Effect of prenatal isotretinoin exposure on neuronal population of prefrontal cortex in rats

Divya Premchandran, Sampath Madhyastha, Vasudha Saralaya, Jai Aditya, Teresa Joy, Sudhanshu Sahu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The isotretinoin, a 13-cis-retinoic acid is used in the most severe acne. In humans, isotretinoin treatment is known to cause psychiatric side effects like depression. It is also known to be teratogenic resulting in reduced IQ scores in children who have exposed to isotretinoin during prenatal development. In animal model studies it is known to cause craniofacial deformities including cleft palate. Isotretinoin is known to affect the adult neurogenesis, but there are no studies indicating the teratogenic effect of isotretinoin on intra-uterine neurogenesis. Hence in the present study we investigate teratogenic effect on neuronal population of prefrontal cortex.Pregnant Wistar rats were exposed to either 8 or 16mg/kg dose of body weight of isotretinoin during early or mid-gestationalperiod of pregnancy. Pups were sacrificed at postnatal day 7 or 21; brains were removed and processed for histological studies using cresyl violet staining. Neuronal population of the prefrontal cortex was quantified. Isotretinoin treatment resulted in 10% mortality at birth in day 6 to 10 treatment schedule. The results of neuronal assay clearly demonstrate that teratogenic effect of isotretinoin is more when administered during early gestational period in rats& the neurotoxic effects were not dependent on the dose of isotretinoin.

Original languageEnglish
Pages (from-to)902-911
Number of pages10
JournalResearch Journal of Pharmaceutical, Biological and Chemical Sciences
Volume4
Issue number1
Publication statusPublished - 01-2013
Externally publishedYes

Fingerprint

Isotretinoin
Prefrontal Cortex
Rats
Population
Neurogenesis
Acne Vulgaris
Cleft Palate
Psychiatry
Wistar Rats
Assays
Brain
Appointments and Schedules
Animals
Therapeutics
Animal Models
Body Weight
Parturition
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{b6e559eab26547a797ae7a25f14c168f,
title = "Effect of prenatal isotretinoin exposure on neuronal population of prefrontal cortex in rats",
abstract = "The isotretinoin, a 13-cis-retinoic acid is used in the most severe acne. In humans, isotretinoin treatment is known to cause psychiatric side effects like depression. It is also known to be teratogenic resulting in reduced IQ scores in children who have exposed to isotretinoin during prenatal development. In animal model studies it is known to cause craniofacial deformities including cleft palate. Isotretinoin is known to affect the adult neurogenesis, but there are no studies indicating the teratogenic effect of isotretinoin on intra-uterine neurogenesis. Hence in the present study we investigate teratogenic effect on neuronal population of prefrontal cortex.Pregnant Wistar rats were exposed to either 8 or 16mg/kg dose of body weight of isotretinoin during early or mid-gestationalperiod of pregnancy. Pups were sacrificed at postnatal day 7 or 21; brains were removed and processed for histological studies using cresyl violet staining. Neuronal population of the prefrontal cortex was quantified. Isotretinoin treatment resulted in 10{\%} mortality at birth in day 6 to 10 treatment schedule. The results of neuronal assay clearly demonstrate that teratogenic effect of isotretinoin is more when administered during early gestational period in rats& the neurotoxic effects were not dependent on the dose of isotretinoin.",
author = "Divya Premchandran and Sampath Madhyastha and Vasudha Saralaya and Jai Aditya and Teresa Joy and Sudhanshu Sahu",
year = "2013",
month = "1",
language = "English",
volume = "4",
pages = "902--911",
journal = "Research Journal of Pharmaceutical, Biological and Chemical Sciences",
issn = "0975-8585",
publisher = "RJPBCS",
number = "1",

}

Effect of prenatal isotretinoin exposure on neuronal population of prefrontal cortex in rats. / Premchandran, Divya; Madhyastha, Sampath; Saralaya, Vasudha; Aditya, Jai; Joy, Teresa; Sahu, Sudhanshu.

In: Research Journal of Pharmaceutical, Biological and Chemical Sciences, Vol. 4, No. 1, 01.2013, p. 902-911.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of prenatal isotretinoin exposure on neuronal population of prefrontal cortex in rats

AU - Premchandran, Divya

AU - Madhyastha, Sampath

AU - Saralaya, Vasudha

AU - Aditya, Jai

AU - Joy, Teresa

AU - Sahu, Sudhanshu

PY - 2013/1

Y1 - 2013/1

N2 - The isotretinoin, a 13-cis-retinoic acid is used in the most severe acne. In humans, isotretinoin treatment is known to cause psychiatric side effects like depression. It is also known to be teratogenic resulting in reduced IQ scores in children who have exposed to isotretinoin during prenatal development. In animal model studies it is known to cause craniofacial deformities including cleft palate. Isotretinoin is known to affect the adult neurogenesis, but there are no studies indicating the teratogenic effect of isotretinoin on intra-uterine neurogenesis. Hence in the present study we investigate teratogenic effect on neuronal population of prefrontal cortex.Pregnant Wistar rats were exposed to either 8 or 16mg/kg dose of body weight of isotretinoin during early or mid-gestationalperiod of pregnancy. Pups were sacrificed at postnatal day 7 or 21; brains were removed and processed for histological studies using cresyl violet staining. Neuronal population of the prefrontal cortex was quantified. Isotretinoin treatment resulted in 10% mortality at birth in day 6 to 10 treatment schedule. The results of neuronal assay clearly demonstrate that teratogenic effect of isotretinoin is more when administered during early gestational period in rats& the neurotoxic effects were not dependent on the dose of isotretinoin.

AB - The isotretinoin, a 13-cis-retinoic acid is used in the most severe acne. In humans, isotretinoin treatment is known to cause psychiatric side effects like depression. It is also known to be teratogenic resulting in reduced IQ scores in children who have exposed to isotretinoin during prenatal development. In animal model studies it is known to cause craniofacial deformities including cleft palate. Isotretinoin is known to affect the adult neurogenesis, but there are no studies indicating the teratogenic effect of isotretinoin on intra-uterine neurogenesis. Hence in the present study we investigate teratogenic effect on neuronal population of prefrontal cortex.Pregnant Wistar rats were exposed to either 8 or 16mg/kg dose of body weight of isotretinoin during early or mid-gestationalperiod of pregnancy. Pups were sacrificed at postnatal day 7 or 21; brains were removed and processed for histological studies using cresyl violet staining. Neuronal population of the prefrontal cortex was quantified. Isotretinoin treatment resulted in 10% mortality at birth in day 6 to 10 treatment schedule. The results of neuronal assay clearly demonstrate that teratogenic effect of isotretinoin is more when administered during early gestational period in rats& the neurotoxic effects were not dependent on the dose of isotretinoin.

UR - http://www.scopus.com/inward/record.url?scp=84874691341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874691341&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84874691341

VL - 4

SP - 902

EP - 911

JO - Research Journal of Pharmaceutical, Biological and Chemical Sciences

JF - Research Journal of Pharmaceutical, Biological and Chemical Sciences

SN - 0975-8585

IS - 1

ER -