Electro-kinetically driven route for highly sensitive blood pathology on a paper-based device

Naresh Kumar Mani, Sankha Shuvra Das, Sayantan Dawn, Suman Chakraborty

Research output: Contribution to journalArticle

Abstract

Enhancing the sensitivity of colorimetric detection in paper-devices is a quintessential step in achieving frugal diagnosis. Here, we demonstrate an effective way of improving the detection sensitivity of paper-based devices, as mediated by electro-kinetic mechanisms. By directly employing blood plasma, we investigate the electro-kinetic clustering of glucose, a neutral molecule in paper devices. Under the influence of uniform electric field, dispersed glucose gets accumulated in the paper strips. Due to the combination of EOF and electrophoretic migration, we achieve twofold increase in the colour intensity for both normal and diabetic samples. This approach is robust and possesses better sensitivity than conventional colorimetric assays and can be easily extended to other body fluid based diagnosis. These results may turn out to be of profound importance in improving the quality of pathological diagnosis in low-cost paper-based point-of-care devices deployed in resource-limited settings.

Original languageEnglish
JournalElectrophoresis
DOIs
Publication statusAccepted/In press - 01-01-2019

Fingerprint

Pathology
Blood
Equipment and Supplies
Glucose
Kinetics
Body fluids
Point-of-Care Systems
Assays
Body Fluids
Electric fields
Color
Cluster Analysis
Plasmas
Molecules
Costs and Cost Analysis
Costs

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

@article{ad8538b6e85d4251823de62668840f5c,
title = "Electro-kinetically driven route for highly sensitive blood pathology on a paper-based device",
abstract = "Enhancing the sensitivity of colorimetric detection in paper-devices is a quintessential step in achieving frugal diagnosis. Here, we demonstrate an effective way of improving the detection sensitivity of paper-based devices, as mediated by electro-kinetic mechanisms. By directly employing blood plasma, we investigate the electro-kinetic clustering of glucose, a neutral molecule in paper devices. Under the influence of uniform electric field, dispersed glucose gets accumulated in the paper strips. Due to the combination of EOF and electrophoretic migration, we achieve twofold increase in the colour intensity for both normal and diabetic samples. This approach is robust and possesses better sensitivity than conventional colorimetric assays and can be easily extended to other body fluid based diagnosis. These results may turn out to be of profound importance in improving the quality of pathological diagnosis in low-cost paper-based point-of-care devices deployed in resource-limited settings.",
author = "Mani, {Naresh Kumar} and Das, {Sankha Shuvra} and Sayantan Dawn and Suman Chakraborty",
year = "2019",
month = "1",
day = "1",
doi = "10.1002/elps.201900356",
language = "English",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",

}

Electro-kinetically driven route for highly sensitive blood pathology on a paper-based device. / Mani, Naresh Kumar; Das, Sankha Shuvra; Dawn, Sayantan; Chakraborty, Suman.

In: Electrophoresis, 01.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Electro-kinetically driven route for highly sensitive blood pathology on a paper-based device

AU - Mani, Naresh Kumar

AU - Das, Sankha Shuvra

AU - Dawn, Sayantan

AU - Chakraborty, Suman

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Enhancing the sensitivity of colorimetric detection in paper-devices is a quintessential step in achieving frugal diagnosis. Here, we demonstrate an effective way of improving the detection sensitivity of paper-based devices, as mediated by electro-kinetic mechanisms. By directly employing blood plasma, we investigate the electro-kinetic clustering of glucose, a neutral molecule in paper devices. Under the influence of uniform electric field, dispersed glucose gets accumulated in the paper strips. Due to the combination of EOF and electrophoretic migration, we achieve twofold increase in the colour intensity for both normal and diabetic samples. This approach is robust and possesses better sensitivity than conventional colorimetric assays and can be easily extended to other body fluid based diagnosis. These results may turn out to be of profound importance in improving the quality of pathological diagnosis in low-cost paper-based point-of-care devices deployed in resource-limited settings.

AB - Enhancing the sensitivity of colorimetric detection in paper-devices is a quintessential step in achieving frugal diagnosis. Here, we demonstrate an effective way of improving the detection sensitivity of paper-based devices, as mediated by electro-kinetic mechanisms. By directly employing blood plasma, we investigate the electro-kinetic clustering of glucose, a neutral molecule in paper devices. Under the influence of uniform electric field, dispersed glucose gets accumulated in the paper strips. Due to the combination of EOF and electrophoretic migration, we achieve twofold increase in the colour intensity for both normal and diabetic samples. This approach is robust and possesses better sensitivity than conventional colorimetric assays and can be easily extended to other body fluid based diagnosis. These results may turn out to be of profound importance in improving the quality of pathological diagnosis in low-cost paper-based point-of-care devices deployed in resource-limited settings.

UR - http://www.scopus.com/inward/record.url?scp=85077900629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85077900629&partnerID=8YFLogxK

U2 - 10.1002/elps.201900356

DO - 10.1002/elps.201900356

M3 - Article

C2 - 31891191

AN - SCOPUS:85077900629

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

ER -