Effect of methotrexate on AgNOR count in liver of wistar rats

Deepthinath Raghunathan, Laxminarayana Bairy Kurady, Ramachandra Bhat

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A study was undertaken to evaluate the Nucleolar organizing regions (NOR) count and histopathological changes in rat's liver treated with methotrexate (MTX). Male Wistar rats aged 4 months, maintained in our institution were used in the present study. Animals were injected intraperitonealy with 3 different doses of methotrexate on alternative days for one week and the animals were sacrificed on the seventh day by using ether. Paraffin slides were prepared and histopathology as well as AgNOR studies were done. In the histopathological study, liver showed disruption of normal hepatic plates, vaculation of the hepatocytes, nuclear variability and enlarged blood vessels. In AgNOR study, data showed an overall decrease in the total count (numerical) from normal to 12 mg dose MTX. The total count includes small, medium and large sized AgNORs. A statistically high significant difference was found from normal to 12 mg dose MTX as well as between the doses. In conclusion, the genotoxicity in rats is well documented with high dose of methotrexate (12 mg/kg) compared to low dose (8 mg/kg). Methotrexate treatment reduces the transcription rate and ploidy. Methotrexate reduces the cell proliferation. Thus it reduces the count.

Original languageEnglish
Pages (from-to)11-15
Number of pages5
JournalIranian Journal of Pharmacology and Therapeutics
Volume8
Issue number1
Publication statusPublished - 01-01-2009

Fingerprint

Methotrexate
Wistar Rats
Liver
Ploidies
nucleolar organizer region associated proteins
Ether
Paraffin
Blood Vessels
Hepatocytes
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Raghunathan, Deepthinath ; Kurady, Laxminarayana Bairy ; Bhat, Ramachandra. / Effect of methotrexate on AgNOR count in liver of wistar rats. In: Iranian Journal of Pharmacology and Therapeutics. 2009 ; Vol. 8, No. 1. pp. 11-15.
@article{2608719c4b9248118ae5d7921acfd647,
title = "Effect of methotrexate on AgNOR count in liver of wistar rats",
abstract = "A study was undertaken to evaluate the Nucleolar organizing regions (NOR) count and histopathological changes in rat's liver treated with methotrexate (MTX). Male Wistar rats aged 4 months, maintained in our institution were used in the present study. Animals were injected intraperitonealy with 3 different doses of methotrexate on alternative days for one week and the animals were sacrificed on the seventh day by using ether. Paraffin slides were prepared and histopathology as well as AgNOR studies were done. In the histopathological study, liver showed disruption of normal hepatic plates, vaculation of the hepatocytes, nuclear variability and enlarged blood vessels. In AgNOR study, data showed an overall decrease in the total count (numerical) from normal to 12 mg dose MTX. The total count includes small, medium and large sized AgNORs. A statistically high significant difference was found from normal to 12 mg dose MTX as well as between the doses. In conclusion, the genotoxicity in rats is well documented with high dose of methotrexate (12 mg/kg) compared to low dose (8 mg/kg). Methotrexate treatment reduces the transcription rate and ploidy. Methotrexate reduces the cell proliferation. Thus it reduces the count.",
author = "Deepthinath Raghunathan and Kurady, {Laxminarayana Bairy} and Ramachandra Bhat",
year = "2009",
month = "1",
day = "1",
language = "English",
volume = "8",
pages = "11--15",
journal = "Iranian Journal of Pharmacology and Therapeutics",
issn = "1735-2657",
publisher = "Academy of Medical Sciences of I.R. Iran",
number = "1",

}

Effect of methotrexate on AgNOR count in liver of wistar rats. / Raghunathan, Deepthinath; Kurady, Laxminarayana Bairy; Bhat, Ramachandra.

In: Iranian Journal of Pharmacology and Therapeutics, Vol. 8, No. 1, 01.01.2009, p. 11-15.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of methotrexate on AgNOR count in liver of wistar rats

AU - Raghunathan, Deepthinath

AU - Kurady, Laxminarayana Bairy

AU - Bhat, Ramachandra

PY - 2009/1/1

Y1 - 2009/1/1

N2 - A study was undertaken to evaluate the Nucleolar organizing regions (NOR) count and histopathological changes in rat's liver treated with methotrexate (MTX). Male Wistar rats aged 4 months, maintained in our institution were used in the present study. Animals were injected intraperitonealy with 3 different doses of methotrexate on alternative days for one week and the animals were sacrificed on the seventh day by using ether. Paraffin slides were prepared and histopathology as well as AgNOR studies were done. In the histopathological study, liver showed disruption of normal hepatic plates, vaculation of the hepatocytes, nuclear variability and enlarged blood vessels. In AgNOR study, data showed an overall decrease in the total count (numerical) from normal to 12 mg dose MTX. The total count includes small, medium and large sized AgNORs. A statistically high significant difference was found from normal to 12 mg dose MTX as well as between the doses. In conclusion, the genotoxicity in rats is well documented with high dose of methotrexate (12 mg/kg) compared to low dose (8 mg/kg). Methotrexate treatment reduces the transcription rate and ploidy. Methotrexate reduces the cell proliferation. Thus it reduces the count.

AB - A study was undertaken to evaluate the Nucleolar organizing regions (NOR) count and histopathological changes in rat's liver treated with methotrexate (MTX). Male Wistar rats aged 4 months, maintained in our institution were used in the present study. Animals were injected intraperitonealy with 3 different doses of methotrexate on alternative days for one week and the animals were sacrificed on the seventh day by using ether. Paraffin slides were prepared and histopathology as well as AgNOR studies were done. In the histopathological study, liver showed disruption of normal hepatic plates, vaculation of the hepatocytes, nuclear variability and enlarged blood vessels. In AgNOR study, data showed an overall decrease in the total count (numerical) from normal to 12 mg dose MTX. The total count includes small, medium and large sized AgNORs. A statistically high significant difference was found from normal to 12 mg dose MTX as well as between the doses. In conclusion, the genotoxicity in rats is well documented with high dose of methotrexate (12 mg/kg) compared to low dose (8 mg/kg). Methotrexate treatment reduces the transcription rate and ploidy. Methotrexate reduces the cell proliferation. Thus it reduces the count.

UR - http://www.scopus.com/inward/record.url?scp=77949449412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77949449412&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:77949449412

VL - 8

SP - 11

EP - 15

JO - Iranian Journal of Pharmacology and Therapeutics

JF - Iranian Journal of Pharmacology and Therapeutics

SN - 1735-2657

IS - 1

ER -