Effect of phenotypic switching on expression of virulence factors by Candida albicans causing candidiasis in diabetic patients

Georgy Antony, Vishwas Saralaya, K. Gopalkrishna Bhat, M. Shalini Shenoy, P. G. Shivananda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A total of 110 strains belonging to seven species of Candida were isolated from various forms of candidiasis in diabetic patients. They were Candida albicans 53 (47%), Candida tropicalis 36 (33%), Candida glabrata 9 (8%), Candida parapsilosis 4 (4%), Candida guilliermondii 2 (2%), Candida krusei 5 (5%) and Candida kefyr 1 (1%). All 53 strains of C. albicans isolated were observed to express virulence factors such as cell surface hydrophobicity (CSH), adherence to human buccal epithelial cell (BEC) and proteinase activity (100%), while phospholipase activity was observed in 52 (98%). Phenotypic switching and its influence on the pathogenicity of C. albicans were studied. Two C. albicans strains isolated from oral and vaginal thrush, respectively, in diabetic individuals, and the control strain C. albicans NCPF 3153A were induced to undergo phenotypic switching by exposure to UV light and the degree of expression of virulence factors by the different morphological forms was determined. Three different morphological forms of C. albicans were obtained, namely Star (S), Wrinkled (W) and Ring (R) types from the original Smooth (O) variety. It was found that proteinase activity was greatest with the W type followed by the R type then the O type. The S type produced the least proteinase. The phospholipase activity was greatest with O type followed by R type. The W and S types produced the least phospholipase. Expression of CSH and adherence was greatest in the O type followed by the R and then the W type and finally the S type. Differential expression of virulence factors occurs with different phenotypic forms of C. albicans and this may provide a particular morphological type with a distinct advantage over other types in causing candidiasis.

Original languageEnglish
Pages (from-to)202-205
Number of pages4
JournalRevista Iberoamericana de Micologia
Volume26
Issue number3
DOIs
Publication statusPublished - 30-09-2009

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Candidiasis
Virulence Factors
Candida albicans
Candida
Phospholipases
Peptide Hydrolases
Hydrophobic and Hydrophilic Interactions
Candida tropicalis
Candida glabrata
Oral Candidiasis
Cheek
Ultraviolet Rays
Virulence
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Cite this

Antony, Georgy ; Saralaya, Vishwas ; Gopalkrishna Bhat, K. ; Shalini Shenoy, M. ; Shivananda, P. G. / Effect of phenotypic switching on expression of virulence factors by Candida albicans causing candidiasis in diabetic patients. In: Revista Iberoamericana de Micologia. 2009 ; Vol. 26, No. 3. pp. 202-205.
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abstract = "A total of 110 strains belonging to seven species of Candida were isolated from various forms of candidiasis in diabetic patients. They were Candida albicans 53 (47{\%}), Candida tropicalis 36 (33{\%}), Candida glabrata 9 (8{\%}), Candida parapsilosis 4 (4{\%}), Candida guilliermondii 2 (2{\%}), Candida krusei 5 (5{\%}) and Candida kefyr 1 (1{\%}). All 53 strains of C. albicans isolated were observed to express virulence factors such as cell surface hydrophobicity (CSH), adherence to human buccal epithelial cell (BEC) and proteinase activity (100{\%}), while phospholipase activity was observed in 52 (98{\%}). Phenotypic switching and its influence on the pathogenicity of C. albicans were studied. Two C. albicans strains isolated from oral and vaginal thrush, respectively, in diabetic individuals, and the control strain C. albicans NCPF 3153A were induced to undergo phenotypic switching by exposure to UV light and the degree of expression of virulence factors by the different morphological forms was determined. Three different morphological forms of C. albicans were obtained, namely Star (S), Wrinkled (W) and Ring (R) types from the original Smooth (O) variety. It was found that proteinase activity was greatest with the W type followed by the R type then the O type. The S type produced the least proteinase. The phospholipase activity was greatest with O type followed by R type. The W and S types produced the least phospholipase. Expression of CSH and adherence was greatest in the O type followed by the R and then the W type and finally the S type. Differential expression of virulence factors occurs with different phenotypic forms of C. albicans and this may provide a particular morphological type with a distinct advantage over other types in causing candidiasis.",
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Effect of phenotypic switching on expression of virulence factors by Candida albicans causing candidiasis in diabetic patients. / Antony, Georgy; Saralaya, Vishwas; Gopalkrishna Bhat, K.; Shalini Shenoy, M.; Shivananda, P. G.

In: Revista Iberoamericana de Micologia, Vol. 26, No. 3, 30.09.2009, p. 202-205.

Research output: Contribution to journalArticle

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