Effects of evans blue and amiloride on anti-diuretic hormone (ADH)-induced sodium transport across frog (Rana hexadactyla) Skin

Silviya Rajakumari Jared, Jonakuty Prakasa Rao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The epithelial sodium channel (ENaC) has four subunits, namely α (alpha), β (beta), γ (gamma) and δ (delta). The functional ENaC is formed by the combination of either αβγ or δβγ subunits. The aim of the present study is to determine the combination of ENaC subunits predominant on the apical side of the frog skin, and the effect of ADH on sodium transport though these two ENaCs subunit combinations. The ventral abdominal skin of the frog, Rana hexadactyla was mounted in an Ussing-type chamber. The voltage-clamp method was performed to measure the ionic transport across the frog skin with normal Ringer solution (NR) on both sides. Evans blue (300 M) and amiloride (100 M) were added to the NR on the apical side and ADH (40 nM) was added on the serosal side. Statistical significance was analyzed by Student's paired t-test and repeated-measures ANOVA, P < 0.05 was considered significant. This study suggests that the ENaC of the frog skin consist of both αβγ and δβγ subunit combinations on the apical side. Though both types of subunit combination are present, the αβγ type was found to be more common than δβγ. ADH increases the sodium transport across the frog skin. The effect of ADH on sodium transport is achieved through the combination of δ-subunits, not through the combination of a-subunits in the skin of Pana hexadactyla.

Original languageEnglish
Pages (from-to)402-407
Number of pages6
JournalZoological Science
Volume30
Issue number5
DOIs
Publication statusPublished - 01-05-2013
Externally publishedYes

Fingerprint

vasopressin
skin (animal)
frogs
sodium
sodium channels
Euphlyctis hexadactylus
analysis of variance

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

@article{b08585758b3243f78116fe55fbb897e3,
title = "Effects of evans blue and amiloride on anti-diuretic hormone (ADH)-induced sodium transport across frog (Rana hexadactyla) Skin",
abstract = "The epithelial sodium channel (ENaC) has four subunits, namely α (alpha), β (beta), γ (gamma) and δ (delta). The functional ENaC is formed by the combination of either αβγ or δβγ subunits. The aim of the present study is to determine the combination of ENaC subunits predominant on the apical side of the frog skin, and the effect of ADH on sodium transport though these two ENaCs subunit combinations. The ventral abdominal skin of the frog, Rana hexadactyla was mounted in an Ussing-type chamber. The voltage-clamp method was performed to measure the ionic transport across the frog skin with normal Ringer solution (NR) on both sides. Evans blue (300 M) and amiloride (100 M) were added to the NR on the apical side and ADH (40 nM) was added on the serosal side. Statistical significance was analyzed by Student's paired t-test and repeated-measures ANOVA, P < 0.05 was considered significant. This study suggests that the ENaC of the frog skin consist of both αβγ and δβγ subunit combinations on the apical side. Though both types of subunit combination are present, the αβγ type was found to be more common than δβγ. ADH increases the sodium transport across the frog skin. The effect of ADH on sodium transport is achieved through the combination of δ-subunits, not through the combination of a-subunits in the skin of Pana hexadactyla.",
author = "Jared, {Silviya Rajakumari} and Rao, {Jonakuty Prakasa}",
year = "2013",
month = "5",
day = "1",
doi = "10.2108/zsj.30.402",
language = "English",
volume = "30",
pages = "402--407",
journal = "Zoological Science",
issn = "0289-0003",
publisher = "Zoological Society of Japan",
number = "5",

}

Effects of evans blue and amiloride on anti-diuretic hormone (ADH)-induced sodium transport across frog (Rana hexadactyla) Skin. / Jared, Silviya Rajakumari; Rao, Jonakuty Prakasa.

In: Zoological Science, Vol. 30, No. 5, 01.05.2013, p. 402-407.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of evans blue and amiloride on anti-diuretic hormone (ADH)-induced sodium transport across frog (Rana hexadactyla) Skin

AU - Jared, Silviya Rajakumari

AU - Rao, Jonakuty Prakasa

PY - 2013/5/1

Y1 - 2013/5/1

N2 - The epithelial sodium channel (ENaC) has four subunits, namely α (alpha), β (beta), γ (gamma) and δ (delta). The functional ENaC is formed by the combination of either αβγ or δβγ subunits. The aim of the present study is to determine the combination of ENaC subunits predominant on the apical side of the frog skin, and the effect of ADH on sodium transport though these two ENaCs subunit combinations. The ventral abdominal skin of the frog, Rana hexadactyla was mounted in an Ussing-type chamber. The voltage-clamp method was performed to measure the ionic transport across the frog skin with normal Ringer solution (NR) on both sides. Evans blue (300 M) and amiloride (100 M) were added to the NR on the apical side and ADH (40 nM) was added on the serosal side. Statistical significance was analyzed by Student's paired t-test and repeated-measures ANOVA, P < 0.05 was considered significant. This study suggests that the ENaC of the frog skin consist of both αβγ and δβγ subunit combinations on the apical side. Though both types of subunit combination are present, the αβγ type was found to be more common than δβγ. ADH increases the sodium transport across the frog skin. The effect of ADH on sodium transport is achieved through the combination of δ-subunits, not through the combination of a-subunits in the skin of Pana hexadactyla.

AB - The epithelial sodium channel (ENaC) has four subunits, namely α (alpha), β (beta), γ (gamma) and δ (delta). The functional ENaC is formed by the combination of either αβγ or δβγ subunits. The aim of the present study is to determine the combination of ENaC subunits predominant on the apical side of the frog skin, and the effect of ADH on sodium transport though these two ENaCs subunit combinations. The ventral abdominal skin of the frog, Rana hexadactyla was mounted in an Ussing-type chamber. The voltage-clamp method was performed to measure the ionic transport across the frog skin with normal Ringer solution (NR) on both sides. Evans blue (300 M) and amiloride (100 M) were added to the NR on the apical side and ADH (40 nM) was added on the serosal side. Statistical significance was analyzed by Student's paired t-test and repeated-measures ANOVA, P < 0.05 was considered significant. This study suggests that the ENaC of the frog skin consist of both αβγ and δβγ subunit combinations on the apical side. Though both types of subunit combination are present, the αβγ type was found to be more common than δβγ. ADH increases the sodium transport across the frog skin. The effect of ADH on sodium transport is achieved through the combination of δ-subunits, not through the combination of a-subunits in the skin of Pana hexadactyla.

UR - http://www.scopus.com/inward/record.url?scp=84877614473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877614473&partnerID=8YFLogxK

U2 - 10.2108/zsj.30.402

DO - 10.2108/zsj.30.402

M3 - Article

VL - 30

SP - 402

EP - 407

JO - Zoological Science

JF - Zoological Science

SN - 0289-0003

IS - 5

ER -