Abstract

Objective: The altered miRNAs expression in cervical cancer tissue can be a critical player during tumorigenesis, may contribute to tumor cell heterogeneity and may determine distinct phenotypes within the tumor. Recent studies have highlighted the role of circulating miRNAs as a minimally-invasive biomarker and its potential as biosignature to complement routine tissue-based procedures. Methods: In order to determine whether miRNAs in serum can indicate changes in cervical tissue specimens, we performed small RNA sequencing and selected miRNAs were validated using qRT-PCR in serum and tissue specimens (n = 115). Further, luciferase assay were performed to investigate the interactions between hsa-miR-409-3p and hsa-miR-454-3p binding sites on 3′UTR region of MTF2 and ST18 respectively. Results: We have identified a total of 14 differentially expressed miRNAs common in serum and tissue specimens. Among them, hsa-miR-17-5p, hsa-miR-32-5p and hsa-miR-454-3p were upregulated while, hsa-miR-409-3p was downregulated in serum and tissue of cervical cancer subjects. Our in-silico small RNA sequencing data analysis identified isomiRs and classified miRNA into clusters and subtypes (exonic, intronic and intergenic) with respect to the expression status in serum and tissue specimens. Expression level of hsa-miR-409-3p and hsa-miR-454-3p were inversely correlated with their target genes MTF2 and ST18 levels respectively in human cervical cancer specimens. Luciferase assay demonstrated that hsa-miR-409-3p and hsa-miR-454-3p functionally interacts with 3′-UTR of MTF2 and ST18 respectively to decrease their activity. Conclusion: Our results support the significant role of circulating miRNAs in disease dissemination and their potential utility as biosignatures of clinical relevance.

Original languageEnglish
JournalGynecologic Oncology
DOIs
Publication statusAccepted/In press - 01-01-2019

Fingerprint

MicroRNAs
Uterine Cervical Neoplasms
Biopsy
RNA Sequence Analysis
Serum
Luciferases
3' Untranslated Regions
Computer Simulation
Neoplasms
Carcinogenesis
Down-Regulation
Biomarkers
Binding Sites
Phenotype
Polymerase Chain Reaction
human MIRN409 microRNA
human MIRN454 microRNA
Genes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Obstetrics and Gynaecology

Cite this

@article{64129c78fa3f4898800569f1420078cd,
title = "Enumeration of deregulated miRNAs in liquid and tissue biopsies of cervical cancer",
abstract = "Objective: The altered miRNAs expression in cervical cancer tissue can be a critical player during tumorigenesis, may contribute to tumor cell heterogeneity and may determine distinct phenotypes within the tumor. Recent studies have highlighted the role of circulating miRNAs as a minimally-invasive biomarker and its potential as biosignature to complement routine tissue-based procedures. Methods: In order to determine whether miRNAs in serum can indicate changes in cervical tissue specimens, we performed small RNA sequencing and selected miRNAs were validated using qRT-PCR in serum and tissue specimens (n = 115). Further, luciferase assay were performed to investigate the interactions between hsa-miR-409-3p and hsa-miR-454-3p binding sites on 3′UTR region of MTF2 and ST18 respectively. Results: We have identified a total of 14 differentially expressed miRNAs common in serum and tissue specimens. Among them, hsa-miR-17-5p, hsa-miR-32-5p and hsa-miR-454-3p were upregulated while, hsa-miR-409-3p was downregulated in serum and tissue of cervical cancer subjects. Our in-silico small RNA sequencing data analysis identified isomiRs and classified miRNA into clusters and subtypes (exonic, intronic and intergenic) with respect to the expression status in serum and tissue specimens. Expression level of hsa-miR-409-3p and hsa-miR-454-3p were inversely correlated with their target genes MTF2 and ST18 levels respectively in human cervical cancer specimens. Luciferase assay demonstrated that hsa-miR-409-3p and hsa-miR-454-3p functionally interacts with 3′-UTR of MTF2 and ST18 respectively to decrease their activity. Conclusion: Our results support the significant role of circulating miRNAs in disease dissemination and their potential utility as biosignatures of clinical relevance.",
author = "Vaibhav Shukla and Varghese, {Vinay Koshy} and Kabekkodu, {Shama Prasada} and Sandeep Mallya and Sanjiban Chakrabarty and Pradyumna Jayaram and Deeksha Pandey and Sourjya Banerjee and Krishna Sharan and Kapaettu Satyamoorthy",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.ygyno.2019.08.012",
language = "English",
journal = "Gynecologic Oncology",
issn = "0090-8258",
publisher = "Academic Press Inc.",

}

Enumeration of deregulated miRNAs in liquid and tissue biopsies of cervical cancer. / Shukla, Vaibhav; Varghese, Vinay Koshy; Kabekkodu, Shama Prasada; Mallya, Sandeep; Chakrabarty, Sanjiban; Jayaram, Pradyumna; Pandey, Deeksha; Banerjee, Sourjya; Sharan, Krishna; Satyamoorthy, Kapaettu.

In: Gynecologic Oncology, 01.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enumeration of deregulated miRNAs in liquid and tissue biopsies of cervical cancer

AU - Shukla, Vaibhav

AU - Varghese, Vinay Koshy

AU - Kabekkodu, Shama Prasada

AU - Mallya, Sandeep

AU - Chakrabarty, Sanjiban

AU - Jayaram, Pradyumna

AU - Pandey, Deeksha

AU - Banerjee, Sourjya

AU - Sharan, Krishna

AU - Satyamoorthy, Kapaettu

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objective: The altered miRNAs expression in cervical cancer tissue can be a critical player during tumorigenesis, may contribute to tumor cell heterogeneity and may determine distinct phenotypes within the tumor. Recent studies have highlighted the role of circulating miRNAs as a minimally-invasive biomarker and its potential as biosignature to complement routine tissue-based procedures. Methods: In order to determine whether miRNAs in serum can indicate changes in cervical tissue specimens, we performed small RNA sequencing and selected miRNAs were validated using qRT-PCR in serum and tissue specimens (n = 115). Further, luciferase assay were performed to investigate the interactions between hsa-miR-409-3p and hsa-miR-454-3p binding sites on 3′UTR region of MTF2 and ST18 respectively. Results: We have identified a total of 14 differentially expressed miRNAs common in serum and tissue specimens. Among them, hsa-miR-17-5p, hsa-miR-32-5p and hsa-miR-454-3p were upregulated while, hsa-miR-409-3p was downregulated in serum and tissue of cervical cancer subjects. Our in-silico small RNA sequencing data analysis identified isomiRs and classified miRNA into clusters and subtypes (exonic, intronic and intergenic) with respect to the expression status in serum and tissue specimens. Expression level of hsa-miR-409-3p and hsa-miR-454-3p were inversely correlated with their target genes MTF2 and ST18 levels respectively in human cervical cancer specimens. Luciferase assay demonstrated that hsa-miR-409-3p and hsa-miR-454-3p functionally interacts with 3′-UTR of MTF2 and ST18 respectively to decrease their activity. Conclusion: Our results support the significant role of circulating miRNAs in disease dissemination and their potential utility as biosignatures of clinical relevance.

AB - Objective: The altered miRNAs expression in cervical cancer tissue can be a critical player during tumorigenesis, may contribute to tumor cell heterogeneity and may determine distinct phenotypes within the tumor. Recent studies have highlighted the role of circulating miRNAs as a minimally-invasive biomarker and its potential as biosignature to complement routine tissue-based procedures. Methods: In order to determine whether miRNAs in serum can indicate changes in cervical tissue specimens, we performed small RNA sequencing and selected miRNAs were validated using qRT-PCR in serum and tissue specimens (n = 115). Further, luciferase assay were performed to investigate the interactions between hsa-miR-409-3p and hsa-miR-454-3p binding sites on 3′UTR region of MTF2 and ST18 respectively. Results: We have identified a total of 14 differentially expressed miRNAs common in serum and tissue specimens. Among them, hsa-miR-17-5p, hsa-miR-32-5p and hsa-miR-454-3p were upregulated while, hsa-miR-409-3p was downregulated in serum and tissue of cervical cancer subjects. Our in-silico small RNA sequencing data analysis identified isomiRs and classified miRNA into clusters and subtypes (exonic, intronic and intergenic) with respect to the expression status in serum and tissue specimens. Expression level of hsa-miR-409-3p and hsa-miR-454-3p were inversely correlated with their target genes MTF2 and ST18 levels respectively in human cervical cancer specimens. Luciferase assay demonstrated that hsa-miR-409-3p and hsa-miR-454-3p functionally interacts with 3′-UTR of MTF2 and ST18 respectively to decrease their activity. Conclusion: Our results support the significant role of circulating miRNAs in disease dissemination and their potential utility as biosignatures of clinical relevance.

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JF - Gynecologic Oncology

SN - 0090-8258

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