Estimation of the fibre-composition of verhoff-stained fascia using colour-image processing

Rohit Nayak, A. K. Pandey, Pramod Kumar, Ramesh R. Galigekere

Research output: Contribution to journalArticle

Abstract

This paper reports the results of our work on assessing the composition of abdominal fascia based on microscopic images of specimen stained by Verhoff's method, using colour-image processing. Verhoff'1s method of staining results in collagen fibres appearing red, and elastic fibres and speckle-like artefacts appearing black (elastic fibres being darker), in a yellowish background. The basic idea involves segmenting the red-coloured collagen first, followed by segmenting the black elastic fibres. We use the YIQ colour model for the purpose. Collagen is extracted by thresholding a contrast-enhanced version of the I-image, and elastic fibres are extracted by thresholding the Y-image based on an empirically estimated threshold-value. Results of experiments on real microscopic images are encouraging.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalInternational Journal of Medical Engineering and Informatics
Volume3
Issue number2
DOIs
Publication statusPublished - 07-2011

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Color image processing
Elastic Tissue
Fascia
Color
Collagen
Fibers
Chemical analysis
Artifacts
Speckle
Staining and Labeling
Experiments

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Health Informatics

Cite this

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abstract = "This paper reports the results of our work on assessing the composition of abdominal fascia based on microscopic images of specimen stained by Verhoff's method, using colour-image processing. Verhoff'1s method of staining results in collagen fibres appearing red, and elastic fibres and speckle-like artefacts appearing black (elastic fibres being darker), in a yellowish background. The basic idea involves segmenting the red-coloured collagen first, followed by segmenting the black elastic fibres. We use the YIQ colour model for the purpose. Collagen is extracted by thresholding a contrast-enhanced version of the I-image, and elastic fibres are extracted by thresholding the Y-image based on an empirically estimated threshold-value. Results of experiments on real microscopic images are encouraging.",
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Estimation of the fibre-composition of verhoff-stained fascia using colour-image processing. / Nayak, Rohit; Pandey, A. K.; Kumar, Pramod; Galigekere, Ramesh R.

In: International Journal of Medical Engineering and Informatics, Vol. 3, No. 2, 07.2011, p. 99-107.

Research output: Contribution to journalArticle

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