TY - JOUR
T1 - Effects of adriamycin and daunomycin on ornithine decarboxylase activity and DNA synthesis in mouse epidermal cells
AU - Satyamoorthy, K.
AU - Perchellet, E. M.
AU - Perchellet, J. P.
PY - 1992
Y1 - 1992
N2 - Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60%, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50% or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca2+-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.
AB - Ornithine decarboxylase (ODC) activity is induced when epidermal cells are incubated for 5-12 hr in the presence of 10 μm-adriamycin (ADR). The magnitude and duration of ODC induction by 5 μm-daunomycin (DAU) are much smaller. At 10 μm, ADR does not alter DNA synthesis but DAU inhibits ODC activity and DNA synthesis by 40 and 60%, respectively. ADR (10 μm) and, to a lesser degree, DAU (5 μm) also enhance 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity, but, in contrast to ADR, 10-50 μm-DAU inhibit the ODC response to TPA by 50% or more. The induction and superinduction of ODC activities by ADR and/or TPA are all inhibited by the Ca2+-channel blocker verapamil. The ODC-inducing activity of ADR, therefore, may have a role in the mechanism by which mouse epidermal cells escape from the cytotoxic activity of this anthracycline antibiotic.
UR - http://www.scopus.com/inward/record.url?scp=0026713796&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026713796&partnerID=8YFLogxK
U2 - 10.1016/0887-2333(92)90053-T
DO - 10.1016/0887-2333(92)90053-T
M3 - Article
AN - SCOPUS:0026713796
SN - 0887-2333
VL - 6
SP - 459
EP - 463
JO - Toxicology in Vitro
JF - Toxicology in Vitro
IS - 5
ER -