Estimation of stavudine, lamivudine and nevirapine by chemometric UV spectroscopic method

Abhilasha Gupta, S. T. Bhagawati, B. S. Muddukrishna, P. C. Jagadish, Krishnamurthy Bhat

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2 Citations (Scopus)

Abstract

A chemometric UV-spectrophotometric method of analysis was successfully developed for the simultaneous determination of stavudine, lamivudine and nevirapine in pharmaceutical formulation. The application of multivariate method to the obtained spectrophotometric data is a new idea for the simultaneous quantitative analysis of stavudine, lamivudine and nevirapine in samples. Multivariate calibration models were built from the raw PLS(Partial least square) method. Verification of the calibration carried out with the aid of prediction set of mixtures of these three drugs produced satisfactory resuit. The results also showed that for practical purposes, PLS model constructed was effective in their performance prediction. The precision study showed that % RSD was within the range of acceptable limits, the mean recovery (mean ± SD) was found to be 100.24 ± 1.23,100.52 ± 1.52, 99.72 ± 1.57 and 100.23 ± 1.55, 99.66 ± 1.03, 99.15 ±1.39 for stavudine, lamivudine and nevirapine for two different batches of the formulation respectively. The good recoveries obtained in this case proved that, the proposed procedures could be applied efficiently for determination of studied drug simultaneously in their mixture as well as in the commercial dosage form with satisfactory precision and accuracy.

Original languageEnglish
Pages (from-to)197-205
Number of pages9
JournalIndian Journal of Pharmaceutical Education and Research
Volume46
Issue number3
Publication statusPublished - 07-2012

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Least-Squares Analysis
Calibration
Drug Compounding
Dosage Forms
Pharmaceutical Preparations
stavudine, lamivudine, nevirapine drug combination

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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title = "Estimation of stavudine, lamivudine and nevirapine by chemometric UV spectroscopic method",
abstract = "A chemometric UV-spectrophotometric method of analysis was successfully developed for the simultaneous determination of stavudine, lamivudine and nevirapine in pharmaceutical formulation. The application of multivariate method to the obtained spectrophotometric data is a new idea for the simultaneous quantitative analysis of stavudine, lamivudine and nevirapine in samples. Multivariate calibration models were built from the raw PLS(Partial least square) method. Verification of the calibration carried out with the aid of prediction set of mixtures of these three drugs produced satisfactory resuit. The results also showed that for practical purposes, PLS model constructed was effective in their performance prediction. The precision study showed that {\%} RSD was within the range of acceptable limits, the mean recovery (mean ± SD) was found to be 100.24 ± 1.23,100.52 ± 1.52, 99.72 ± 1.57 and 100.23 ± 1.55, 99.66 ± 1.03, 99.15 ±1.39 for stavudine, lamivudine and nevirapine for two different batches of the formulation respectively. The good recoveries obtained in this case proved that, the proposed procedures could be applied efficiently for determination of studied drug simultaneously in their mixture as well as in the commercial dosage form with satisfactory precision and accuracy.",
author = "Abhilasha Gupta and Bhagawati, {S. T.} and Muddukrishna, {B. S.} and Jagadish, {P. C.} and Krishnamurthy Bhat",
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AU - Gupta, Abhilasha

AU - Bhagawati, S. T.

AU - Muddukrishna, B. S.

AU - Jagadish, P. C.

AU - Bhat, Krishnamurthy

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AB - A chemometric UV-spectrophotometric method of analysis was successfully developed for the simultaneous determination of stavudine, lamivudine and nevirapine in pharmaceutical formulation. The application of multivariate method to the obtained spectrophotometric data is a new idea for the simultaneous quantitative analysis of stavudine, lamivudine and nevirapine in samples. Multivariate calibration models were built from the raw PLS(Partial least square) method. Verification of the calibration carried out with the aid of prediction set of mixtures of these three drugs produced satisfactory resuit. The results also showed that for practical purposes, PLS model constructed was effective in their performance prediction. The precision study showed that % RSD was within the range of acceptable limits, the mean recovery (mean ± SD) was found to be 100.24 ± 1.23,100.52 ± 1.52, 99.72 ± 1.57 and 100.23 ± 1.55, 99.66 ± 1.03, 99.15 ±1.39 for stavudine, lamivudine and nevirapine for two different batches of the formulation respectively. The good recoveries obtained in this case proved that, the proposed procedures could be applied efficiently for determination of studied drug simultaneously in their mixture as well as in the commercial dosage form with satisfactory precision and accuracy.

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