Effect of gamma radiation on the foetal haemopoietic system in the mouse

P. Uma Devi, M. Hossain, K. S. Bisht

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: To study the effect of gamma irradiation, in the late foetal period, on the haemopoietic progenitor cells of the mouse foetus. Materials and Methods: Pregnant Swiss mice were exposed to 0.1 to 1.5 Gy of Cobalt-60 gamma radiation on the 17th day of gestation. At 24h (18 day p.c.) and 72h (20 day p.c.) after exposure the weights of foetal liver and spleen and their mean cellularity were determined. Chromosomal aberrations and micronuclei (MN) were studied at 94 h. Haemopoietic progenitor cell survival at 24 and 72h post-irradiation was studied by exogenous spleen colony assay. Results: Irradiation caused significant reduction in the weights of foetal liver and spleen, which was more pronounced at 72h postirradiation. The mean cellularity as well as the CFU-S8 and CFU-S12 of liver and spleen showed a radiation dose-dependent decrease, but the spleen showed a higher sensitivity than liver to doses below 1.0Gy. Chromosomal damage in liver increased linearly with dose, while in spleen the increase was linear-quadratic. Conclusions: Tim results demonstrate the damaging effect of low doses of gamma radiation on the late foetal haemopoietic progenitor cells of the mouse. Induction of cytogenetic damage appears to be a no-threshold effect in the dose range used.

Original languageEnglish
Pages (from-to)639-646
Number of pages8
JournalInternational Journal of Radiation Biology
Volume74
Issue number5
Publication statusPublished - 07-12-1998
Externally publishedYes

Fingerprint

Gamma Rays
Spleen
Liver
Fetal Weight
Stem Cells
Cobalt
Cytogenetics
Chromosome Aberrations
Weight Loss
Cell Survival
Fetus
Radiation
Pregnancy

All Science Journal Classification (ASJC) codes

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

Uma Devi, P. ; Hossain, M. ; Bisht, K. S. / Effect of gamma radiation on the foetal haemopoietic system in the mouse. In: International Journal of Radiation Biology. 1998 ; Vol. 74, No. 5. pp. 639-646.
@article{1bdbaed1d7204bcf9d44942e070afbc1,
title = "Effect of gamma radiation on the foetal haemopoietic system in the mouse",
abstract = "Purpose: To study the effect of gamma irradiation, in the late foetal period, on the haemopoietic progenitor cells of the mouse foetus. Materials and Methods: Pregnant Swiss mice were exposed to 0.1 to 1.5 Gy of Cobalt-60 gamma radiation on the 17th day of gestation. At 24h (18 day p.c.) and 72h (20 day p.c.) after exposure the weights of foetal liver and spleen and their mean cellularity were determined. Chromosomal aberrations and micronuclei (MN) were studied at 94 h. Haemopoietic progenitor cell survival at 24 and 72h post-irradiation was studied by exogenous spleen colony assay. Results: Irradiation caused significant reduction in the weights of foetal liver and spleen, which was more pronounced at 72h postirradiation. The mean cellularity as well as the CFU-S8 and CFU-S12 of liver and spleen showed a radiation dose-dependent decrease, but the spleen showed a higher sensitivity than liver to doses below 1.0Gy. Chromosomal damage in liver increased linearly with dose, while in spleen the increase was linear-quadratic. Conclusions: Tim results demonstrate the damaging effect of low doses of gamma radiation on the late foetal haemopoietic progenitor cells of the mouse. Induction of cytogenetic damage appears to be a no-threshold effect in the dose range used.",
author = "{Uma Devi}, P. and M. Hossain and Bisht, {K. S.}",
year = "1998",
month = "12",
day = "7",
language = "English",
volume = "74",
pages = "639--646",
journal = "International Journal of Radiation Biology",
issn = "0955-3002",
publisher = "Informa Healthcare",
number = "5",

}

Uma Devi, P, Hossain, M & Bisht, KS 1998, 'Effect of gamma radiation on the foetal haemopoietic system in the mouse', International Journal of Radiation Biology, vol. 74, no. 5, pp. 639-646.

Effect of gamma radiation on the foetal haemopoietic system in the mouse. / Uma Devi, P.; Hossain, M.; Bisht, K. S.

In: International Journal of Radiation Biology, Vol. 74, No. 5, 07.12.1998, p. 639-646.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of gamma radiation on the foetal haemopoietic system in the mouse

AU - Uma Devi, P.

AU - Hossain, M.

AU - Bisht, K. S.

PY - 1998/12/7

Y1 - 1998/12/7

N2 - Purpose: To study the effect of gamma irradiation, in the late foetal period, on the haemopoietic progenitor cells of the mouse foetus. Materials and Methods: Pregnant Swiss mice were exposed to 0.1 to 1.5 Gy of Cobalt-60 gamma radiation on the 17th day of gestation. At 24h (18 day p.c.) and 72h (20 day p.c.) after exposure the weights of foetal liver and spleen and their mean cellularity were determined. Chromosomal aberrations and micronuclei (MN) were studied at 94 h. Haemopoietic progenitor cell survival at 24 and 72h post-irradiation was studied by exogenous spleen colony assay. Results: Irradiation caused significant reduction in the weights of foetal liver and spleen, which was more pronounced at 72h postirradiation. The mean cellularity as well as the CFU-S8 and CFU-S12 of liver and spleen showed a radiation dose-dependent decrease, but the spleen showed a higher sensitivity than liver to doses below 1.0Gy. Chromosomal damage in liver increased linearly with dose, while in spleen the increase was linear-quadratic. Conclusions: Tim results demonstrate the damaging effect of low doses of gamma radiation on the late foetal haemopoietic progenitor cells of the mouse. Induction of cytogenetic damage appears to be a no-threshold effect in the dose range used.

AB - Purpose: To study the effect of gamma irradiation, in the late foetal period, on the haemopoietic progenitor cells of the mouse foetus. Materials and Methods: Pregnant Swiss mice were exposed to 0.1 to 1.5 Gy of Cobalt-60 gamma radiation on the 17th day of gestation. At 24h (18 day p.c.) and 72h (20 day p.c.) after exposure the weights of foetal liver and spleen and their mean cellularity were determined. Chromosomal aberrations and micronuclei (MN) were studied at 94 h. Haemopoietic progenitor cell survival at 24 and 72h post-irradiation was studied by exogenous spleen colony assay. Results: Irradiation caused significant reduction in the weights of foetal liver and spleen, which was more pronounced at 72h postirradiation. The mean cellularity as well as the CFU-S8 and CFU-S12 of liver and spleen showed a radiation dose-dependent decrease, but the spleen showed a higher sensitivity than liver to doses below 1.0Gy. Chromosomal damage in liver increased linearly with dose, while in spleen the increase was linear-quadratic. Conclusions: Tim results demonstrate the damaging effect of low doses of gamma radiation on the late foetal haemopoietic progenitor cells of the mouse. Induction of cytogenetic damage appears to be a no-threshold effect in the dose range used.

UR - http://www.scopus.com/inward/record.url?scp=0031768239&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031768239&partnerID=8YFLogxK

M3 - Article

C2 - 9848283

AN - SCOPUS:0031768239

VL - 74

SP - 639

EP - 646

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

SN - 0955-3002

IS - 5

ER -