Epirubicin Hydrochloride loaded poly (butylcyanoacrylate) nanoparticles: Formulation optimization, characterization and in-vitro evaluation in human breast cancer cell lines

Prudhviraju Dantuluri, Neha Shah, M. R Sunil Kumar, B. S Satish Rao, R. S R Murthy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Metastatic spread is the major cause of breast cancer deaths. The tumor associated lymphatics have long been believed to be the main route for early spread of tumor cells. In the present study polybutylcyanoacrylate (PBCA) nanoparticles containing Epirubicin Hydrochloride (EPI) were synthesized by anionic polymerization in acidic medium in the presence of Dextran 40, Poloxamer 407 and Poloxamine 904. The influence of stabilizer concentration and EPI loading on particle size and entrapment efficiency were studied. The in-vitro drug release study was carried out in phosphate buffer saline pH 7.4 at 37°C. The optimum particle size and entrapment efficiency was observed at 0.05% w/v EPI loading, at pH 2.5 and at 1% monomer and surfactant concentration. The in-vitro release of EPI from all the EPI-PBCA nanoparticles in phosphate-buffered saline pH 7.4 at 37°C showed biphasic release with an initial burst release followed by sustained release phase of EPI from nanoparticles. In-vitro cell studies were carried out in human breast cancer cell lines MCF-7 and MDA-MB-231and the results revealed similar cytotoxicity when compared with free drug in the breast cancer cells evaluated.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Pages36-39
Number of pages4
Volume2
Publication statusPublished - 2009
EventNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, United States
Duration: 03-05-200907-05-2009

Conference

ConferenceNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
CountryUnited States
CityHouston, TX
Period03-05-0907-05-09

Fingerprint

Enbucrilate
Epirubicin
Nanoparticles
Cells
Breast Neoplasms
Cell Line
Tumors
Phosphates
Particle size
Anionic polymerization
Dextran
Cytotoxicity
Particle Size
Surface active agents
Monomers
Poloxamer
Dextrans
Surface-Active Agents
Polymerization
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials

Cite this

Dantuluri, P., Shah, N., Kumar, M. R. S., Rao, B. S. S., & Murthy, R. S. R. (2009). Epirubicin Hydrochloride loaded poly (butylcyanoacrylate) nanoparticles: Formulation optimization, characterization and in-vitro evaluation in human breast cancer cell lines. In Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 (Vol. 2, pp. 36-39)
Dantuluri, Prudhviraju ; Shah, Neha ; Kumar, M. R Sunil ; Rao, B. S Satish ; Murthy, R. S R. / Epirubicin Hydrochloride loaded poly (butylcyanoacrylate) nanoparticles : Formulation optimization, characterization and in-vitro evaluation in human breast cancer cell lines. Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009. Vol. 2 2009. pp. 36-39
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abstract = "Metastatic spread is the major cause of breast cancer deaths. The tumor associated lymphatics have long been believed to be the main route for early spread of tumor cells. In the present study polybutylcyanoacrylate (PBCA) nanoparticles containing Epirubicin Hydrochloride (EPI) were synthesized by anionic polymerization in acidic medium in the presence of Dextran 40, Poloxamer 407 and Poloxamine 904. The influence of stabilizer concentration and EPI loading on particle size and entrapment efficiency were studied. The in-vitro drug release study was carried out in phosphate buffer saline pH 7.4 at 37°C. The optimum particle size and entrapment efficiency was observed at 0.05{\%} w/v EPI loading, at pH 2.5 and at 1{\%} monomer and surfactant concentration. The in-vitro release of EPI from all the EPI-PBCA nanoparticles in phosphate-buffered saline pH 7.4 at 37°C showed biphasic release with an initial burst release followed by sustained release phase of EPI from nanoparticles. In-vitro cell studies were carried out in human breast cancer cell lines MCF-7 and MDA-MB-231and the results revealed similar cytotoxicity when compared with free drug in the breast cancer cells evaluated.",
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Dantuluri, P, Shah, N, Kumar, MRS, Rao, BSS & Murthy, RSR 2009, Epirubicin Hydrochloride loaded poly (butylcyanoacrylate) nanoparticles: Formulation optimization, characterization and in-vitro evaluation in human breast cancer cell lines. in Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009. vol. 2, pp. 36-39, Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009, Houston, TX, United States, 03-05-09.

Epirubicin Hydrochloride loaded poly (butylcyanoacrylate) nanoparticles : Formulation optimization, characterization and in-vitro evaluation in human breast cancer cell lines. / Dantuluri, Prudhviraju; Shah, Neha; Kumar, M. R Sunil; Rao, B. S Satish; Murthy, R. S R.

Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009. Vol. 2 2009. p. 36-39.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - Metastatic spread is the major cause of breast cancer deaths. The tumor associated lymphatics have long been believed to be the main route for early spread of tumor cells. In the present study polybutylcyanoacrylate (PBCA) nanoparticles containing Epirubicin Hydrochloride (EPI) were synthesized by anionic polymerization in acidic medium in the presence of Dextran 40, Poloxamer 407 and Poloxamine 904. The influence of stabilizer concentration and EPI loading on particle size and entrapment efficiency were studied. The in-vitro drug release study was carried out in phosphate buffer saline pH 7.4 at 37°C. The optimum particle size and entrapment efficiency was observed at 0.05% w/v EPI loading, at pH 2.5 and at 1% monomer and surfactant concentration. The in-vitro release of EPI from all the EPI-PBCA nanoparticles in phosphate-buffered saline pH 7.4 at 37°C showed biphasic release with an initial burst release followed by sustained release phase of EPI from nanoparticles. In-vitro cell studies were carried out in human breast cancer cell lines MCF-7 and MDA-MB-231and the results revealed similar cytotoxicity when compared with free drug in the breast cancer cells evaluated.

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Dantuluri P, Shah N, Kumar MRS, Rao BSS, Murthy RSR. Epirubicin Hydrochloride loaded poly (butylcyanoacrylate) nanoparticles: Formulation optimization, characterization and in-vitro evaluation in human breast cancer cell lines. In Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009. Vol. 2. 2009. p. 36-39