Estimation of antimicrobial properties of aqueous and alcoholic extracts of Salvadora Persica (Miswak) on oral microbial pathogens - An invitro study

Salman Siddeeqh, Amrita Parida, Maji Jose, Vidya Pai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: Twigs of Salvadora persica (Miswak) plant are being used as a means of oral hygiene since ages for brushing teeth. Though clinical research and trials have shown promising results on effectiveness of Miswak, but some reports are conflicting. Aim: To evaluate the antimicrobial activity of crude aqueous and alcoholic extracts of Salvadora persica (Miswak) against the common microbial pathogens causing dental caries and periodontitis. Materials and Methods: A prospective study of one year duration was conducted in Yenopoya dental and medical college, Mangalore. The twigs of Salvadora persica were collected and alcoholic and aqueous extracts were prepared using standard techniques. The antimicrobial properties of the extracts against common oral pathogens like Streptococcus mutans, Streptococcus mitis, Candida albicans, Lactobacillus acidophilus, Prevotella intermedia, & Peptostreptococcus were performed by agar well diffusion method and two fold broth dilution method. Results: No significant results was obtained when water extracts of Salvadoraa persica was tested except for minimum inhibitory effect against Streptococcus mutans, Prevotella intermedia & Peptostreptococcus and Candida albicans. Relatively significant inhibitory effect was noted with respect to alcoholic extract of Salvadoraa persica. Conclusion: Although comparatively less than chlorhexidine which is a known antimicrobial agent, the alcoholic extracts of Salvadoraa persica showed antimicrobial effect against the common microbial pathogens causing dental caries and periodontitis indicating a potential beneficial effect of this plant. However, further research with more standardized extraction procedure and advanced techniques is required to find out the exact chemicals responsible for the antimicrobial properties of the plant extract.

Original languageEnglish
Pages (from-to)FC13-FC16
JournalJournal of Clinical and Diagnostic Research
Volume10
Issue number9
DOIs
Publication statusPublished - 01-09-2016

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Salvadoraceae
Pathogens
Candida
Prevotella intermedia
Peptostreptococcus
Streptococcus mutans
Periodontitis
Dental Caries
Candida albicans
Tooth
Chlorhexidine
Streptococcus mitis
Plant Extracts
Anti-Infective Agents
Lactobacillus acidophilus
Dilution
Agar
Oral Hygiene
Research
Water

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

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title = "Estimation of antimicrobial properties of aqueous and alcoholic extracts of Salvadora Persica (Miswak) on oral microbial pathogens - An invitro study",
abstract = "Introduction: Twigs of Salvadora persica (Miswak) plant are being used as a means of oral hygiene since ages for brushing teeth. Though clinical research and trials have shown promising results on effectiveness of Miswak, but some reports are conflicting. Aim: To evaluate the antimicrobial activity of crude aqueous and alcoholic extracts of Salvadora persica (Miswak) against the common microbial pathogens causing dental caries and periodontitis. Materials and Methods: A prospective study of one year duration was conducted in Yenopoya dental and medical college, Mangalore. The twigs of Salvadora persica were collected and alcoholic and aqueous extracts were prepared using standard techniques. The antimicrobial properties of the extracts against common oral pathogens like Streptococcus mutans, Streptococcus mitis, Candida albicans, Lactobacillus acidophilus, Prevotella intermedia, & Peptostreptococcus were performed by agar well diffusion method and two fold broth dilution method. Results: No significant results was obtained when water extracts of Salvadoraa persica was tested except for minimum inhibitory effect against Streptococcus mutans, Prevotella intermedia & Peptostreptococcus and Candida albicans. Relatively significant inhibitory effect was noted with respect to alcoholic extract of Salvadoraa persica. Conclusion: Although comparatively less than chlorhexidine which is a known antimicrobial agent, the alcoholic extracts of Salvadoraa persica showed antimicrobial effect against the common microbial pathogens causing dental caries and periodontitis indicating a potential beneficial effect of this plant. However, further research with more standardized extraction procedure and advanced techniques is required to find out the exact chemicals responsible for the antimicrobial properties of the plant extract.",
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Estimation of antimicrobial properties of aqueous and alcoholic extracts of Salvadora Persica (Miswak) on oral microbial pathogens - An invitro study. / Siddeeqh, Salman; Parida, Amrita; Jose, Maji; Pai, Vidya.

In: Journal of Clinical and Diagnostic Research, Vol. 10, No. 9, 01.09.2016, p. FC13-FC16.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Estimation of antimicrobial properties of aqueous and alcoholic extracts of Salvadora Persica (Miswak) on oral microbial pathogens - An invitro study

AU - Siddeeqh, Salman

AU - Parida, Amrita

AU - Jose, Maji

AU - Pai, Vidya

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N2 - Introduction: Twigs of Salvadora persica (Miswak) plant are being used as a means of oral hygiene since ages for brushing teeth. Though clinical research and trials have shown promising results on effectiveness of Miswak, but some reports are conflicting. Aim: To evaluate the antimicrobial activity of crude aqueous and alcoholic extracts of Salvadora persica (Miswak) against the common microbial pathogens causing dental caries and periodontitis. Materials and Methods: A prospective study of one year duration was conducted in Yenopoya dental and medical college, Mangalore. The twigs of Salvadora persica were collected and alcoholic and aqueous extracts were prepared using standard techniques. The antimicrobial properties of the extracts against common oral pathogens like Streptococcus mutans, Streptococcus mitis, Candida albicans, Lactobacillus acidophilus, Prevotella intermedia, & Peptostreptococcus were performed by agar well diffusion method and two fold broth dilution method. Results: No significant results was obtained when water extracts of Salvadoraa persica was tested except for minimum inhibitory effect against Streptococcus mutans, Prevotella intermedia & Peptostreptococcus and Candida albicans. Relatively significant inhibitory effect was noted with respect to alcoholic extract of Salvadoraa persica. Conclusion: Although comparatively less than chlorhexidine which is a known antimicrobial agent, the alcoholic extracts of Salvadoraa persica showed antimicrobial effect against the common microbial pathogens causing dental caries and periodontitis indicating a potential beneficial effect of this plant. However, further research with more standardized extraction procedure and advanced techniques is required to find out the exact chemicals responsible for the antimicrobial properties of the plant extract.

AB - Introduction: Twigs of Salvadora persica (Miswak) plant are being used as a means of oral hygiene since ages for brushing teeth. Though clinical research and trials have shown promising results on effectiveness of Miswak, but some reports are conflicting. Aim: To evaluate the antimicrobial activity of crude aqueous and alcoholic extracts of Salvadora persica (Miswak) against the common microbial pathogens causing dental caries and periodontitis. Materials and Methods: A prospective study of one year duration was conducted in Yenopoya dental and medical college, Mangalore. The twigs of Salvadora persica were collected and alcoholic and aqueous extracts were prepared using standard techniques. The antimicrobial properties of the extracts against common oral pathogens like Streptococcus mutans, Streptococcus mitis, Candida albicans, Lactobacillus acidophilus, Prevotella intermedia, & Peptostreptococcus were performed by agar well diffusion method and two fold broth dilution method. Results: No significant results was obtained when water extracts of Salvadoraa persica was tested except for minimum inhibitory effect against Streptococcus mutans, Prevotella intermedia & Peptostreptococcus and Candida albicans. Relatively significant inhibitory effect was noted with respect to alcoholic extract of Salvadoraa persica. Conclusion: Although comparatively less than chlorhexidine which is a known antimicrobial agent, the alcoholic extracts of Salvadoraa persica showed antimicrobial effect against the common microbial pathogens causing dental caries and periodontitis indicating a potential beneficial effect of this plant. However, further research with more standardized extraction procedure and advanced techniques is required to find out the exact chemicals responsible for the antimicrobial properties of the plant extract.

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