Abstract

A simple, rapid and sensitive high performance liquid chromatographic method was developed for the analysis of nicotine in plasma. This method involved simple extraction procedure prior to chromatographic analysis. The chromatographic separation was achieved on a reverse phase C18 column with ultraviolet detection at 260 nm. The mobile phase consisted of 0.3% triethylamine buffer adjusted to pH 7.0 using phosphoric acid and methanol in the ratio of 60:40. The isocratic system was operated at ambient temperature and requires 20 min of chromatographic time at a flow rate of 1 mL/min. Acetanilide was used as an internal standard. The recovery was found to be 85.3% with interday precision of about 6.60 %. The extraction method of nicotine from plasma was simple, rapid, sensitive and no interference from plasma components observed.

Original languageEnglish
Pages (from-to)28-31
Number of pages4
JournalIndian Drugs
Volume41
Issue number1
Publication statusPublished - 01-2004

Fingerprint

Nicotine
High Pressure Liquid Chromatography
Methanol
Chromatography
Buffers
Temperature

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

Cite this

Lewis, S. ; Subramanian, G. ; Udupa, N. / Estimation of nicotine in human plasma by HPLC. In: Indian Drugs. 2004 ; Vol. 41, No. 1. pp. 28-31.
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Lewis, S, Subramanian, G & Udupa, N 2004, 'Estimation of nicotine in human plasma by HPLC', Indian Drugs, vol. 41, no. 1, pp. 28-31.

Estimation of nicotine in human plasma by HPLC. / Lewis, S.; Subramanian, G.; Udupa, N.

In: Indian Drugs, Vol. 41, No. 1, 01.2004, p. 28-31.

Research output: Contribution to journalArticle

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T1 - Estimation of nicotine in human plasma by HPLC

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AU - Subramanian, G.

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