Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut

A. Muthusamy, K. Vasanth, D. Sivasankari, B. R. Chandrasekar, N. Jayabalan

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Abstract

The embryogenic calli (EC) were obtained from hypocotyl explants of groundnut (Arachis hypogaea L.) cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 0.5 mg dm-3 6-benzylaminopurine (BAP). The EC were exposed to γ-radiation (10-50 Gy) or treated with 1-5 mM of ethyl methane sulphonate (EMS) or sodium azide (SA). The mutated EC were subcultured on embryo induction medium containing 20 mg dm-3 2,4-D. Somatic embryos (SE) developed from these calli were transferred to MS medium supplemented with BAP (2.0 mg dm-3) and 0.5 mg dm-3 2,4-D for maturation. The well-developed embryos were cultured on germination medium consisting of MS salts with 2.0 mg dm-3 BAP and 0.25 mg dm -3 naphthaleneacetic acid (NAA). Well-developed plantlets were transferred for hardening and hardened plants produced normal flowers and set viable seeds. The fresh mass of the EC, mean number of SE per explant and regeneration percentage were higher at lower concentrations of mutagens (up to 30 Gy/3 mM). Some abnormalities in regenerated plants were observed, especially variations in leaf shape.

Original languageEnglish
Pages (from-to)430-435
Number of pages6
JournalBiologia Plantarum
Volume51
Issue number3
DOIs
Publication statusPublished - 09-2007

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somatic embryogenesis
peanuts
callus
2,4-D
somatic embryos
embryo (plant)
explants
ethyl methanesulfonate
sodium azide
Arachis hypogaea
naphthaleneacetic acid
benzyladenine
hypocotyls
plantlets
germination
salts
flowers
seeds
leaves

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Horticulture

Cite this

Muthusamy, A. ; Vasanth, K. ; Sivasankari, D. ; Chandrasekar, B. R. ; Jayabalan, N. / Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut. In: Biologia Plantarum. 2007 ; Vol. 51, No. 3. pp. 430-435.
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Muthusamy, A, Vasanth, K, Sivasankari, D, Chandrasekar, BR & Jayabalan, N 2007, 'Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut', Biologia Plantarum, vol. 51, no. 3, pp. 430-435. https://doi.org/10.1007/s10535-007-0092-y

Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut. / Muthusamy, A.; Vasanth, K.; Sivasankari, D.; Chandrasekar, B. R.; Jayabalan, N.

In: Biologia Plantarum, Vol. 51, No. 3, 09.2007, p. 430-435.

Research output: Contribution to journalArticle

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T1 - Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut

AU - Muthusamy, A.

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AU - Sivasankari, D.

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