Effect of various concentrations of acyclovir on cell survival and micronuclei induction on cultured HeLa cells

Ganesh Chandra Jagetia, R. Aruna

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The HeLa cells were treated with 0, 0.01, 0.1, 1, 10 and 100 μM acyclovir (ACV) for 8 h duration and the growth kinetics, cell survival and micronuclei induction were determined. Treatment of HeLa cells with various concentrations of ACV resulted in a concentration-dependent decline in growth kinetics, cell proliferation indices and cell survival. ACV, 100 μM, completely inhibited cell division, where no appreciable changes in cell number were observed from 1 to 5 days post-treatment. This is reflected in cell survival, where the surviving fraction of cells was reduced to 1/2 at 100 μM ACV. Conversely, the frequency of micronuclei showed a concentration-dependent elevation at 20, 30 and 40 h post-treatment. ACV not only induced one micronuclei-bearing binucleate cell but also binucleate cells bearing two and multiple micronuclei in a concentration-dependent manner. The micronuclei frequency increased with time up to 30 h post-treatment and declined thereafter. The relationship between micronuclei induction and cell survival was determined by plotting the former on Y- and the latter on X-axes, respectively. The surviving fraction of cells declined with the elevation in micronuclei frequency and a best fit was observed for linear quadratic formalism. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)155-165
Number of pages11
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume446
Issue number2
DOIs
Publication statusPublished - 13-12-1999
Externally publishedYes

Fingerprint

Acyclovir
HeLa Cells
Cultured Cells
Cell Survival
Growth
Cell Division
Cell Count
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Health, Toxicology and Mutagenesis
  • Genetics

Cite this

@article{996c8c043a254f9c8c2b921dbadf4eae,
title = "Effect of various concentrations of acyclovir on cell survival and micronuclei induction on cultured HeLa cells",
abstract = "The HeLa cells were treated with 0, 0.01, 0.1, 1, 10 and 100 μM acyclovir (ACV) for 8 h duration and the growth kinetics, cell survival and micronuclei induction were determined. Treatment of HeLa cells with various concentrations of ACV resulted in a concentration-dependent decline in growth kinetics, cell proliferation indices and cell survival. ACV, 100 μM, completely inhibited cell division, where no appreciable changes in cell number were observed from 1 to 5 days post-treatment. This is reflected in cell survival, where the surviving fraction of cells was reduced to 1/2 at 100 μM ACV. Conversely, the frequency of micronuclei showed a concentration-dependent elevation at 20, 30 and 40 h post-treatment. ACV not only induced one micronuclei-bearing binucleate cell but also binucleate cells bearing two and multiple micronuclei in a concentration-dependent manner. The micronuclei frequency increased with time up to 30 h post-treatment and declined thereafter. The relationship between micronuclei induction and cell survival was determined by plotting the former on Y- and the latter on X-axes, respectively. The surviving fraction of cells declined with the elevation in micronuclei frequency and a best fit was observed for linear quadratic formalism. Copyright (C) 1999 Elsevier Science B.V.",
author = "Jagetia, {Ganesh Chandra} and R. Aruna",
year = "1999",
month = "12",
day = "13",
doi = "10.1016/S1383-5718(99)00159-X",
language = "English",
volume = "446",
pages = "155--165",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
issn = "1383-5718",
publisher = "Elsevier",
number = "2",

}

Effect of various concentrations of acyclovir on cell survival and micronuclei induction on cultured HeLa cells. / Jagetia, Ganesh Chandra; Aruna, R.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 446, No. 2, 13.12.1999, p. 155-165.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of various concentrations of acyclovir on cell survival and micronuclei induction on cultured HeLa cells

AU - Jagetia, Ganesh Chandra

AU - Aruna, R.

PY - 1999/12/13

Y1 - 1999/12/13

N2 - The HeLa cells were treated with 0, 0.01, 0.1, 1, 10 and 100 μM acyclovir (ACV) for 8 h duration and the growth kinetics, cell survival and micronuclei induction were determined. Treatment of HeLa cells with various concentrations of ACV resulted in a concentration-dependent decline in growth kinetics, cell proliferation indices and cell survival. ACV, 100 μM, completely inhibited cell division, where no appreciable changes in cell number were observed from 1 to 5 days post-treatment. This is reflected in cell survival, where the surviving fraction of cells was reduced to 1/2 at 100 μM ACV. Conversely, the frequency of micronuclei showed a concentration-dependent elevation at 20, 30 and 40 h post-treatment. ACV not only induced one micronuclei-bearing binucleate cell but also binucleate cells bearing two and multiple micronuclei in a concentration-dependent manner. The micronuclei frequency increased with time up to 30 h post-treatment and declined thereafter. The relationship between micronuclei induction and cell survival was determined by plotting the former on Y- and the latter on X-axes, respectively. The surviving fraction of cells declined with the elevation in micronuclei frequency and a best fit was observed for linear quadratic formalism. Copyright (C) 1999 Elsevier Science B.V.

AB - The HeLa cells were treated with 0, 0.01, 0.1, 1, 10 and 100 μM acyclovir (ACV) for 8 h duration and the growth kinetics, cell survival and micronuclei induction were determined. Treatment of HeLa cells with various concentrations of ACV resulted in a concentration-dependent decline in growth kinetics, cell proliferation indices and cell survival. ACV, 100 μM, completely inhibited cell division, where no appreciable changes in cell number were observed from 1 to 5 days post-treatment. This is reflected in cell survival, where the surviving fraction of cells was reduced to 1/2 at 100 μM ACV. Conversely, the frequency of micronuclei showed a concentration-dependent elevation at 20, 30 and 40 h post-treatment. ACV not only induced one micronuclei-bearing binucleate cell but also binucleate cells bearing two and multiple micronuclei in a concentration-dependent manner. The micronuclei frequency increased with time up to 30 h post-treatment and declined thereafter. The relationship between micronuclei induction and cell survival was determined by plotting the former on Y- and the latter on X-axes, respectively. The surviving fraction of cells declined with the elevation in micronuclei frequency and a best fit was observed for linear quadratic formalism. Copyright (C) 1999 Elsevier Science B.V.

UR - http://www.scopus.com/inward/record.url?scp=0032776240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032776240&partnerID=8YFLogxK

U2 - 10.1016/S1383-5718(99)00159-X

DO - 10.1016/S1383-5718(99)00159-X

M3 - Article

VL - 446

SP - 155

EP - 165

JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

SN - 1383-5718

IS - 2

ER -