Effect of pH on the adherence, surface hydrophobicity and the biofilm formation of Gardnerella vaginalis

Udayalaxmi, Gopalkrishna Bhat, Subbannayya Kotigadde, Shashidhar Kotian

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Bacterial vaginosis is a common cause of the abnormal vaginal discharge in women of the reproductive age group. Although bacterial vaginosis is prevalent, not much progress has been made in identifying the factors which are responsible for and those which are associated with bacterial vaginosis and its pathophysiology. Here, we would like to evaluate the effect of the change in the pH of the vagina on the virulence factors of Gardnerella vaginalis, the main organism which has been indicated in this vaginal condition. Objective: The objective of the present study was to observe the effect of pH on the adherence, surface hydrophobicity and the biofilm formation of 10 strains of Gardnerella vaginalis which were isolated from cases of bacterial vaginosis. Result: We found that the adherence to the vaginal epithelial cells and the surface hydrophobicity was maximum at a lower pH (pH- 3,4,5) and minimum at a higher pH (pH- 6,7), but the pH did not have a significant effect on the biofilm formation. Conclusion: An increase in the pH of the vagina which is observed during bacterial vaginosis, probably occurs much later during the disease. During the early part of the disease, the bacteria adhere to the vaginal epithelium, multiply in large numbers and form a thick biofilm which is not affected by the rise in pH, which occurs probably due to the metabolic activities of this large bacterial population. This also explains the fact that all women with bacterial vaginosis do not have an elevated vaginal pH.

Original languageEnglish
Pages (from-to)967-969
Number of pages3
JournalJournal of Clinical and Diagnostic Research
Volume6
Issue number6
Publication statusPublished - 10-08-2012

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Gardnerella vaginalis
Biofilms
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Bacterial Vaginosis
Virulence Factors
Bacteria
Vagina
Vaginal Discharge
Epithelium
Age Groups
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Clinical Biochemistry

Cite this

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abstract = "Background: Bacterial vaginosis is a common cause of the abnormal vaginal discharge in women of the reproductive age group. Although bacterial vaginosis is prevalent, not much progress has been made in identifying the factors which are responsible for and those which are associated with bacterial vaginosis and its pathophysiology. Here, we would like to evaluate the effect of the change in the pH of the vagina on the virulence factors of Gardnerella vaginalis, the main organism which has been indicated in this vaginal condition. Objective: The objective of the present study was to observe the effect of pH on the adherence, surface hydrophobicity and the biofilm formation of 10 strains of Gardnerella vaginalis which were isolated from cases of bacterial vaginosis. Result: We found that the adherence to the vaginal epithelial cells and the surface hydrophobicity was maximum at a lower pH (pH- 3,4,5) and minimum at a higher pH (pH- 6,7), but the pH did not have a significant effect on the biofilm formation. Conclusion: An increase in the pH of the vagina which is observed during bacterial vaginosis, probably occurs much later during the disease. During the early part of the disease, the bacteria adhere to the vaginal epithelium, multiply in large numbers and form a thick biofilm which is not affected by the rise in pH, which occurs probably due to the metabolic activities of this large bacterial population. This also explains the fact that all women with bacterial vaginosis do not have an elevated vaginal pH.",
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Effect of pH on the adherence, surface hydrophobicity and the biofilm formation of Gardnerella vaginalis. / Udayalaxmi, ; Bhat, Gopalkrishna; Kotigadde, Subbannayya; Kotian, Shashidhar.

In: Journal of Clinical and Diagnostic Research, Vol. 6, No. 6, 10.08.2012, p. 967-969.

Research output: Contribution to journalArticle

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AU - Kotian, Shashidhar

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