Abstract
Metastatic spread is the major cause of breast cancer deaths. The tumor associated lymphatics have long been believed to be the main route for early spread of tumor cells. In the present study polybutylcyanoacrylate (PBCA) nanoparticles containing Epirubicin Hydrochloride (EPI) were synthesized by anionic polymerization in acidic medium in the presence of Dextran 40, Poloxamer 407 and Poloxamine 904. The influence of stabilizer concentration and EPI loading on particle size and entrapment efficiency were studied. The in-vitro drug release study was carried out in phosphate buffer saline pH 7.4 at 37°C. The optimum particle size and entrapment efficiency was observed at 0.05% w/v EPI loading, at pH 2.5 and at 1% monomer and surfactant concentration. The in-vitro release of EPI from all the EPI-PBCA nanoparticles in phosphate-buffered saline pH 7.4 at 37°C showed biphasic release with an initial burst release followed by sustained release phase of EPI from nanoparticles. In-vitro cell studies were carried out in human breast cancer cell lines MCF-7 and MDA-MB-231and the results revealed similar cytotoxicity when compared with free drug in the breast cancer cells evaluated.
| Original language | English |
|---|---|
| Title of host publication | Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 |
| Pages | 36-39 |
| Number of pages | 4 |
| Volume | 2 |
| Publication status | Published - 2009 |
| Event | Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, United States Duration: 03-05-2009 → 07-05-2009 |
Conference
| Conference | Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 |
|---|---|
| Country | United States |
| City | Houston, TX |
| Period | 03-05-09 → 07-05-09 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biotechnology
- Biomedical Engineering
- Biomaterials
Cite this
}
Epirubicin Hydrochloride loaded poly (butylcyanoacrylate) nanoparticles : Formulation optimization, characterization and in-vitro evaluation in human breast cancer cell lines. / Dantuluri, Prudhviraju; Shah, Neha; Kumar, M. R Sunil; Rao, B. S Satish; Murthy, R. S R.
Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009. Vol. 2 2009. p. 36-39.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Epirubicin Hydrochloride loaded poly (butylcyanoacrylate) nanoparticles
T2 - Formulation optimization, characterization and in-vitro evaluation in human breast cancer cell lines
AU - Dantuluri, Prudhviraju
AU - Shah, Neha
AU - Kumar, M. R Sunil
AU - Rao, B. S Satish
AU - Murthy, R. S R
PY - 2009
Y1 - 2009
N2 - Metastatic spread is the major cause of breast cancer deaths. The tumor associated lymphatics have long been believed to be the main route for early spread of tumor cells. In the present study polybutylcyanoacrylate (PBCA) nanoparticles containing Epirubicin Hydrochloride (EPI) were synthesized by anionic polymerization in acidic medium in the presence of Dextran 40, Poloxamer 407 and Poloxamine 904. The influence of stabilizer concentration and EPI loading on particle size and entrapment efficiency were studied. The in-vitro drug release study was carried out in phosphate buffer saline pH 7.4 at 37°C. The optimum particle size and entrapment efficiency was observed at 0.05% w/v EPI loading, at pH 2.5 and at 1% monomer and surfactant concentration. The in-vitro release of EPI from all the EPI-PBCA nanoparticles in phosphate-buffered saline pH 7.4 at 37°C showed biphasic release with an initial burst release followed by sustained release phase of EPI from nanoparticles. In-vitro cell studies were carried out in human breast cancer cell lines MCF-7 and MDA-MB-231and the results revealed similar cytotoxicity when compared with free drug in the breast cancer cells evaluated.
AB - Metastatic spread is the major cause of breast cancer deaths. The tumor associated lymphatics have long been believed to be the main route for early spread of tumor cells. In the present study polybutylcyanoacrylate (PBCA) nanoparticles containing Epirubicin Hydrochloride (EPI) were synthesized by anionic polymerization in acidic medium in the presence of Dextran 40, Poloxamer 407 and Poloxamine 904. The influence of stabilizer concentration and EPI loading on particle size and entrapment efficiency were studied. The in-vitro drug release study was carried out in phosphate buffer saline pH 7.4 at 37°C. The optimum particle size and entrapment efficiency was observed at 0.05% w/v EPI loading, at pH 2.5 and at 1% monomer and surfactant concentration. The in-vitro release of EPI from all the EPI-PBCA nanoparticles in phosphate-buffered saline pH 7.4 at 37°C showed biphasic release with an initial burst release followed by sustained release phase of EPI from nanoparticles. In-vitro cell studies were carried out in human breast cancer cell lines MCF-7 and MDA-MB-231and the results revealed similar cytotoxicity when compared with free drug in the breast cancer cells evaluated.
UR - http://www.scopus.com/inward/record.url?scp=77958049582&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77958049582&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:77958049582
SN - 9781439817834
SN - 9781439817834
VL - 2
SP - 36
EP - 39
BT - Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
ER -