Effect of nucleants in photothermally assisted crystallization

T. Shilpa, Sajan D. George, Aseefhali Bankapur, Santhosh Chidangil, Aditya K. Dharmadhikari, Deepak Mathur, S. Madan Kumar, K. Byrappa, Abdul Ajees Abdul Salam

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Laser-induced crystallization is emerging as a promising technique to crystallize biomolecules like amino acids and proteins. The use of external materials as nucleants and novel seeding methods open new paths for protein crystallization. We report here the results of experiments that explore the effect of nucleants on laser-based crystallization of microlitre droplets of small molecules, amino acids, and proteins. The role of parameters like solute concentration, droplet volume, type and size of the nucleant, and laser power, are systematically investigated. In addition to crystallization of standard molecules like NaCl, KCl, and glycine, we demonstrate the crystallization of negatively (l-histidine), and positively (l-aspartic acid) charged amino acids and lysozyme protein. Single crystal X-ray diffraction and Raman spectroscopy studies unequivocally indicate that the nucleants do not alter the molecular structure of glycine, hydrogen bonding patterns, and packing. Localized vaporization of the solvent near the nucleant due to photothermal heating has enabled us to achieve rapid crystallization-within 3 s-at laser intensities of 0.1 MW cm-2, significantly lower than those reported earlier, with both saturated and unsaturated solutions. The outcome of the current experiments may be of utility in tackling various crystallization problems during the formation of crystals large enough to perform X-ray crystallography.

Original languageEnglish
Pages (from-to)870-882
Number of pages13
JournalPhotochemical and Photobiological Sciences
Volume16
Issue number6
DOIs
Publication statusPublished - 01-01-2017

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Crystallization
crystallization
proteins
amino acids
Lasers
glycine
Amino Acids
Glycine
lasers
Proteins
aspartic acid
Molecules
histidine
X ray crystallography
lysozyme
Biomolecules
inoculation
Muramidase
Vaporization
Histidine

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry

Cite this

Shilpa, T. ; George, Sajan D. ; Bankapur, Aseefhali ; Chidangil, Santhosh ; Dharmadhikari, Aditya K. ; Mathur, Deepak ; Madan Kumar, S. ; Byrappa, K. ; Abdul Salam, Abdul Ajees. / Effect of nucleants in photothermally assisted crystallization. In: Photochemical and Photobiological Sciences. 2017 ; Vol. 16, No. 6. pp. 870-882.
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Effect of nucleants in photothermally assisted crystallization. / Shilpa, T.; George, Sajan D.; Bankapur, Aseefhali; Chidangil, Santhosh; Dharmadhikari, Aditya K.; Mathur, Deepak; Madan Kumar, S.; Byrappa, K.; Abdul Salam, Abdul Ajees.

In: Photochemical and Photobiological Sciences, Vol. 16, No. 6, 01.01.2017, p. 870-882.

Research output: Contribution to journalArticle

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