Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells

Keiji Matsuda, Shin Ya Nakagawa, Tomoya Nakano, Jun Ichi Asaumi, Ganesh Chandra Jagetia, Shoji Kawasaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. Methods: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. Results: Heating of HSC4 cells at 45°C for 20 min and KB cells for 3min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 μM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. Conclusions: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.

Original languageEnglish
Pages (from-to)729-735
Number of pages7
JournalInternational Journal of Hyperthermia
Volume22
Issue number8
DOIs
Publication statusPublished - 01-12-2006
Externally publishedYes

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Histones
Methylation
Squamous Cell Carcinoma
Hot Temperature
Lysine
KB Cells
Heating
Schizosaccharomyces
Mouth Neoplasms
KNK 437
Genes
Western Blotting
Cell Line

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)
  • Cancer Research

Cite this

Matsuda, Keiji ; Nakagawa, Shin Ya ; Nakano, Tomoya ; Asaumi, Jun Ichi ; Jagetia, Ganesh Chandra ; Kawasaki, Shoji. / Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells. In: International Journal of Hyperthermia. 2006 ; Vol. 22, No. 8. pp. 729-735.
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Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells. / Matsuda, Keiji; Nakagawa, Shin Ya; Nakano, Tomoya; Asaumi, Jun Ichi; Jagetia, Ganesh Chandra; Kawasaki, Shoji.

In: International Journal of Hyperthermia, Vol. 22, No. 8, 01.12.2006, p. 729-735.

Research output: Contribution to journalArticle

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AU - Nakagawa, Shin Ya

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AU - Jagetia, Ganesh Chandra

AU - Kawasaki, Shoji

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N2 - Purpose: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. Methods: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. Results: Heating of HSC4 cells at 45°C for 20 min and KB cells for 3min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 μM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. Conclusions: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.

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