TY - JOUR
T1 - Effect of gamma irradiation on cell lysis and polyhydroxyalkanoate produced by Bacillus flexus
AU - Divyashree, M. S.
AU - Shamala, T. R.
PY - 2009/2/1
Y1 - 2009/2/1
N2 - Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×105 to 1.9×105 after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×105 to 2.3×10 5 and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and 1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.
AB - Bacillus flexus cultivated on sucrose and sucrose with plant oil such as castor oil produced polyhydroxybutyrate (PHB), a homopolymer of polyhydroxyalkanoate (PHA) and PHA copolymer (containing hydroxybutyrate and hexanoate), respectively. Gamma irradiation of these cells (5-40 kGy) resulted in cell damage and aided in the isolation of 45% and 54% PHA on biomass weight, correspondingly. Molecular weight of PHB increased from 1.5×105 to 1.9×105 after irradiation (10 kGy), with marginal increase of tensile strength from 18 to 20 MPa. At the same irradiation dosage, PHA copolymer showed higher molecular weight increase from 1.7×105 to 2.3×10 5 and tensile strength from 20 to 35 MPa. GC, GC-MS, FTIR and 1H NMR were used for the characterization of PHA. Gamma irradiation seems to be a novel technique, to induce cross-linking and molecular weight increase of PHA copolymer and aid in easy extractability of intracellular PHA, simultaneously.
UR - http://www.scopus.com/inward/record.url?scp=56349148315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56349148315&partnerID=8YFLogxK
U2 - 10.1016/j.radphyschem.2008.08.010
DO - 10.1016/j.radphyschem.2008.08.010
M3 - Article
AN - SCOPUS:56349148315
VL - 78
SP - 147
EP - 152
JO - Radiation Physics and Chemistry
JF - Radiation Physics and Chemistry
SN - 0969-806X
IS - 2
ER -