Effects of Sardinella longiceps on the antioxidant parameters of cholesterol stressed rats during copper overload

M. Nandini, S. N. Rao, M. A. Rahiman

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The object of this study was to investigate the alteration in the antioxidant defense potential of cholesterol stressed rats supplemented with sardine fish (Sardinella longiceps) on isocaloric basis during copper overload. Male albino rats divided into 4 groups of seven each were fed the following diets for a period of 3 weeks. Basal diet (A), Basal diet + fish (B), Basal diet +cholesterol (C), Basal diet + cholesterol + fish (D). Copper loading was done by intraperitoneal injection of CuSO4 for a period of 4 days. Similar groups of animals without copper administration served as the control. Copper administration significantly increase the susceptibility of RBC to lipid peroxidation in the control group of rats. A significant decrease in the susceptibility to lipid peroxidation was observed in fish supplemented (B) and cholesterol stressed (C) rats during copper overload. A significant increase in the glutathione (GSH) status was noted in the control animals (A), but a decrease was observed in the cholesterol stressed group(C). Significant increase in the activity of glutathione peroxidase(GSH-px) was observed in the control (A) and cholesterol stressed (C) rats, and increase in glutathione reductase (GSSG-R) only in the control group of rats. Fish supplementation however did not show any significant change. No statistically significant differences in the RBC catalase and superoxide dismutase (SOD) activities were observed during copper overload. It is concluded that dietary fish, during copper overload potentially affects the dynamics of the antioxidant system.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalBiomedical Research
Issue number2
Publication statusPublished - 01-01-2001

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)


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