Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells

Keiji Matsuda, Shin Ya Nakagawa, Tomoya Nakano, Jun Ichi Asaumi, Ganesh Chandra Jagetia, Shoji Kawasaki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. Methods: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. Results: Heating of HSC4 cells at 45°C for 20 min and KB cells for 3min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 μM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. Conclusions: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.

Original languageEnglish
Pages (from-to)729-735
Number of pages7
JournalInternational Journal of Hyperthermia
Volume22
Issue number8
DOIs
Publication statusPublished - 01-12-2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)
  • Cancer Research

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