Effect of stress on production of heat labile enterotoxin by Escherichia coli

A. Hegde, G. K. Bhat, S. Mallya

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Enterotoxigenic Escherichia coli (ETEC) is an important pathogen responsible for secretory diarrhoea. The production of heat labile enterotoxin (LT), by ETEC, is largely responsible for the pathogenesis of diarrhoea. In the present study we investigated the effect of stress factors such as temperature, pH, osmotic stress and nutritional limitation on the production of LT by ETEC using in-house GMI-ELISA. Four strains of E. coli consisting, one standard strain MTCC 723 and three clinical isolates were used in the study. Maximum amount of LT (OD 3.285) was produced at 370 C followed by 40 0 C (OD 3.305). Growth of E. coli in medium with pH 8.6 resulted in maximum amount of LT production (OD 3.489). LT was not detectable when bacteria were grown in medium with pH ≤7.2 and ≥9.2. Sodium chloride concentration of 0.2 M stimulated maximum amount of LT production. Maximum amount of LT was produced when the bacteria were grown in medium containing 2.5g/l of glucose. All the stress factors had a significant effect on the LT production by E. coli , though quantitative differences in the various strains were observed.

Original languageEnglish
Pages (from-to)325-328
Number of pages4
JournalIndian Journal of Medical Microbiology
Volume27
Issue number4
DOIs
Publication statusPublished - 01-10-2009

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Enterotoxigenic Escherichia coli
Thermogenesis
Enterotoxins
Escherichia coli
Diarrhea
Bacteria
Osmotic Pressure
Sodium Chloride
Enzyme-Linked Immunosorbent Assay
Glucose
Temperature
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

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Effect of stress on production of heat labile enterotoxin by Escherichia coli. / Hegde, A.; Bhat, G. K.; Mallya, S.

In: Indian Journal of Medical Microbiology, Vol. 27, No. 4, 01.10.2009, p. 325-328.

Research output: Contribution to journalArticle

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