Effect of intracerebroventricular methotrexate on brain amines

S. Madhyastha, S. N. Somayaji, M. S. Rao, K. Nalini, K. L. Bairy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Intrathecal methotrexate in children with leukemia is known to cause seizures, dementia, leukoencephalopathy and cognitive dysfunction. To investigate the role of brain amines in cognitive dysfunction, male Wistar rats were given multiple intracerebroventricular injections of methotrexate. Our earlier studies in this regard revealed disruption of brain monoamines in hippocampus with severe cytotoxic effect on CA4 hippocampal neurons. Further extending this study, the levels of brain monoamines in frontal cortex, hypothalamus and brainstem were estimated by HPLC method and histopathological study of the frontal cortex. The concentration of all three-brain amine (norepinephrine, dopamine and serotonin) levels was reduced in 2 mg/kg dose of methotrexate in frontal cortex and brain stem. Hypothalamus did not show any significant change in brain monoamine levels. No structural changes in the frontal cortex neurons were observed. Disruption of brain monoamines has been proposed as a cause of brain dysfunction from this chemotherapy. The outcome of the study may have therapeutic implications in the management of childhood lymphoblastic leukemia.

Original languageEnglish
Pages (from-to)427-435
Number of pages9
JournalIndian Journal of Physiology and Pharmacology
Volume49
Issue number4
Publication statusPublished - 10-2005

Fingerprint

Methotrexate
Amines
Frontal Lobe
Brain
Hypothalamus
Brain Stem
Leukoencephalopathies
Neurons
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Dementia
Wistar Rats
Hippocampus
Dopamine
Serotonin
Norepinephrine
Leukemia
Seizures
High Pressure Liquid Chromatography
Outcome Assessment (Health Care)
Drug Therapy

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology

Cite this

Madhyastha, S., Somayaji, S. N., Rao, M. S., Nalini, K., & Bairy, K. L. (2005). Effect of intracerebroventricular methotrexate on brain amines. Indian Journal of Physiology and Pharmacology, 49(4), 427-435.
Madhyastha, S. ; Somayaji, S. N. ; Rao, M. S. ; Nalini, K. ; Bairy, K. L. / Effect of intracerebroventricular methotrexate on brain amines. In: Indian Journal of Physiology and Pharmacology. 2005 ; Vol. 49, No. 4. pp. 427-435.
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Madhyastha, S, Somayaji, SN, Rao, MS, Nalini, K & Bairy, KL 2005, 'Effect of intracerebroventricular methotrexate on brain amines', Indian Journal of Physiology and Pharmacology, vol. 49, no. 4, pp. 427-435.

Effect of intracerebroventricular methotrexate on brain amines. / Madhyastha, S.; Somayaji, S. N.; Rao, M. S.; Nalini, K.; Bairy, K. L.

In: Indian Journal of Physiology and Pharmacology, Vol. 49, No. 4, 10.2005, p. 427-435.

Research output: Contribution to journalArticle

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Madhyastha S, Somayaji SN, Rao MS, Nalini K, Bairy KL. Effect of intracerebroventricular methotrexate on brain amines. Indian Journal of Physiology and Pharmacology. 2005 Oct;49(4):427-435.