Effect of Garuga pinnata Roxb aqueous bark extract on tissue antioxidant activity in streptozotocin-nicotinamide-induced type II diabetic rats

A. Shirwaikar, K. Rajendran, R. Barik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Oxidative stress plays an important role in chronic complications of diabetes mellitus. In the current study, the antioxidant effect of oral administration of aqueous bark extract of Garuga pinnata Roxb. (Burseraceae) on tissue antioxidant enzymes and lipid peroxidation in liver and kidney of streptozotocin-nicotinamide-induced type II diabetic rats was evaluated. The diabetic rats showed lower activities of superoxide dismutase (SOD), glutathione peroxidase, glutathione transferase, and reduced glutathione content in liver and kidney, which were restored to near normal levels by treatment with the bark extract. The increased levels of lipid peroxidation (TBARS) in diabetic rats were reverted back to near normal levels after treatment with the bark extract. A two dose level study was conducted. The current study reveals the efficacy of Garuga pinnata aqueous bark extract in the amelioration of diabetes, which may be attributed to its antioxidant potential. © 2007 Informa Healthcare.
Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalPharmaceutical Biology
Volume45
Issue number3
DOIs
Publication statusPublished - 2007
Externally publishedYes

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Tissue Extracts
Niacinamide
Streptozocin
Antioxidants
Lipid Peroxidation
Burseraceae
Kidney
Liver
Diabetes Complications
Glutathione Peroxidase
Glutathione Transferase
Superoxide Dismutase
Glutathione
Oral Administration
Oxidative Stress
Delivery of Health Care
Enzymes
Therapeutics

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title = "Effect of Garuga pinnata Roxb aqueous bark extract on tissue antioxidant activity in streptozotocin-nicotinamide-induced type II diabetic rats",
abstract = "Oxidative stress plays an important role in chronic complications of diabetes mellitus. In the current study, the antioxidant effect of oral administration of aqueous bark extract of Garuga pinnata Roxb. (Burseraceae) on tissue antioxidant enzymes and lipid peroxidation in liver and kidney of streptozotocin-nicotinamide-induced type II diabetic rats was evaluated. The diabetic rats showed lower activities of superoxide dismutase (SOD), glutathione peroxidase, glutathione transferase, and reduced glutathione content in liver and kidney, which were restored to near normal levels by treatment with the bark extract. The increased levels of lipid peroxidation (TBARS) in diabetic rats were reverted back to near normal levels after treatment with the bark extract. A two dose level study was conducted. The current study reveals the efficacy of Garuga pinnata aqueous bark extract in the amelioration of diabetes, which may be attributed to its antioxidant potential. {\circledC} 2007 Informa Healthcare.",
author = "A. Shirwaikar and K. Rajendran and R. Barik",
note = "Cited By :2 Export Date: 10 November 2017 CODEN: PHBIF Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 576104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: glibenclamide, 10238-21-8; glutathione, 70-18-8; glutathione peroxidase, 9013-66-5; glutathione transferase, 50812-37-8; nicotinamide, 11032-50-1, 98-92-0; streptozocin, 18883-66-4; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1 References: Anuradha, C.V., Selvam, R., Effect of oral methionine on tissue lipid peroxidation and antioxidants in alloxan induced diabetic rats (1993) J Nutr Biochem, 4, pp. 212-216; Baynes, W.J., Role of oxidative stress in development of complications in diabetes (1991) Diabetes, 40, pp. 405-412; Bruce, A., Freeman, D., James, C., Free radicals and tissue injury (1982) Lab Invest, 47, pp. 412-416; Burk, R.F., Glutathione dependent protection by rat liver microsomal protein against lipid peroxidation (1983) Biochem Biophys Acta, 757, pp. 21-28; Efrat, S., Prospects for treatment of type 2 diabetes by expansion of the β-cell mass (2001) Diabetes, 50, pp. S189-S190; Giugliano, D., Ceriello, A., Paolisso, G., Oxidative stress and diabetic vascular complications (1996) Diabetes Care, 193, pp. 257-267; Griesmacher, A., Kinder, H.M., Andert, S., Enhanced serum levels of thiobarbituric acid reactive substances in diabetes mellitus (1995) Am J Med, 16, pp. 331-335; Ghosh, M.N., Fundamentals of experimental pharmacology (1984) Toxicity Studies, pp. 153-160. , Scientific Book Agency, Calcutta, pp; Haliwell, B., Gutteridge, J.M.C., Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy (1994) Lancet, 1, pp. 1396-1397; Harborne, J.B., (1998) Phytochemical Methods, pp. 60-63. , London, Chapman & Hall, pp; Jain, S.K., Sinha, B.K., Gupta, R.C., (1991) Notable Plants in Ethnomedicine of India, pp. 121-123. , Deep Publications, New Delhi, pp; Kapolwitz, N., Ookhtens, M., Aw, T.Y., The regulation of hepatic glutathione (1985) Ann Rev Pharmacol Toxicol, 25, pp. 715-744; Kasi, R., Balasubramanian, R., Sorimuthu, S., Protective effect of Eugenia jambolana seed kernel extract on tissue antioxidants in streptozotocin-induced diabetic rats (2004) Bio Pharm Bull, 27, pp. 1212-1217; Kirtikar KR, Basu BD (1991): Indian medicinal plants, In: Burseraceqe, Blatter E, Caivs JJ, Mhaskar KS, Periodical Experts Book Agency, 2nd ed. I, Periodical Experts Book Agency, New Delhi, pp. 524-525; Kokate, C.K., (1994) Practical Pharmacognosy, pp. 107-110. , New Delhi, Vallabh Prakashan, pp; Larson, R.A., The antioxidants of higher plants (1988) Phytochemistry, 27, pp. 969-978; Matkovics, B., Kotorman, M., Varga, I.S., QuyHai, D.Q., Varga, C., Oxidative stress in experimental diabetes induced by streptozotocin (1998) Acta Physiol Hung, 85, pp. 29-38; McCord, J.M., Keele, B.B., Fridovich, I., An enzyme based theory of obligate anaerobis; The physiological functions of superoxide dismutase (1976) Proc Natl Acad Sci USA, 68, pp. 1024-1031; McLennan, S.V., Heffernan, S., Wright, L., Rae, C., Fisher, E., Yue, D.K., Turtle, J.R., Changes in hepatic glutathione metabolism in diabetes (1991) Diabetes, 40, pp. 344-348; Meister, A., Anderson, M.E., Glutathione (1983) Annu Rev Biochem, 52, pp. 711-760; Menon, P., Viswanathan, P., Rukkumani, R., Sribalasubhashini, M., Ferulic acid alleviates lipid peroxidation in diabetic rats (2004) Phytother Res, 18, pp. 310-314; Misra, H.P., Fridovich, I., The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase (1972) J Biol Chem, 247, pp. 3170-3175; Nadkarni, A.K., (1954) Indian Material Medica, pp. 570-573. , Hindustan publishing Co, Mumbai, pp; Necheles, T.F., Boles, T.A., Allep, D.M., Erythrocyte glutathione peroxidase deficiency and hemolytic disease of the new born infant (1968) Journal of Pediatrics, 72, pp. 319-324; Okhawa, H., Ohishi, N., Yagi, K., Assay of lipid eroxides in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 95, pp. 351-158; Park, K., (2000) Parks Text Book of Preventive and Social Medicine, p. 292. , Jabalpur, M/s Bhanarsidas Bhanot Publications, p; Pellegrino, M., Christophe, B., Rene, G., Michele, R., Michele, M., Dominique, H.B., Michela, N., Gerard, R., Development of a new model of type II diabetes in adult rats administered with streptozotocin and nicotinamide (1998) Diabetes, 47, pp. 224-231; Rajsekaran, S., Sivagnanam, K., Subramanian, S., Modulatory effects of Aloe vera leaf gel extract on oxidative stress in rats treated with streptozotocin (2005) J Pharm Pharmacol, 57, pp. 241-246; Sedlak, J., Lindsay, R.H.L., Estimation of total and nonprotein sulfhydryl groups I tissue with Ellman's reagent (1968) Anal Biochem, 25, pp. 192-205; Sharma, A., Kharb, S., Chugh, S.N., Kakkar, R., Singh, G.P., Evaluation of oxidative stress before and after control of glycemia and after vitamin E supplementation in diabetic patients (2000) Metabolism, 49, pp. 160-162; The Wealth of India (1950): Garuga. In: A Dictionary of Indian Raw Materials and Industrial Products. New Delhi, CSIR, pp. 117-120; Turner, M.A., (1965) Screening Methods in Pharmacology, pp. 26-30. , NewYork, Academic Press, pp; Wohaeib, A., Godin, V.D., Alterations in free radical tissue-defense mechanisms in streptozotocin-induced diabetes in rat (1987) Diabetes, 36, pp. 1014-1018; Wolf, S.P., Dean, R.T., Glucose autooxidation and protein modification. The potential role of autoxidative glycosylation in diabetes mellitus (1987) J Biochem, 245, pp. 243-250; Wongsinkongman, P., Brossi, A., Wang, H.K., Bastow, K.F., Lee, K.H., Antitumour agents. Part 209: Pheophorbide a derivatives as photo independent cytotoxic agents (2002) Bioorg Med Chem, 10, pp. 583-591",
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doi = "10.1080/13880200701213120",
language = "English",
volume = "45",
pages = "205--209",
journal = "Pharmaceutical Biology",
issn = "1388-0209",
publisher = "Informa Healthcare",
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}

Effect of Garuga pinnata Roxb aqueous bark extract on tissue antioxidant activity in streptozotocin-nicotinamide-induced type II diabetic rats. / Shirwaikar, A.; Rajendran, K.; Barik, R.

In: Pharmaceutical Biology, Vol. 45, No. 3, 2007, p. 205-209.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of Garuga pinnata Roxb aqueous bark extract on tissue antioxidant activity in streptozotocin-nicotinamide-induced type II diabetic rats

AU - Shirwaikar, A.

AU - Rajendran, K.

AU - Barik, R.

N1 - Cited By :2 Export Date: 10 November 2017 CODEN: PHBIF Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 576104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: glibenclamide, 10238-21-8; glutathione, 70-18-8; glutathione peroxidase, 9013-66-5; glutathione transferase, 50812-37-8; nicotinamide, 11032-50-1, 98-92-0; streptozocin, 18883-66-4; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1 References: Anuradha, C.V., Selvam, R., Effect of oral methionine on tissue lipid peroxidation and antioxidants in alloxan induced diabetic rats (1993) J Nutr Biochem, 4, pp. 212-216; Baynes, W.J., Role of oxidative stress in development of complications in diabetes (1991) Diabetes, 40, pp. 405-412; Bruce, A., Freeman, D., James, C., Free radicals and tissue injury (1982) Lab Invest, 47, pp. 412-416; Burk, R.F., Glutathione dependent protection by rat liver microsomal protein against lipid peroxidation (1983) Biochem Biophys Acta, 757, pp. 21-28; Efrat, S., Prospects for treatment of type 2 diabetes by expansion of the β-cell mass (2001) Diabetes, 50, pp. S189-S190; Giugliano, D., Ceriello, A., Paolisso, G., Oxidative stress and diabetic vascular complications (1996) Diabetes Care, 193, pp. 257-267; Griesmacher, A., Kinder, H.M., Andert, S., Enhanced serum levels of thiobarbituric acid reactive substances in diabetes mellitus (1995) Am J Med, 16, pp. 331-335; Ghosh, M.N., Fundamentals of experimental pharmacology (1984) Toxicity Studies, pp. 153-160. , Scientific Book Agency, Calcutta, pp; Haliwell, B., Gutteridge, J.M.C., Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy (1994) Lancet, 1, pp. 1396-1397; Harborne, J.B., (1998) Phytochemical Methods, pp. 60-63. , London, Chapman & Hall, pp; Jain, S.K., Sinha, B.K., Gupta, R.C., (1991) Notable Plants in Ethnomedicine of India, pp. 121-123. , Deep Publications, New Delhi, pp; Kapolwitz, N., Ookhtens, M., Aw, T.Y., The regulation of hepatic glutathione (1985) Ann Rev Pharmacol Toxicol, 25, pp. 715-744; Kasi, R., Balasubramanian, R., Sorimuthu, S., Protective effect of Eugenia jambolana seed kernel extract on tissue antioxidants in streptozotocin-induced diabetic rats (2004) Bio Pharm Bull, 27, pp. 1212-1217; Kirtikar KR, Basu BD (1991): Indian medicinal plants, In: Burseraceqe, Blatter E, Caivs JJ, Mhaskar KS, Periodical Experts Book Agency, 2nd ed. I, Periodical Experts Book Agency, New Delhi, pp. 524-525; Kokate, C.K., (1994) Practical Pharmacognosy, pp. 107-110. , New Delhi, Vallabh Prakashan, pp; Larson, R.A., The antioxidants of higher plants (1988) Phytochemistry, 27, pp. 969-978; Matkovics, B., Kotorman, M., Varga, I.S., QuyHai, D.Q., Varga, C., Oxidative stress in experimental diabetes induced by streptozotocin (1998) Acta Physiol Hung, 85, pp. 29-38; McCord, J.M., Keele, B.B., Fridovich, I., An enzyme based theory of obligate anaerobis; The physiological functions of superoxide dismutase (1976) Proc Natl Acad Sci USA, 68, pp. 1024-1031; McLennan, S.V., Heffernan, S., Wright, L., Rae, C., Fisher, E., Yue, D.K., Turtle, J.R., Changes in hepatic glutathione metabolism in diabetes (1991) Diabetes, 40, pp. 344-348; Meister, A., Anderson, M.E., Glutathione (1983) Annu Rev Biochem, 52, pp. 711-760; Menon, P., Viswanathan, P., Rukkumani, R., Sribalasubhashini, M., Ferulic acid alleviates lipid peroxidation in diabetic rats (2004) Phytother Res, 18, pp. 310-314; Misra, H.P., Fridovich, I., The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase (1972) J Biol Chem, 247, pp. 3170-3175; Nadkarni, A.K., (1954) Indian Material Medica, pp. 570-573. , Hindustan publishing Co, Mumbai, pp; Necheles, T.F., Boles, T.A., Allep, D.M., Erythrocyte glutathione peroxidase deficiency and hemolytic disease of the new born infant (1968) Journal of Pediatrics, 72, pp. 319-324; Okhawa, H., Ohishi, N., Yagi, K., Assay of lipid eroxides in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 95, pp. 351-158; Park, K., (2000) Parks Text Book of Preventive and Social Medicine, p. 292. , Jabalpur, M/s Bhanarsidas Bhanot Publications, p; Pellegrino, M., Christophe, B., Rene, G., Michele, R., Michele, M., Dominique, H.B., Michela, N., Gerard, R., Development of a new model of type II diabetes in adult rats administered with streptozotocin and nicotinamide (1998) Diabetes, 47, pp. 224-231; Rajsekaran, S., Sivagnanam, K., Subramanian, S., Modulatory effects of Aloe vera leaf gel extract on oxidative stress in rats treated with streptozotocin (2005) J Pharm Pharmacol, 57, pp. 241-246; Sedlak, J., Lindsay, R.H.L., Estimation of total and nonprotein sulfhydryl groups I tissue with Ellman's reagent (1968) Anal Biochem, 25, pp. 192-205; Sharma, A., Kharb, S., Chugh, S.N., Kakkar, R., Singh, G.P., Evaluation of oxidative stress before and after control of glycemia and after vitamin E supplementation in diabetic patients (2000) Metabolism, 49, pp. 160-162; The Wealth of India (1950): Garuga. In: A Dictionary of Indian Raw Materials and Industrial Products. New Delhi, CSIR, pp. 117-120; Turner, M.A., (1965) Screening Methods in Pharmacology, pp. 26-30. , NewYork, Academic Press, pp; Wohaeib, A., Godin, V.D., Alterations in free radical tissue-defense mechanisms in streptozotocin-induced diabetes in rat (1987) Diabetes, 36, pp. 1014-1018; Wolf, S.P., Dean, R.T., Glucose autooxidation and protein modification. The potential role of autoxidative glycosylation in diabetes mellitus (1987) J Biochem, 245, pp. 243-250; Wongsinkongman, P., Brossi, A., Wang, H.K., Bastow, K.F., Lee, K.H., Antitumour agents. Part 209: Pheophorbide a derivatives as photo independent cytotoxic agents (2002) Bioorg Med Chem, 10, pp. 583-591

PY - 2007

Y1 - 2007

N2 - Oxidative stress plays an important role in chronic complications of diabetes mellitus. In the current study, the antioxidant effect of oral administration of aqueous bark extract of Garuga pinnata Roxb. (Burseraceae) on tissue antioxidant enzymes and lipid peroxidation in liver and kidney of streptozotocin-nicotinamide-induced type II diabetic rats was evaluated. The diabetic rats showed lower activities of superoxide dismutase (SOD), glutathione peroxidase, glutathione transferase, and reduced glutathione content in liver and kidney, which were restored to near normal levels by treatment with the bark extract. The increased levels of lipid peroxidation (TBARS) in diabetic rats were reverted back to near normal levels after treatment with the bark extract. A two dose level study was conducted. The current study reveals the efficacy of Garuga pinnata aqueous bark extract in the amelioration of diabetes, which may be attributed to its antioxidant potential. © 2007 Informa Healthcare.

AB - Oxidative stress plays an important role in chronic complications of diabetes mellitus. In the current study, the antioxidant effect of oral administration of aqueous bark extract of Garuga pinnata Roxb. (Burseraceae) on tissue antioxidant enzymes and lipid peroxidation in liver and kidney of streptozotocin-nicotinamide-induced type II diabetic rats was evaluated. The diabetic rats showed lower activities of superoxide dismutase (SOD), glutathione peroxidase, glutathione transferase, and reduced glutathione content in liver and kidney, which were restored to near normal levels by treatment with the bark extract. The increased levels of lipid peroxidation (TBARS) in diabetic rats were reverted back to near normal levels after treatment with the bark extract. A two dose level study was conducted. The current study reveals the efficacy of Garuga pinnata aqueous bark extract in the amelioration of diabetes, which may be attributed to its antioxidant potential. © 2007 Informa Healthcare.

U2 - 10.1080/13880200701213120

DO - 10.1080/13880200701213120

M3 - Article

VL - 45

SP - 205

EP - 209

JO - Pharmaceutical Biology

JF - Pharmaceutical Biology

SN - 1388-0209

IS - 3

ER -