Effect of Orexin-A infusion in to the Nucleus Accumbens on consummatory behaviour and alcohol preference in male Wistar rats

Santosh Mayannavar, K. S. Rashmi, Yalla Durga Rao, Saraswati Yadav, B. Ganaraja

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Effect of administration of Orexin-A into nucleus accumbens (NAcc) in relation to the regulation of feeding behavior and alcohol consumption at specific time intervals is relatively unknown.

Materials and methods: In this study, Male Wistar albino rats (n=54) weighing about 250±10 grams were implanted bilaterally with guide cannula (22 gauze) to target NAcc by stereotaxic surgery. Saline (0.9%) for control and Orexin-A for experimental groups (100 pmol or 250 pmol) were infused by Harvard picoplus pump. Food, water and alcohol (10%) consumption were measured at 1, 2, 4 and 24 hours to evaluate the effect of Orexin-A in fasted rats (24 hours). Preference study was carried out by two bottle choice test.

Results: Orexin-A infusion into NAcc showed significant increase in food at 1 hr in all groups compared to controls (p<0.05) and alcohol (p<0.02) intake. The changes were dose dependent. There was no noticeable preference or alcohol.

Conclusions for: These findings showed that Orexin-A in NAcc could be involved in feeding and drinking but not alcohol preference. The results highlight the effect of Orexin A infusion into NAcc in consummatory behaviour besides other hypothalamic and mesolimbic centres.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalIndian Journal of Physiology and Pharmacology
Volume58
Issue number4
Publication statusPublished - 01-10-2014

Fingerprint

Consummatory Behavior
Nucleus Accumbens
Wistar Rats
Alcohols
Alcohol Drinking
Food
Feeding Behavior
Orexins
Water

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology
  • Physiology (medical)

Cite this

@article{b8939cb1aa55484084f7d2756931fdcb,
title = "Effect of Orexin-A infusion in to the Nucleus Accumbens on consummatory behaviour and alcohol preference in male Wistar rats",
abstract = "Background: Effect of administration of Orexin-A into nucleus accumbens (NAcc) in relation to the regulation of feeding behavior and alcohol consumption at specific time intervals is relatively unknown.Materials and methods: In this study, Male Wistar albino rats (n=54) weighing about 250±10 grams were implanted bilaterally with guide cannula (22 gauze) to target NAcc by stereotaxic surgery. Saline (0.9{\%}) for control and Orexin-A for experimental groups (100 pmol or 250 pmol) were infused by Harvard picoplus pump. Food, water and alcohol (10{\%}) consumption were measured at 1, 2, 4 and 24 hours to evaluate the effect of Orexin-A in fasted rats (24 hours). Preference study was carried out by two bottle choice test.Results: Orexin-A infusion into NAcc showed significant increase in food at 1 hr in all groups compared to controls (p<0.05) and alcohol (p<0.02) intake. The changes were dose dependent. There was no noticeable preference or alcohol.Conclusions for: These findings showed that Orexin-A in NAcc could be involved in feeding and drinking but not alcohol preference. The results highlight the effect of Orexin A infusion into NAcc in consummatory behaviour besides other hypothalamic and mesolimbic centres.",
author = "Santosh Mayannavar and Rashmi, {K. S.} and Rao, {Yalla Durga} and Saraswati Yadav and B. Ganaraja",
year = "2014",
month = "10",
day = "1",
language = "English",
volume = "58",
pages = "319--326",
journal = "Indian Journal of Physiology and Pharmacology",
issn = "0019-5499",
publisher = "Association of Physiologists and Pharmacologists of India",
number = "4",

}

Effect of Orexin-A infusion in to the Nucleus Accumbens on consummatory behaviour and alcohol preference in male Wistar rats. / Mayannavar, Santosh; Rashmi, K. S.; Rao, Yalla Durga; Yadav, Saraswati; Ganaraja, B.

In: Indian Journal of Physiology and Pharmacology, Vol. 58, No. 4, 01.10.2014, p. 319-326.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of Orexin-A infusion in to the Nucleus Accumbens on consummatory behaviour and alcohol preference in male Wistar rats

AU - Mayannavar, Santosh

AU - Rashmi, K. S.

AU - Rao, Yalla Durga

AU - Yadav, Saraswati

AU - Ganaraja, B.

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Background: Effect of administration of Orexin-A into nucleus accumbens (NAcc) in relation to the regulation of feeding behavior and alcohol consumption at specific time intervals is relatively unknown.Materials and methods: In this study, Male Wistar albino rats (n=54) weighing about 250±10 grams were implanted bilaterally with guide cannula (22 gauze) to target NAcc by stereotaxic surgery. Saline (0.9%) for control and Orexin-A for experimental groups (100 pmol or 250 pmol) were infused by Harvard picoplus pump. Food, water and alcohol (10%) consumption were measured at 1, 2, 4 and 24 hours to evaluate the effect of Orexin-A in fasted rats (24 hours). Preference study was carried out by two bottle choice test.Results: Orexin-A infusion into NAcc showed significant increase in food at 1 hr in all groups compared to controls (p<0.05) and alcohol (p<0.02) intake. The changes were dose dependent. There was no noticeable preference or alcohol.Conclusions for: These findings showed that Orexin-A in NAcc could be involved in feeding and drinking but not alcohol preference. The results highlight the effect of Orexin A infusion into NAcc in consummatory behaviour besides other hypothalamic and mesolimbic centres.

AB - Background: Effect of administration of Orexin-A into nucleus accumbens (NAcc) in relation to the regulation of feeding behavior and alcohol consumption at specific time intervals is relatively unknown.Materials and methods: In this study, Male Wistar albino rats (n=54) weighing about 250±10 grams were implanted bilaterally with guide cannula (22 gauze) to target NAcc by stereotaxic surgery. Saline (0.9%) for control and Orexin-A for experimental groups (100 pmol or 250 pmol) were infused by Harvard picoplus pump. Food, water and alcohol (10%) consumption were measured at 1, 2, 4 and 24 hours to evaluate the effect of Orexin-A in fasted rats (24 hours). Preference study was carried out by two bottle choice test.Results: Orexin-A infusion into NAcc showed significant increase in food at 1 hr in all groups compared to controls (p<0.05) and alcohol (p<0.02) intake. The changes were dose dependent. There was no noticeable preference or alcohol.Conclusions for: These findings showed that Orexin-A in NAcc could be involved in feeding and drinking but not alcohol preference. The results highlight the effect of Orexin A infusion into NAcc in consummatory behaviour besides other hypothalamic and mesolimbic centres.

UR - http://www.scopus.com/inward/record.url?scp=84915817678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84915817678&partnerID=8YFLogxK

M3 - Article

VL - 58

SP - 319

EP - 326

JO - Indian Journal of Physiology and Pharmacology

JF - Indian Journal of Physiology and Pharmacology

SN - 0019-5499

IS - 4

ER -