Enhanced production of poly(3-hydroxybutyrate) in a novel airlift reactor with in situ cell retention using Azohydromonas australica

Geeta Gahlawat, Bedoshree Sengupta, Ashok K. Srivastava

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Economic production of biodegradable plastics is a challenge particularly because of high substrate and energy cost inputs for its production. Research efforts are being directed towards innovations to minimize both of the above costs to economize polyhydroxybutyrate (PHB) production. A novel airlift reactor (ALR) with outer aeration and internal settling was utilized in this investigation. Although it featured no power consumption for agitation, it facilitated increased oxygen transfer rate and better cell retention than stirred tank reactor (STR), thereby resulting in enhancedPHB productivity. ALR with in situ cell retention demonstrated a significant improvement in biomass concentration and biopolymer accumulation. The total PHB production rate, specific biomass, and product yield in the ALR were observed to be 0.84 g/h, 0.43 g/g, and 0.32 g/g, respectively. The studies revealed that the volumetric oxygen mass transfer rate and mixing time for ALR were 0.016 s-1 and 3.73 s, respectively, at 2.0 vvm as compared with corresponding values of 0.005 s-1 and 4.95 s, respectively, in STR. This demonstrated that ALR has better oxygen mass transfer and mixing efficiency than STR. Hence, ALR with cell retention would serve as a better bioreactor design for economic biopolymer production than STR, particularly due to its lower cost of operation and simplicity along with its enhanced oxygen and heat transfer rates.

Original languageEnglish
Pages (from-to)1377-1384
Number of pages8
JournalJournal of Industrial Microbiology and Biotechnology
Volume39
Issue number9
DOIs
Publication statusPublished - 01-09-2012
Externally publishedYes

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Oxygen
Biopolymers
Costs and Cost Analysis
Biomass
Biodegradable Plastics
Economics
Mass transfer
Bioreactors
Costs
Hot Temperature
Electric power utilization
Innovation
Productivity
poly-beta-hydroxybutyrate
Plastics
Heat transfer
Research
Substrates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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Enhanced production of poly(3-hydroxybutyrate) in a novel airlift reactor with in situ cell retention using Azohydromonas australica. / Gahlawat, Geeta; Sengupta, Bedoshree; Srivastava, Ashok K.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 39, No. 9, 01.09.2012, p. 1377-1384.

Research output: Contribution to journalArticle

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