Erythrocytes as carrier for prednisolone: In vitro andin vivo evaluation

G.V. Shavi, R.C. Doijad, P.B. Deshpande, F.V. Manvi, S.R. Meka, N. Udupa, R. Omprakash, K. Dhirendra

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Resealed erythrocytes, as drug delivery system has tremendous potential to achieve site specificity and prolonged release of drug thereby enhancing therapeutic index and patient compliance. In the present investigation erythrocytes obtained from healthy volunteers were loaded with prednisolone using preswell dilution and dilution technique with two different cross-linking agents, glutaraldehyde and dimethylsulphoxide. Carrier erythrocytes, having acceptable loading parameters showed increased percentage drug content with the addition of cross-linking agents. In vitro drug release followed zero-order kinetics, haemoglobin content was found to be satisfactory and osmotic fragility study indicated that increased drug entrapment efficiency was found at 0.3%w/v concentration of sodium chloride (hypotonic solution). In vivo tissue distribution studies were carried out for optimized formulation and order of distribution was found to be Liver>Lung>Kidney> Spleen. The developed drug delivery system is endowed with several exclusive advantages and hence holds potential for further research and clinical application.
Original languageEnglish
Pages (from-to)194-200
Number of pages7
JournalPakistan Journal of Pharmaceutical Sciences
Volume23
Issue number2
DOIs
Publication statusPublished - 04-2010

Fingerprint

Prednisolone
Erythrocytes
Drug Delivery Systems
Hypotonic Solutions
Osmotic Fragility
Indicator Dilution Techniques
Glutaral
Tissue Distribution
Patient Compliance
Dimethyl Sulfoxide
Sodium Chloride
Pharmaceutical Preparations
Healthy Volunteers
Hemoglobins
Spleen
Kidney
Lung
Liver
Research
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Shavi, G.V. ; Doijad, R.C. ; Deshpande, P.B. ; Manvi, F.V. ; Meka, S.R. ; Udupa, N. ; Omprakash, R. ; Dhirendra, K. / Erythrocytes as carrier for prednisolone: In vitro andin vivo evaluation. In: Pakistan Journal of Pharmaceutical Sciences. 2010 ; Vol. 23, No. 2. pp. 194-200.
@article{f867fc0c5de344c3b3f0a93007fc34b5,
title = "Erythrocytes as carrier for prednisolone: In vitro andin vivo evaluation",
abstract = "Resealed erythrocytes, as drug delivery system has tremendous potential to achieve site specificity and prolonged release of drug thereby enhancing therapeutic index and patient compliance. In the present investigation erythrocytes obtained from healthy volunteers were loaded with prednisolone using preswell dilution and dilution technique with two different cross-linking agents, glutaraldehyde and dimethylsulphoxide. Carrier erythrocytes, having acceptable loading parameters showed increased percentage drug content with the addition of cross-linking agents. In vitro drug release followed zero-order kinetics, haemoglobin content was found to be satisfactory and osmotic fragility study indicated that increased drug entrapment efficiency was found at 0.3{\%}w/v concentration of sodium chloride (hypotonic solution). In vivo tissue distribution studies were carried out for optimized formulation and order of distribution was found to be Liver>Lung>Kidney> Spleen. The developed drug delivery system is endowed with several exclusive advantages and hence holds potential for further research and clinical application.",
author = "G.V. Shavi and R.C. Doijad and P.B. Deshpande and F.V. Manvi and S.R. Meka and N. Udupa and R. Omprakash and K. Dhirendra",
note = "Cited By :15 Export Date: 10 November 2017 Correspondence Address: Shavi, G. V.; Department of Pharmaceutics, KLES's College of Pharmacy, Belgaum-10, Karnataka State, India; email: gopal_shavi@rediffmail.com Chemicals/CAS: dimethyl sulfoxide, 67-68-5; glutaraldehyde, 111-30-8, 37245-61-7; hemoglobin, 9008-02-0; prednisolone, 50-24-8; sodium chloride, 7647-14-5; Cross-Linking Reagents; Dimethyl Sulfoxide, 67-68-5; Drug Carriers; Glutaral, 111-30-8; Hemoglobins; Prednisolone, 50-24-8 Manufacturers: Wyeth Lederle, India References: Carstensen, S.T., (1998) Drug Stability, Principles and Practices, pp. 538-559. , 2nd Revised and Expanded edn., Marcel Dekker, New York; Dale, G.L., Villacorte, D.G., Beutler, E., (1997) High Yield Entrapment of Proteins into Erythrocytes, 18 (2), pp. 220-225; Dhir, S.S., Bhaskaran, S., Resealed erythrocytes as carriers for salbutamol sulphate (1995) Ind. J. Pharm. Sci., 57 (6), pp. 240-242; Eichler, H.G., Ramies, H., Bauer, K., Korn, A., Bacher, S., Gasic, S., Survival of Gentamicin loaded carrier erythrocytes in healthy human volunteers (1986) Eur. J. Clin. Invest., 16 (1), pp. 39-42; Gothoskar, A.V., Resealed erythrocytes: A review (2004) Pharm. Tech., pp. 1-6; Gutierrez Millan, C., Sayalero Marinero, M.L., Zarzuelo Castaneda, A., Lanao, J.M., Drug, enzyme and peptide delivery using erythrocytes as carriers (2004) J. Control Rel., 95, pp. 27-49; Tajerzadeh, H., Hamidi, M., Evaluation of hypotonic preswelling method for encapsulation of enalaprilat in intact human erythrocytes (2000) Drug Development and Industrial Pharmacy, 26 (12), pp. 1247-1257. , DOI 10.1081/DDC-100102306; Jain, S., Jain, N.K., Engineered erythrocytes as drug delivery system (1997) Ind. J. Pharm. Sci., 59 (6), pp. 275-281; Khar, R.K., Diwan, M., Targeted delivery of drugs (2001) Advances in Controlled and Novel Drug Delivery, pp. 420-456. , Jain NK (editor). CBS Publishers and Distributors, New Delhi; Lewis, D.A., Alpar, O.H., Therapeutic possibilities of drugs encapsulated in erythrocytes (1984) Int. J. Pharm., 22, pp. 137-146; Lynch, W.E., Sartino, G.P., Chaffar, A., Erythrocytes as carriers of chemotherapeutuic agents for targeting the reticuloendothelial system (1980) Am. J. Hematol., 9 (3), pp. 249-259; Magnani, M., Rossi, L., D'Ascenzo, M., Panzani, I., Bigi, L., Zanella, A., Erythrocyte engineering for drug delivery and targeting (1998) Biotechnology and Applied Biochemistry, 28 (1), pp. 1-6; Mishra, P.R., Jain, N.K., Biotin methroxate loaded erythrocytes for enhanced liver uptake. A study on rat (2002) Int. J. Pharm., 231 (2), pp. 145-153; Mishra, P.R., Jain, S., Jain, N.K., Engineered erythrocytes as a carrier for ciprofloxacin (1996) Drug Delivery, 3, pp. 239-244; Murthy, R.S.R., Soni, N.H., Sawant, K.K., Investigation on resealed erythrocytes as carriers for 5-flurouracil (2001) Ind. J. Pharm. Sci., 63 (2), pp. 105-109; Nicholas, B., Retrometabolic approaches to drug targeting (1995) Membrane and Barriers, pp. 1-6. , Rapaka RS (editor). NIH Publications; Noel-Hocquet, S., Jabbouri, S., Lazar, S., Maunier, J.C., Guillaumet, C., Ropars, C., Erythrocytes as carriers of new anti-opiod prodrugs: In vitro studies (1992) Advances in Experimental Medicine and Biology, 326. , The use of resealed erythrocytes as carriers and bioreactors. Magnani M and DeLoach JR (Eds.), Pslenum Press, New York; Ogiso, T., Iwaki, M., Othori, A., Encapsulation of dexamthasone in rabbit erythrocytes, the disposotion in circulation and anti-inflammatory effect (1985) J. Pharmacobiodyn, 8 (12), pp. 1032-1040; Pinilla, M., Jordan, J.A., Danz, J.C., Luque, L., In vitro stability properties of cross-linked red blood cells (1994) Adv. Biosci., 92, pp. 7-16; Richieri, G., Mel, H.C., Temperature effects on osmotic fragility, and the erythrocyte membrane (1985) Biochimica et Biophysica Acta - Biomembranes, 813 (1), pp. 41-50. , DOI 10.1016/0005-2736(85)90343-8; Rossi, L., Serafini, S., Cappellacci, L., Balestra, E., Brandi, G., Schiavano, G.F., Franchetti, P., Magnani, M., Erythrocyte-mediated delivery of a new homodinucleotide active against human immunodeficiency virus and herpes simplex virus (2001) Journal of Antimicrobial Chemotherapy, 47 (6), pp. 819-827; Sanz, S., Lizano, C., Luque, J., Pinilla, M., In vitro and in vivo study of glutumate dehydrogenase encapsulated into mouse erythrocytes by a hypotonic dialysis procedure (1999) Life Sci., 65 (26), pp. 2781-2789; Tajerzadeh, H., Hamidi, M., Dehpour, A., Rouini, M., Mehr, S., In vitro characterization of human inact erythrocytes loaded by Enalaprilat (2001) Drug Delivery, 8, pp. 223-230; Tesconi, S., Sepassi, K., (1999) J. Pharm. Sci., 88 (5), pp. 501-505; Vyas, S.P., Jaitely, V., Jain, S., Venkatesan, N., Kanaujia, P., Resealed erythrocytes: Drug carrier potentials and biomedical applications (1986) Indian Drugs, 33 (12), pp. 589-594; Vyas, S.P., Khar, R.K., (2002) Targeted and Controlled Drug Delivery - Novel Carrier Systems, pp. 387-415. , CBS Publishes and Distributors, New Delhi; Wadke, P.A., Jacobson, S., Preformulation testing specific delivery of mitomycin C to the liver, spleen and lung; nano and microspherical carriers of gelatin (1980) Int. J. Pharm., 81, pp. 131-141; Zolla, L., Lupidi, G., Marcheggiani, M., Falcioni, G., Brunori, M., Encapsulation of proteins into human erythrocytes: A kinetic investigation (1990) Biochimica et Biophysica Acta - Biomembranes, 1024 (1), pp. 5-9. , DOI 10.1016/0005-2736(90)90202-Y",
year = "2010",
month = "4",
doi = "10.1016/0005-2736(90)90202-Y",
language = "English",
volume = "23",
pages = "194--200",
journal = "Pakistan Journal of Pharmaceutical Sciences",
issn = "1011-601X",
publisher = "Pakistan Journal of Pharmaceutical Sciences",
number = "2",

}

Erythrocytes as carrier for prednisolone: In vitro andin vivo evaluation. / Shavi, G.V.; Doijad, R.C.; Deshpande, P.B.; Manvi, F.V.; Meka, S.R.; Udupa, N.; Omprakash, R.; Dhirendra, K.

In: Pakistan Journal of Pharmaceutical Sciences, Vol. 23, No. 2, 04.2010, p. 194-200.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Erythrocytes as carrier for prednisolone: In vitro andin vivo evaluation

AU - Shavi, G.V.

AU - Doijad, R.C.

AU - Deshpande, P.B.

AU - Manvi, F.V.

AU - Meka, S.R.

AU - Udupa, N.

AU - Omprakash, R.

AU - Dhirendra, K.

N1 - Cited By :15 Export Date: 10 November 2017 Correspondence Address: Shavi, G. V.; Department of Pharmaceutics, KLES's College of Pharmacy, Belgaum-10, Karnataka State, India; email: gopal_shavi@rediffmail.com Chemicals/CAS: dimethyl sulfoxide, 67-68-5; glutaraldehyde, 111-30-8, 37245-61-7; hemoglobin, 9008-02-0; prednisolone, 50-24-8; sodium chloride, 7647-14-5; Cross-Linking Reagents; Dimethyl Sulfoxide, 67-68-5; Drug Carriers; Glutaral, 111-30-8; Hemoglobins; Prednisolone, 50-24-8 Manufacturers: Wyeth Lederle, India References: Carstensen, S.T., (1998) Drug Stability, Principles and Practices, pp. 538-559. , 2nd Revised and Expanded edn., Marcel Dekker, New York; Dale, G.L., Villacorte, D.G., Beutler, E., (1997) High Yield Entrapment of Proteins into Erythrocytes, 18 (2), pp. 220-225; Dhir, S.S., Bhaskaran, S., Resealed erythrocytes as carriers for salbutamol sulphate (1995) Ind. J. Pharm. Sci., 57 (6), pp. 240-242; Eichler, H.G., Ramies, H., Bauer, K., Korn, A., Bacher, S., Gasic, S., Survival of Gentamicin loaded carrier erythrocytes in healthy human volunteers (1986) Eur. J. Clin. Invest., 16 (1), pp. 39-42; Gothoskar, A.V., Resealed erythrocytes: A review (2004) Pharm. Tech., pp. 1-6; Gutierrez Millan, C., Sayalero Marinero, M.L., Zarzuelo Castaneda, A., Lanao, J.M., Drug, enzyme and peptide delivery using erythrocytes as carriers (2004) J. Control Rel., 95, pp. 27-49; Tajerzadeh, H., Hamidi, M., Evaluation of hypotonic preswelling method for encapsulation of enalaprilat in intact human erythrocytes (2000) Drug Development and Industrial Pharmacy, 26 (12), pp. 1247-1257. , DOI 10.1081/DDC-100102306; Jain, S., Jain, N.K., Engineered erythrocytes as drug delivery system (1997) Ind. J. Pharm. Sci., 59 (6), pp. 275-281; Khar, R.K., Diwan, M., Targeted delivery of drugs (2001) Advances in Controlled and Novel Drug Delivery, pp. 420-456. , Jain NK (editor). CBS Publishers and Distributors, New Delhi; Lewis, D.A., Alpar, O.H., Therapeutic possibilities of drugs encapsulated in erythrocytes (1984) Int. J. Pharm., 22, pp. 137-146; Lynch, W.E., Sartino, G.P., Chaffar, A., Erythrocytes as carriers of chemotherapeutuic agents for targeting the reticuloendothelial system (1980) Am. J. Hematol., 9 (3), pp. 249-259; Magnani, M., Rossi, L., D'Ascenzo, M., Panzani, I., Bigi, L., Zanella, A., Erythrocyte engineering for drug delivery and targeting (1998) Biotechnology and Applied Biochemistry, 28 (1), pp. 1-6; Mishra, P.R., Jain, N.K., Biotin methroxate loaded erythrocytes for enhanced liver uptake. A study on rat (2002) Int. J. Pharm., 231 (2), pp. 145-153; Mishra, P.R., Jain, S., Jain, N.K., Engineered erythrocytes as a carrier for ciprofloxacin (1996) Drug Delivery, 3, pp. 239-244; Murthy, R.S.R., Soni, N.H., Sawant, K.K., Investigation on resealed erythrocytes as carriers for 5-flurouracil (2001) Ind. J. Pharm. Sci., 63 (2), pp. 105-109; Nicholas, B., Retrometabolic approaches to drug targeting (1995) Membrane and Barriers, pp. 1-6. , Rapaka RS (editor). NIH Publications; Noel-Hocquet, S., Jabbouri, S., Lazar, S., Maunier, J.C., Guillaumet, C., Ropars, C., Erythrocytes as carriers of new anti-opiod prodrugs: In vitro studies (1992) Advances in Experimental Medicine and Biology, 326. , The use of resealed erythrocytes as carriers and bioreactors. Magnani M and DeLoach JR (Eds.), Pslenum Press, New York; Ogiso, T., Iwaki, M., Othori, A., Encapsulation of dexamthasone in rabbit erythrocytes, the disposotion in circulation and anti-inflammatory effect (1985) J. Pharmacobiodyn, 8 (12), pp. 1032-1040; Pinilla, M., Jordan, J.A., Danz, J.C., Luque, L., In vitro stability properties of cross-linked red blood cells (1994) Adv. Biosci., 92, pp. 7-16; Richieri, G., Mel, H.C., Temperature effects on osmotic fragility, and the erythrocyte membrane (1985) Biochimica et Biophysica Acta - Biomembranes, 813 (1), pp. 41-50. , DOI 10.1016/0005-2736(85)90343-8; Rossi, L., Serafini, S., Cappellacci, L., Balestra, E., Brandi, G., Schiavano, G.F., Franchetti, P., Magnani, M., Erythrocyte-mediated delivery of a new homodinucleotide active against human immunodeficiency virus and herpes simplex virus (2001) Journal of Antimicrobial Chemotherapy, 47 (6), pp. 819-827; Sanz, S., Lizano, C., Luque, J., Pinilla, M., In vitro and in vivo study of glutumate dehydrogenase encapsulated into mouse erythrocytes by a hypotonic dialysis procedure (1999) Life Sci., 65 (26), pp. 2781-2789; Tajerzadeh, H., Hamidi, M., Dehpour, A., Rouini, M., Mehr, S., In vitro characterization of human inact erythrocytes loaded by Enalaprilat (2001) Drug Delivery, 8, pp. 223-230; Tesconi, S., Sepassi, K., (1999) J. Pharm. Sci., 88 (5), pp. 501-505; Vyas, S.P., Jaitely, V., Jain, S., Venkatesan, N., Kanaujia, P., Resealed erythrocytes: Drug carrier potentials and biomedical applications (1986) Indian Drugs, 33 (12), pp. 589-594; Vyas, S.P., Khar, R.K., (2002) Targeted and Controlled Drug Delivery - Novel Carrier Systems, pp. 387-415. , CBS Publishes and Distributors, New Delhi; Wadke, P.A., Jacobson, S., Preformulation testing specific delivery of mitomycin C to the liver, spleen and lung; nano and microspherical carriers of gelatin (1980) Int. J. Pharm., 81, pp. 131-141; Zolla, L., Lupidi, G., Marcheggiani, M., Falcioni, G., Brunori, M., Encapsulation of proteins into human erythrocytes: A kinetic investigation (1990) Biochimica et Biophysica Acta - Biomembranes, 1024 (1), pp. 5-9. , DOI 10.1016/0005-2736(90)90202-Y

PY - 2010/4

Y1 - 2010/4

N2 - Resealed erythrocytes, as drug delivery system has tremendous potential to achieve site specificity and prolonged release of drug thereby enhancing therapeutic index and patient compliance. In the present investigation erythrocytes obtained from healthy volunteers were loaded with prednisolone using preswell dilution and dilution technique with two different cross-linking agents, glutaraldehyde and dimethylsulphoxide. Carrier erythrocytes, having acceptable loading parameters showed increased percentage drug content with the addition of cross-linking agents. In vitro drug release followed zero-order kinetics, haemoglobin content was found to be satisfactory and osmotic fragility study indicated that increased drug entrapment efficiency was found at 0.3%w/v concentration of sodium chloride (hypotonic solution). In vivo tissue distribution studies were carried out for optimized formulation and order of distribution was found to be Liver>Lung>Kidney> Spleen. The developed drug delivery system is endowed with several exclusive advantages and hence holds potential for further research and clinical application.

AB - Resealed erythrocytes, as drug delivery system has tremendous potential to achieve site specificity and prolonged release of drug thereby enhancing therapeutic index and patient compliance. In the present investigation erythrocytes obtained from healthy volunteers were loaded with prednisolone using preswell dilution and dilution technique with two different cross-linking agents, glutaraldehyde and dimethylsulphoxide. Carrier erythrocytes, having acceptable loading parameters showed increased percentage drug content with the addition of cross-linking agents. In vitro drug release followed zero-order kinetics, haemoglobin content was found to be satisfactory and osmotic fragility study indicated that increased drug entrapment efficiency was found at 0.3%w/v concentration of sodium chloride (hypotonic solution). In vivo tissue distribution studies were carried out for optimized formulation and order of distribution was found to be Liver>Lung>Kidney> Spleen. The developed drug delivery system is endowed with several exclusive advantages and hence holds potential for further research and clinical application.

UR - http://www.scopus.com/inward/record.url?scp=77951744861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951744861&partnerID=8YFLogxK

U2 - 10.1016/0005-2736(90)90202-Y

DO - 10.1016/0005-2736(90)90202-Y

M3 - Article

VL - 23

SP - 194

EP - 200

JO - Pakistan Journal of Pharmaceutical Sciences

JF - Pakistan Journal of Pharmaceutical Sciences

SN - 1011-601X

IS - 2

ER -