Epidemiological study of Vibrio cholerae using variable number of tandem repeats

Raikamal Ghosh, G. Balakrish Nair, Li Tang, J. Glenn Morris, Naresh C. Sharma, Mamatha Ballal, Pallavi Garg, Thandavarayan Ramamurthy, O. Colin Stine

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

By conventional genetic methods, including pulse-field gel electrophoresis and multilocus sequence typing, most pathogenic, cholera toxin-positive O1 and O139 isolates of Vibrio cholerae cannot be distinguished. We evaluated relationships among 173 V. cholerae isolates collected between 1992 and 2007 from different geographic areas in India by analyzing five variable number of tandem repeat (VNTR) loci. Each VNTR locus was highly variable, with between 5 and 19 alleles. eburst analysis revealed four large groups of genetically related isolates. Two groups contained genotypes of isolates with the O139 serogroup (which emerged for the first time in epidemic form in 1992), with the other two groups containing O1 strains. In subsequent analysis, it was possible to track the spread of specific genotypes across time and space. Our data highlight the utility of the methodology as an epidemiologic tool for assessing spread of isolates in both epidemic and endemic settings.

Original languageEnglish
Pages (from-to)196-201
Number of pages6
JournalFEMS Microbiology Letters
Volume288
Issue number2
DOIs
Publication statusPublished - 01-11-2008

Fingerprint

Minisatellite Repeats
Vibrio cholerae
Epidemiologic Studies
Vibrio cholerae O139
Genotype
Multilocus Sequence Typing
Cholera Toxin
Electrophoresis
India
Gels
Alleles
Serogroup

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Ghosh, R., Nair, G. B., Tang, L., Morris, J. G., Sharma, N. C., Ballal, M., ... Stine, O. C. (2008). Epidemiological study of Vibrio cholerae using variable number of tandem repeats. FEMS Microbiology Letters, 288(2), 196-201. https://doi.org/10.1111/j.1574-6968.2008.01352.x
Ghosh, Raikamal ; Nair, G. Balakrish ; Tang, Li ; Morris, J. Glenn ; Sharma, Naresh C. ; Ballal, Mamatha ; Garg, Pallavi ; Ramamurthy, Thandavarayan ; Stine, O. Colin. / Epidemiological study of Vibrio cholerae using variable number of tandem repeats. In: FEMS Microbiology Letters. 2008 ; Vol. 288, No. 2. pp. 196-201.
@article{cb9614ee095d4678b9595ea124a7bb2e,
title = "Epidemiological study of Vibrio cholerae using variable number of tandem repeats",
abstract = "By conventional genetic methods, including pulse-field gel electrophoresis and multilocus sequence typing, most pathogenic, cholera toxin-positive O1 and O139 isolates of Vibrio cholerae cannot be distinguished. We evaluated relationships among 173 V. cholerae isolates collected between 1992 and 2007 from different geographic areas in India by analyzing five variable number of tandem repeat (VNTR) loci. Each VNTR locus was highly variable, with between 5 and 19 alleles. eburst analysis revealed four large groups of genetically related isolates. Two groups contained genotypes of isolates with the O139 serogroup (which emerged for the first time in epidemic form in 1992), with the other two groups containing O1 strains. In subsequent analysis, it was possible to track the spread of specific genotypes across time and space. Our data highlight the utility of the methodology as an epidemiologic tool for assessing spread of isolates in both epidemic and endemic settings.",
author = "Raikamal Ghosh and Nair, {G. Balakrish} and Li Tang and Morris, {J. Glenn} and Sharma, {Naresh C.} and Mamatha Ballal and Pallavi Garg and Thandavarayan Ramamurthy and Stine, {O. Colin}",
year = "2008",
month = "11",
day = "1",
doi = "10.1111/j.1574-6968.2008.01352.x",
language = "English",
volume = "288",
pages = "196--201",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2",

}

Ghosh, R, Nair, GB, Tang, L, Morris, JG, Sharma, NC, Ballal, M, Garg, P, Ramamurthy, T & Stine, OC 2008, 'Epidemiological study of Vibrio cholerae using variable number of tandem repeats', FEMS Microbiology Letters, vol. 288, no. 2, pp. 196-201. https://doi.org/10.1111/j.1574-6968.2008.01352.x

Epidemiological study of Vibrio cholerae using variable number of tandem repeats. / Ghosh, Raikamal; Nair, G. Balakrish; Tang, Li; Morris, J. Glenn; Sharma, Naresh C.; Ballal, Mamatha; Garg, Pallavi; Ramamurthy, Thandavarayan; Stine, O. Colin.

In: FEMS Microbiology Letters, Vol. 288, No. 2, 01.11.2008, p. 196-201.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Epidemiological study of Vibrio cholerae using variable number of tandem repeats

AU - Ghosh, Raikamal

AU - Nair, G. Balakrish

AU - Tang, Li

AU - Morris, J. Glenn

AU - Sharma, Naresh C.

AU - Ballal, Mamatha

AU - Garg, Pallavi

AU - Ramamurthy, Thandavarayan

AU - Stine, O. Colin

PY - 2008/11/1

Y1 - 2008/11/1

N2 - By conventional genetic methods, including pulse-field gel electrophoresis and multilocus sequence typing, most pathogenic, cholera toxin-positive O1 and O139 isolates of Vibrio cholerae cannot be distinguished. We evaluated relationships among 173 V. cholerae isolates collected between 1992 and 2007 from different geographic areas in India by analyzing five variable number of tandem repeat (VNTR) loci. Each VNTR locus was highly variable, with between 5 and 19 alleles. eburst analysis revealed four large groups of genetically related isolates. Two groups contained genotypes of isolates with the O139 serogroup (which emerged for the first time in epidemic form in 1992), with the other two groups containing O1 strains. In subsequent analysis, it was possible to track the spread of specific genotypes across time and space. Our data highlight the utility of the methodology as an epidemiologic tool for assessing spread of isolates in both epidemic and endemic settings.

AB - By conventional genetic methods, including pulse-field gel electrophoresis and multilocus sequence typing, most pathogenic, cholera toxin-positive O1 and O139 isolates of Vibrio cholerae cannot be distinguished. We evaluated relationships among 173 V. cholerae isolates collected between 1992 and 2007 from different geographic areas in India by analyzing five variable number of tandem repeat (VNTR) loci. Each VNTR locus was highly variable, with between 5 and 19 alleles. eburst analysis revealed four large groups of genetically related isolates. Two groups contained genotypes of isolates with the O139 serogroup (which emerged for the first time in epidemic form in 1992), with the other two groups containing O1 strains. In subsequent analysis, it was possible to track the spread of specific genotypes across time and space. Our data highlight the utility of the methodology as an epidemiologic tool for assessing spread of isolates in both epidemic and endemic settings.

UR - http://www.scopus.com/inward/record.url?scp=53849124343&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53849124343&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.2008.01352.x

DO - 10.1111/j.1574-6968.2008.01352.x

M3 - Article

C2 - 18811655

AN - SCOPUS:53849124343

VL - 288

SP - 196

EP - 201

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -