Effect of fluoride varnish and chlorhexidine-thymol varnish on mutans streptococci levels in human dental plaque: A double-blinded randomized controlled trial

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Abstract

Background: Recent systematic reviews on clinical trials comparing the efficacy of chlorhexidine and fluoride varnish found that the evidence was inconclusive and further well-conducted randomized controlled clinical trials were advocated. Aim: To compare the effect of fluoride varnish (F) and Chlorhexidine-thymol varnish (CHX/T) with intensive application regimen on mutans streptococci (MS) levels in human dental plaque. Design: Seventy-five subjects between 6 and 10 years of age were allocated into three groups: Group 1-F varnish (n = 29); Group 2-CHX/T varnish(n = 29); Group 3-placebo varnish (n = 17) by stratified block randomization. After baseline plaque samples were obtained, varnish application was applied and repeated at an interval of 3 days in each group. Plaque samples were repeated at 48 h, 1 month, and 3 months. The samples were spread over mitis-salivarius-bacitracin (MSB) culture media, and the colony-forming units per ml (CFU) were measured. Results: In both Groups 1 and 2, Wilcoxon matched-pairs signed-rank test revealed significant differences in log CFU values of MS between baseline and 48 h, baseline and 1 month but no significant difference between baseline and 3 months. An intergroup comparison at different time intervals showed that the difference between three groups was statistically insignificant. Conclusion: F varnish and CHX/T varnish, with an intensive regimen application have equivocal effect on MS levels in dental plaque.

Original languageEnglish
Pages (from-to)399-408
Number of pages10
JournalInternational Journal of Paediatric Dentistry
Volume24
Issue number6
DOIs
Publication statusPublished - 01-01-2014

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Topical Fluorides
Dental Plaque
Streptococcus mutans
Paint
Randomized Controlled Trials
Bacitracin
thymol drug combination chlorhexidine
Chlorhexidine
Random Allocation
Culture Media
Stem Cells
Placebos
Clinical Trials

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

@article{96bf07bb9aef41039c2c500b11dd5e86,
title = "Effect of fluoride varnish and chlorhexidine-thymol varnish on mutans streptococci levels in human dental plaque: A double-blinded randomized controlled trial",
abstract = "Background: Recent systematic reviews on clinical trials comparing the efficacy of chlorhexidine and fluoride varnish found that the evidence was inconclusive and further well-conducted randomized controlled clinical trials were advocated. Aim: To compare the effect of fluoride varnish (F) and Chlorhexidine-thymol varnish (CHX/T) with intensive application regimen on mutans streptococci (MS) levels in human dental plaque. Design: Seventy-five subjects between 6 and 10 years of age were allocated into three groups: Group 1-F varnish (n = 29); Group 2-CHX/T varnish(n = 29); Group 3-placebo varnish (n = 17) by stratified block randomization. After baseline plaque samples were obtained, varnish application was applied and repeated at an interval of 3 days in each group. Plaque samples were repeated at 48 h, 1 month, and 3 months. The samples were spread over mitis-salivarius-bacitracin (MSB) culture media, and the colony-forming units per ml (CFU) were measured. Results: In both Groups 1 and 2, Wilcoxon matched-pairs signed-rank test revealed significant differences in log CFU values of MS between baseline and 48 h, baseline and 1 month but no significant difference between baseline and 3 months. An intergroup comparison at different time intervals showed that the difference between three groups was statistically insignificant. Conclusion: F varnish and CHX/T varnish, with an intensive regimen application have equivocal effect on MS levels in dental plaque.",
author = "Sanchit Paul and {Baranya Shrikrishna}, Suprabha and Ethel Suman and Ramya Shenoy and Arathi Rao",
year = "2014",
month = "1",
day = "1",
doi = "10.1111/ipd.12085",
language = "English",
volume = "24",
pages = "399--408",
journal = "International Journal of Paediatric Dentistry",
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TY - JOUR

T1 - Effect of fluoride varnish and chlorhexidine-thymol varnish on mutans streptococci levels in human dental plaque

T2 - A double-blinded randomized controlled trial

AU - Paul, Sanchit

AU - Baranya Shrikrishna, Suprabha

AU - Suman, Ethel

AU - Shenoy, Ramya

AU - Rao, Arathi

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: Recent systematic reviews on clinical trials comparing the efficacy of chlorhexidine and fluoride varnish found that the evidence was inconclusive and further well-conducted randomized controlled clinical trials were advocated. Aim: To compare the effect of fluoride varnish (F) and Chlorhexidine-thymol varnish (CHX/T) with intensive application regimen on mutans streptococci (MS) levels in human dental plaque. Design: Seventy-five subjects between 6 and 10 years of age were allocated into three groups: Group 1-F varnish (n = 29); Group 2-CHX/T varnish(n = 29); Group 3-placebo varnish (n = 17) by stratified block randomization. After baseline plaque samples were obtained, varnish application was applied and repeated at an interval of 3 days in each group. Plaque samples were repeated at 48 h, 1 month, and 3 months. The samples were spread over mitis-salivarius-bacitracin (MSB) culture media, and the colony-forming units per ml (CFU) were measured. Results: In both Groups 1 and 2, Wilcoxon matched-pairs signed-rank test revealed significant differences in log CFU values of MS between baseline and 48 h, baseline and 1 month but no significant difference between baseline and 3 months. An intergroup comparison at different time intervals showed that the difference between three groups was statistically insignificant. Conclusion: F varnish and CHX/T varnish, with an intensive regimen application have equivocal effect on MS levels in dental plaque.

AB - Background: Recent systematic reviews on clinical trials comparing the efficacy of chlorhexidine and fluoride varnish found that the evidence was inconclusive and further well-conducted randomized controlled clinical trials were advocated. Aim: To compare the effect of fluoride varnish (F) and Chlorhexidine-thymol varnish (CHX/T) with intensive application regimen on mutans streptococci (MS) levels in human dental plaque. Design: Seventy-five subjects between 6 and 10 years of age were allocated into three groups: Group 1-F varnish (n = 29); Group 2-CHX/T varnish(n = 29); Group 3-placebo varnish (n = 17) by stratified block randomization. After baseline plaque samples were obtained, varnish application was applied and repeated at an interval of 3 days in each group. Plaque samples were repeated at 48 h, 1 month, and 3 months. The samples were spread over mitis-salivarius-bacitracin (MSB) culture media, and the colony-forming units per ml (CFU) were measured. Results: In both Groups 1 and 2, Wilcoxon matched-pairs signed-rank test revealed significant differences in log CFU values of MS between baseline and 48 h, baseline and 1 month but no significant difference between baseline and 3 months. An intergroup comparison at different time intervals showed that the difference between three groups was statistically insignificant. Conclusion: F varnish and CHX/T varnish, with an intensive regimen application have equivocal effect on MS levels in dental plaque.

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