The increase of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) demands the search for alternative antimycobacterial drugs. The aim of this study was to evaluate plants used in Indian traditional medicine to treat respiratory diseases for activity against MDR-TB. Six plants(Taxus baccata, Senna alata, Andrographis paniculata, Adhatoda vasica Nees, Acalypha indica L., Aloe vera L.) was selected for activity against Mycobacterium tuberculosis and aqueous extracts of these leaves at concentrations from 2%,4%,6% were tested in vitro for their activity against one MDR isolate (DKU-156), reference susceptible strain M. tuberculosis H37Rv as well as rapid grower mycobacterial pathogen M. fortuitum (TMC-1529) using Lowenstein Jensen (L-J) medium. Activity in L-J medium was evaluated by percentage inhibition which was calculated by mean reduction in number of colonies on extract containing as compared to extract free controls. Extracts of all the six plants Taxus baccata, Senna alata, Andrographis paniculata , Adhatoda vasica Nees, Acalypha indica L., Aloe vera L exhibited anti-tuberculosis activity in L-J medium, the proportion of inhibition of these plants extract in respect mentioned above is 100,32,46,43,75 51 per cent, respectively for MDR isolate DKU-156, while for M. tuberculosis H37Rv, inhibition was found to be 86,46,40,71,69, 51 per cent,and 66,0,0,0,33,33 percent against rapid grower M. fortuitum (TMC-1529) at 6 per cent concentration in L-J medium. All these plant extract showed promising quantifiable antimicrobial activity against MDR Mycobacterium tuberculosis (DKU-156), and can be used for future ethano-pharmacological studies and drug development.
|Number of pages||6|
|Journal||Research Journal of Pharmaceutical, Biological and Chemical Sciences|
|Publication status||Published - 01-07-2013|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)