSH. The effect of mononuclear copper (II) complex of curcumin in 1:1 stoichiometry (hereafter referred to as complex) administered 30 min before γ-irradiation (4.5 Gy) on alterations in antioxidant and Thiobarbituric acid reactive substances (TBARS) levels in livers was studied in comparison to curcumin at a dose of 50 mg/kg. The different antioxidants like GSH, GST, catalase, SOD, TBARS and total thiols were estimated in the liver homogenates excised at different time intervals (1, 2 and 4 h) post irradiation using colorimetric methods. There was a radiation-induced decrease in the levels of all the studied enzymes at 1 h post irradiation, while an increase was observed at later time points. Both curcumin and complex treatment in sham-irradiated mice decreased the levels of GSH and total thiols, whereas there was an increase in the levels of catalase, GST and SOD compared to normal control. Under the influence of irradiation, both curcumin and complex treatment protected the decline in the levels of GSH, GST, SOD, catalase and total thiols, and inhibited radiation-induced lipid peroxidation. Further, the complex was found to be more effective in protecting the enzymes at 1 h post irradiation compared to curcumin treated group. This may be due to the higher rate constants of the complex compared to curcumin for their reactions with various free radicals.

U2 - 10.1269/jrr.06103

DO - 10.1269/jrr.06103

M3 - Article

VL - 48

SP - 241

EP - 245

JO - Journal of Radiation Research

JF - Journal of Radiation Research

SN - 0449-3060

IS - 3

ER -