Effect of vindesine sulfate on the radiation-induced alterations in mouse spermatogenesis

A flow cytometric evaluation

Ganesh Chandra Jagetia, P. Jyothi, H. Krishnamurthy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effect of 0.05 mg/kg body weight of vindesine sulfate was studied on the radiation-induced changes in mouse spermatogenesis at 1, 2, 7, 14, 21, 28, 35 and 70 days post-irradiation. Vindesine administration before exposure to 0, 0.5, 1, 2, and 3 Gy γ-irradiation resulted in an increase in the radiation-induced perturbations of mouse spermatogenesis at various post-exposure time periods studied. A significant reduction in testicular weight was observed in both DDW + irradiated and VDS + irradiated groups at various post-irradiation time periods, depending on the exposure dose. Vindesine pretreatment resulted in an enhanced killing of spermatogonial cells at day 2 post-exposure at all the exposure doses, except 3 Gy when compared to DDW + irradiated controls. Consequently, the tetraploid (4C) population declined significantly by day 14 post-irradiation followed by a severe depletion in round spermatids (1C) by day 21 post-irradiation. The dose-response relationship for 4C and 1C populations was linear-quadratic at days 14 and 21, respectively. A significant elevation was observed in HC population from days I to 21 depending on the exposure dose. The germ cell ratios, viz. 4C:2C, 4C:S-phase, 1C:2C and 1C:4C, showed a significant decline in the VDS + irradiated group when compared to the DDW + irradiated group at various time periods, depending on the exposure dose.

Original languageEnglish
Pages (from-to)163-174
Number of pages12
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume398
Issue number1-2
DOIs
Publication statusPublished - 28-02-1998
Externally publishedYes

Fingerprint

Vindesine
Spermatogenesis
Radiation
Population
Tetraploidy
Spermatids
S Phase
Germ Cells
Body Weight
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Effect of vindesine sulfate on the radiation-induced alterations in mouse spermatogenesis: A flow cytometric evaluation",
abstract = "The effect of 0.05 mg/kg body weight of vindesine sulfate was studied on the radiation-induced changes in mouse spermatogenesis at 1, 2, 7, 14, 21, 28, 35 and 70 days post-irradiation. Vindesine administration before exposure to 0, 0.5, 1, 2, and 3 Gy γ-irradiation resulted in an increase in the radiation-induced perturbations of mouse spermatogenesis at various post-exposure time periods studied. A significant reduction in testicular weight was observed in both DDW + irradiated and VDS + irradiated groups at various post-irradiation time periods, depending on the exposure dose. Vindesine pretreatment resulted in an enhanced killing of spermatogonial cells at day 2 post-exposure at all the exposure doses, except 3 Gy when compared to DDW + irradiated controls. Consequently, the tetraploid (4C) population declined significantly by day 14 post-irradiation followed by a severe depletion in round spermatids (1C) by day 21 post-irradiation. The dose-response relationship for 4C and 1C populations was linear-quadratic at days 14 and 21, respectively. A significant elevation was observed in HC population from days I to 21 depending on the exposure dose. The germ cell ratios, viz. 4C:2C, 4C:S-phase, 1C:2C and 1C:4C, showed a significant decline in the VDS + irradiated group when compared to the DDW + irradiated group at various time periods, depending on the exposure dose.",
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Effect of vindesine sulfate on the radiation-induced alterations in mouse spermatogenesis : A flow cytometric evaluation. / Jagetia, Ganesh Chandra; Jyothi, P.; Krishnamurthy, H.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 398, No. 1-2, 28.02.1998, p. 163-174.

Research output: Contribution to journalArticle

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T1 - Effect of vindesine sulfate on the radiation-induced alterations in mouse spermatogenesis

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