Effect of various concentrations of acyclovir on cell survival and micronuclei induction on cultured HeLa cells

Ganesh Chandra Jagetia, R. Aruna

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The HeLa cells were treated with 0, 0.01, 0.1, 1, 10 and 100 μM acyclovir (ACV) for 8 h duration and the growth kinetics, cell survival and micronuclei induction were determined. Treatment of HeLa cells with various concentrations of ACV resulted in a concentration-dependent decline in growth kinetics, cell proliferation indices and cell survival. ACV, 100 μM, completely inhibited cell division, where no appreciable changes in cell number were observed from 1 to 5 days post-treatment. This is reflected in cell survival, where the surviving fraction of cells was reduced to 1/2 at 100 μM ACV. Conversely, the frequency of micronuclei showed a concentration-dependent elevation at 20, 30 and 40 h post-treatment. ACV not only induced one micronuclei-bearing binucleate cell but also binucleate cells bearing two and multiple micronuclei in a concentration-dependent manner. The micronuclei frequency increased with time up to 30 h post-treatment and declined thereafter. The relationship between micronuclei induction and cell survival was determined by plotting the former on Y- and the latter on X-axes, respectively. The surviving fraction of cells declined with the elevation in micronuclei frequency and a best fit was observed for linear quadratic formalism. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)155-165
Number of pages11
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume446
Issue number2
DOIs
Publication statusPublished - 13-12-1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Health, Toxicology and Mutagenesis
  • Genetics

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