TY - JOUR
T1 - Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells
AU - Matsuda, Keiji
AU - Nakagawa, Shin Ya
AU - Nakano, Tomoya
AU - Asaumi, Jun Ichi
AU - Jagetia, Ganesh Chandra
AU - Kawasaki, Shoji
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Purpose: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. Methods: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. Results: Heating of HSC4 cells at 45°C for 20 min and KB cells for 3min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 μM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. Conclusions: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.
AB - Purpose: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. Methods: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. Results: Heating of HSC4 cells at 45°C for 20 min and KB cells for 3min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 μM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. Conclusions: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.
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U2 - 10.1080/02656730601074375
DO - 10.1080/02656730601074375
M3 - Article
C2 - 17391001
AN - SCOPUS:33847383673
SN - 0265-6736
VL - 22
SP - 729
EP - 735
JO - International Journal of Hyperthermia
JF - International Journal of Hyperthermia
IS - 8
ER -
