Estimation of esomeprazole in human plasma by reverse phase high performance liquid chromatography

R. Shetty, G. Subramanian, A. Ranjith Kumar, S. Pandey, N. Udupa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A simple sensitive and specific high performance liquid chromatography (HPLC) method was developed for the determination of esomeprazole in capsules and human plasma. Lansoprazole was used as an internal standard. Separation of Esomeprazole and internal standard was carried out on reverse phase C 18 column (250 x 4.6 mm, 5μ) using mobile phase acetonitrile and 20mM phosphate buffer pH 3.2 (30:70v/v) and UV visible detection at 300 nm without interference from endogenous materials. The method involves simple extraction procedure prior to chromatographic analysis. The method showed good linearity, accuracy and precision in both the lower and higher ranges of calibration in capsules and plasma. The limit of detection and quantitation of Esomeprazole in plasma was 10 ng/ml and 20 ng/ml respectively.

Original languageEnglish
Pages (from-to)158-161
Number of pages4
JournalIndian Drugs
Volume42
Issue number3
Publication statusPublished - 03-2005

Fingerprint

Esomeprazole
Reverse-Phase Chromatography
High Pressure Liquid Chromatography
Capsules
Lansoprazole
Calibration
Limit of Detection
Chromatography
Buffers
Phosphates

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Pharmaceutical Science
  • Pharmacology

Cite this

Shetty, R., Subramanian, G., Ranjith Kumar, A., Pandey, S., & Udupa, N. (2005). Estimation of esomeprazole in human plasma by reverse phase high performance liquid chromatography. Indian Drugs, 42(3), 158-161.
Shetty, R. ; Subramanian, G. ; Ranjith Kumar, A. ; Pandey, S. ; Udupa, N. / Estimation of esomeprazole in human plasma by reverse phase high performance liquid chromatography. In: Indian Drugs. 2005 ; Vol. 42, No. 3. pp. 158-161.
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Shetty, R, Subramanian, G, Ranjith Kumar, A, Pandey, S & Udupa, N 2005, 'Estimation of esomeprazole in human plasma by reverse phase high performance liquid chromatography', Indian Drugs, vol. 42, no. 3, pp. 158-161.

Estimation of esomeprazole in human plasma by reverse phase high performance liquid chromatography. / Shetty, R.; Subramanian, G.; Ranjith Kumar, A.; Pandey, S.; Udupa, N.

In: Indian Drugs, Vol. 42, No. 3, 03.2005, p. 158-161.

Research output: Contribution to journalArticle

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T1 - Estimation of esomeprazole in human plasma by reverse phase high performance liquid chromatography

AU - Shetty, R.

AU - Subramanian, G.

AU - Ranjith Kumar, A.

AU - Pandey, S.

AU - Udupa, N.

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AB - A simple sensitive and specific high performance liquid chromatography (HPLC) method was developed for the determination of esomeprazole in capsules and human plasma. Lansoprazole was used as an internal standard. Separation of Esomeprazole and internal standard was carried out on reverse phase C 18 column (250 x 4.6 mm, 5μ) using mobile phase acetonitrile and 20mM phosphate buffer pH 3.2 (30:70v/v) and UV visible detection at 300 nm without interference from endogenous materials. The method involves simple extraction procedure prior to chromatographic analysis. The method showed good linearity, accuracy and precision in both the lower and higher ranges of calibration in capsules and plasma. The limit of detection and quantitation of Esomeprazole in plasma was 10 ng/ml and 20 ng/ml respectively.

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