Effects of adriamycin and daunomycin on ornithine decarboxylase activity and DNA synthesis in mouse epidermal cells

K. Satyamoorthy, E. M. Perchellet, J. P. Perchellet

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60%, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50% or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca2+-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.

    Original languageEnglish
    Pages (from-to)459-463
    Number of pages5
    JournalToxicology in Vitro
    Volume6
    Issue number5
    DOIs
    Publication statusPublished - 1992

    Fingerprint

    Daunorubicin
    Ornithine Decarboxylase
    Doxorubicin
    DNA
    Tetradecanoylphorbol Acetate
    Acetates
    Anthracyclines
    Verapamil
    Thermodynamic properties
    Anti-Bacterial Agents

    All Science Journal Classification (ASJC) codes

    • Toxicology

    Cite this

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    title = "Effects of adriamycin and daunomycin on ornithine decarboxylase activity and DNA synthesis in mouse epidermal cells",
    abstract = "Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60{\%}, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50{\%} or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca2+-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.",
    author = "K. Satyamoorthy and Perchellet, {E. M.} and Perchellet, {J. P.}",
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    Effects of adriamycin and daunomycin on ornithine decarboxylase activity and DNA synthesis in mouse epidermal cells. / Satyamoorthy, K.; Perchellet, E. M.; Perchellet, J. P.

    In: Toxicology in Vitro, Vol. 6, No. 5, 1992, p. 459-463.

    Research output: Contribution to journalArticle

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    AU - Satyamoorthy, K.

    AU - Perchellet, E. M.

    AU - Perchellet, J. P.

    PY - 1992

    Y1 - 1992

    N2 - Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60%, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50% or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca2+-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.

    AB - Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60%, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50% or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca2+-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.

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