Effect of lecithin on d-galactosamine induced hepatotoxicity through mitochondrial pathway involving Bcl-2 and Bax

P. Vasanth Raj, K. Nitesh, Jain Prateek, M. Neena Sankhe, J. Venkata Rao, C. Mallikarjuna Rao, N. Udupa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Twenty four Wistar strain albino rats were used for the investigations. Lecithin 50 and 100 mg/kg b wt was administered for 1 week by oral route. Liver damage was induced by intra peritoneal administration of 400 mg/kg b wt d-galactosamine on the last day. At the end of the study animals were sacrificed and liver enzyme levels, histopathology, mitochondrial integrity, expression of p53, Bax and Bcl-2 mRNA levels were studied. Increases in the liver enzyme levels by d-GalN were significantly inhibited by pretreatment with lecithin. Histopathological observation further confirmed the hepatoprotective effect of lecithin. In addition, the disruption of mitochondrial membrane, up regulation of Bax and down regulation of Bcl-2 mRNA levels in the liver of d-GalN intoxicated rats were effectively prevented by pretreatment with lecithin. The results of the present study validate our conviction that d-GalN causes hepatic damage via mitochondrial pathway involving Bax and Bcl-2.

Original languageEnglish
Pages (from-to)378-384
Number of pages7
JournalIndian Journal of Clinical Biochemistry
Volume26
Issue number4
DOIs
Publication statusPublished - 10-2011

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Galactosamine
Lecithins
Liver
Rats
Messenger RNA
Enzymes
Mitochondrial Membranes
Animals
Membranes
Up-Regulation
Down-Regulation
Observation

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

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title = "Effect of lecithin on d-galactosamine induced hepatotoxicity through mitochondrial pathway involving Bcl-2 and Bax",
abstract = "Twenty four Wistar strain albino rats were used for the investigations. Lecithin 50 and 100 mg/kg b wt was administered for 1 week by oral route. Liver damage was induced by intra peritoneal administration of 400 mg/kg b wt d-galactosamine on the last day. At the end of the study animals were sacrificed and liver enzyme levels, histopathology, mitochondrial integrity, expression of p53, Bax and Bcl-2 mRNA levels were studied. Increases in the liver enzyme levels by d-GalN were significantly inhibited by pretreatment with lecithin. Histopathological observation further confirmed the hepatoprotective effect of lecithin. In addition, the disruption of mitochondrial membrane, up regulation of Bax and down regulation of Bcl-2 mRNA levels in the liver of d-GalN intoxicated rats were effectively prevented by pretreatment with lecithin. The results of the present study validate our conviction that d-GalN causes hepatic damage via mitochondrial pathway involving Bax and Bcl-2.",
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Effect of lecithin on d-galactosamine induced hepatotoxicity through mitochondrial pathway involving Bcl-2 and Bax. / Raj, P. Vasanth; Nitesh, K.; Prateek, Jain; Sankhe, M. Neena; Rao, J. Venkata; Rao, C. Mallikarjuna; Udupa, N.

In: Indian Journal of Clinical Biochemistry, Vol. 26, No. 4, 10.2011, p. 378-384.

Research output: Contribution to journalArticle

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AU - Raj, P. Vasanth

AU - Nitesh, K.

AU - Prateek, Jain

AU - Sankhe, M. Neena

AU - Rao, J. Venkata

AU - Rao, C. Mallikarjuna

AU - Udupa, N.

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