Abstract
Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×105 to 1.9×105 after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×105 to 2.3×10 5 and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and 1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.
| Original language | English |
|---|---|
| Pages (from-to) | 147-152 |
| Number of pages | 6 |
| Journal | Radiation Physics and Chemistry |
| Volume | 78 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 01-02-2009 |
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All Science Journal Classification (ASJC) codes
- Radiation
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Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus. / Divyashree, M. S.; Shamala, T. R.
In: Radiation Physics and Chemistry, Vol. 78, No. 2, 01.02.2009, p. 147-152.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus
AU - Divyashree, M. S.
AU - Shamala, T. R.
PY - 2009/2/1
Y1 - 2009/2/1
N2 - Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×105 to 1.9×105 after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×105 to 2.3×10 5 and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and 1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.
AB - Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×105 to 1.9×105 after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×105 to 2.3×10 5 and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and 1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.
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UR - http://www.scopus.com/inward/citedby.url?scp=56349148315&partnerID=8YFLogxK
U2 - 10.1016/j.radphyschem.2008.08.010
DO - 10.1016/j.radphyschem.2008.08.010
M3 - Article
VL - 78
SP - 147
EP - 152
JO - Radiation Physics and Chemistry
JF - Radiation Physics and Chemistry
SN - 0969-806X
IS - 2
ER -