Effect of ethanolic extract of Cyanotis cristata leaves applied topically on wound healing in wistar rats

Anurag Pathak, Smita Shenoy, Sushil Kiran, Avinash Arivazahan, Deepak Nayak, Naveen Kumar, Amod Tilak, Manjunath Shetty

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: The objective of this study was to assess the effect of ethanolic extract of Cyanotis cristata leaves when applied topically on healing of wound in Wistar rats. Methods: C. cristata leaves were evaluated for its effect on healing of wound in three models, namely, incision, excision, and burn wound. Each model included five groups, each consisting of 6 rats. The five groups were as follows: Group I rats (control and ointment base), Group II rats (standard and silver sulfadiazine), and Group III, Group IV, Group V (treated with C. cristata extract ointment of 0.5%, 1%, and 2%, respectively). Variables evaluated included breaking strength in incision wound while it was contraction rate and epithelialization in excision and burn wound. One-way analysis of variance and Tukey’s post hoc test was used to analyze data. Result: In incision wound, breaking strength in test group was significantly increased (p<0.001) as compared to control. In excision and burn wound of test group, time to epithelialization and contraction rate was significantly decreased (p<0.001). The granulation tissue from excision and burn wound showed increased collagen and less inflammatory cells in test groups in comparison to the control. Conclusion: The ethanolic extract of leaves of C. cristata, when applied topically, enhanced wound strength and accelerated healing of incision, excision, and burn wounds in rats.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalAsian Journal of Pharmaceutical and Clinical Research
Volume10
Issue number12
DOIs
Publication statusPublished - 01-01-2017

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Wound Healing
Wistar Rats
Wounds and Injuries
Ointment Bases
Silver Sulfadiazine
Granulation Tissue
Ointments
Analysis of Variance
Collagen

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

Pathak, Anurag ; Shenoy, Smita ; Kiran, Sushil ; Arivazahan, Avinash ; Nayak, Deepak ; Kumar, Naveen ; Tilak, Amod ; Shetty, Manjunath. / Effect of ethanolic extract of Cyanotis cristata leaves applied topically on wound healing in wistar rats. In: Asian Journal of Pharmaceutical and Clinical Research. 2017 ; Vol. 10, No. 12. pp. 63-67.
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abstract = "Objective: The objective of this study was to assess the effect of ethanolic extract of Cyanotis cristata leaves when applied topically on healing of wound in Wistar rats. Methods: C. cristata leaves were evaluated for its effect on healing of wound in three models, namely, incision, excision, and burn wound. Each model included five groups, each consisting of 6 rats. The five groups were as follows: Group I rats (control and ointment base), Group II rats (standard and silver sulfadiazine), and Group III, Group IV, Group V (treated with C. cristata extract ointment of 0.5{\%}, 1{\%}, and 2{\%}, respectively). Variables evaluated included breaking strength in incision wound while it was contraction rate and epithelialization in excision and burn wound. One-way analysis of variance and Tukey’s post hoc test was used to analyze data. Result: In incision wound, breaking strength in test group was significantly increased (p<0.001) as compared to control. In excision and burn wound of test group, time to epithelialization and contraction rate was significantly decreased (p<0.001). The granulation tissue from excision and burn wound showed increased collagen and less inflammatory cells in test groups in comparison to the control. Conclusion: The ethanolic extract of leaves of C. cristata, when applied topically, enhanced wound strength and accelerated healing of incision, excision, and burn wounds in rats.",
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Effect of ethanolic extract of Cyanotis cristata leaves applied topically on wound healing in wistar rats. / Pathak, Anurag; Shenoy, Smita; Kiran, Sushil; Arivazahan, Avinash; Nayak, Deepak; Kumar, Naveen; Tilak, Amod; Shetty, Manjunath.

In: Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, No. 12, 01.01.2017, p. 63-67.

Research output: Contribution to journalArticle

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AU - Pathak, Anurag

AU - Shenoy, Smita

AU - Kiran, Sushil

AU - Arivazahan, Avinash

AU - Nayak, Deepak

AU - Kumar, Naveen

AU - Tilak, Amod

AU - Shetty, Manjunath

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N2 - Objective: The objective of this study was to assess the effect of ethanolic extract of Cyanotis cristata leaves when applied topically on healing of wound in Wistar rats. Methods: C. cristata leaves were evaluated for its effect on healing of wound in three models, namely, incision, excision, and burn wound. Each model included five groups, each consisting of 6 rats. The five groups were as follows: Group I rats (control and ointment base), Group II rats (standard and silver sulfadiazine), and Group III, Group IV, Group V (treated with C. cristata extract ointment of 0.5%, 1%, and 2%, respectively). Variables evaluated included breaking strength in incision wound while it was contraction rate and epithelialization in excision and burn wound. One-way analysis of variance and Tukey’s post hoc test was used to analyze data. Result: In incision wound, breaking strength in test group was significantly increased (p<0.001) as compared to control. In excision and burn wound of test group, time to epithelialization and contraction rate was significantly decreased (p<0.001). The granulation tissue from excision and burn wound showed increased collagen and less inflammatory cells in test groups in comparison to the control. Conclusion: The ethanolic extract of leaves of C. cristata, when applied topically, enhanced wound strength and accelerated healing of incision, excision, and burn wounds in rats.

AB - Objective: The objective of this study was to assess the effect of ethanolic extract of Cyanotis cristata leaves when applied topically on healing of wound in Wistar rats. Methods: C. cristata leaves were evaluated for its effect on healing of wound in three models, namely, incision, excision, and burn wound. Each model included five groups, each consisting of 6 rats. The five groups were as follows: Group I rats (control and ointment base), Group II rats (standard and silver sulfadiazine), and Group III, Group IV, Group V (treated with C. cristata extract ointment of 0.5%, 1%, and 2%, respectively). Variables evaluated included breaking strength in incision wound while it was contraction rate and epithelialization in excision and burn wound. One-way analysis of variance and Tukey’s post hoc test was used to analyze data. Result: In incision wound, breaking strength in test group was significantly increased (p<0.001) as compared to control. In excision and burn wound of test group, time to epithelialization and contraction rate was significantly decreased (p<0.001). The granulation tissue from excision and burn wound showed increased collagen and less inflammatory cells in test groups in comparison to the control. Conclusion: The ethanolic extract of leaves of C. cristata, when applied topically, enhanced wound strength and accelerated healing of incision, excision, and burn wounds in rats.

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