Estimation of total phenols with special emphasis to antioxidant potentials of few hypericum species

H. Raghu Chandrashekhar, P. Venkatesh, M. Arumugam, P. Vijayan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In present investigation, the phenolic content and antioxidant activity of leaf extracts (methanolic) of Hypericum mysorense, H. perforatum, H. japonicum and H. patulum were studied. Various in vitro antioxidant models (ABTS, DPPH, H 2O 2, Nitric oxide, LPO, Alkaline DMSO, pNDA) were carried out for all methanolic extracts of hypericum species. The total phenolic and flavanol content were performed in order to correlate whether the antioxidant activity of extracts is influenced by the concentration of flavonols and/or phenols present. The higher percentage of total phenolic and flavonol content was found to be 24.72 and 101.7 mg/g in H. mysorense and H japonicum extract respectively. The H. mysorense exhibited stronger free radical scavenging activity as evidenced by the low IC 50 values in DPPH (3.35 μg/ml), ABTS (29.5 μg/ ml), H 2O 2 (58 μg/ml), and Lipid peroxidation (13 μg/ml) methods, and H. perforatum exhibited its potent activity against ABTS (28.5 μg/ml), DPPH (7.5 μg/ml), and H. japonicum exhibited its H 2O 2 scavenging activity at 49 μg/ml. The significance of the obtained values are distinguished when compared with the standards (Rutin) used. The results strongly suggest the exploration of effective lead from hypericum species will be alternative choice for the treatment of pathologies caused in the consequences of excess free radical production.
Original languageEnglish
Pages (from-to)680-687
Number of pages8
JournalPharmacologyonline
Volume1
Publication statusPublished - 2009

Fingerprint

Hypericum
Phenols
Antioxidants
Free Radicals
Rutin
Flavonols
Dimethyl Sulfoxide
Lipid Peroxidation
Nitric Oxide
Pathology
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid

Cite this

@article{38629c342a9048d3be2eb43658d34fb4,
title = "Estimation of total phenols with special emphasis to antioxidant potentials of few hypericum species",
abstract = "In present investigation, the phenolic content and antioxidant activity of leaf extracts (methanolic) of Hypericum mysorense, H. perforatum, H. japonicum and H. patulum were studied. Various in vitro antioxidant models (ABTS, DPPH, H 2O 2, Nitric oxide, LPO, Alkaline DMSO, pNDA) were carried out for all methanolic extracts of hypericum species. The total phenolic and flavanol content were performed in order to correlate whether the antioxidant activity of extracts is influenced by the concentration of flavonols and/or phenols present. The higher percentage of total phenolic and flavonol content was found to be 24.72 and 101.7 mg/g in H. mysorense and H japonicum extract respectively. The H. mysorense exhibited stronger free radical scavenging activity as evidenced by the low IC 50 values in DPPH (3.35 μg/ml), ABTS (29.5 μg/ ml), H 2O 2 (58 μg/ml), and Lipid peroxidation (13 μg/ml) methods, and H. perforatum exhibited its potent activity against ABTS (28.5 μg/ml), DPPH (7.5 μg/ml), and H. japonicum exhibited its H 2O 2 scavenging activity at 49 μg/ml. The significance of the obtained values are distinguished when compared with the standards (Rutin) used. The results strongly suggest the exploration of effective lead from hypericum species will be alternative choice for the treatment of pathologies caused in the consequences of excess free radical production.",
author = "{Raghu Chandrashekhar}, H. and P. Venkatesh and M. Arumugam and P. Vijayan",
note = "Cited By :3 Export Date: 10 November 2017 Correspondence Address: Venkatesh, P.; Department of Pharmaceutical Biotechnology, J.S.S. College of Pharmacy, Ooty 643001 Tamil Nadu, India; email: venkybiotech@gmail.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), 28752-68-3; dimethyl sulfoxide, 67-68-5; flavonol, 577-85-5; hydrogen peroxide, 7722-84-1; methanol, 67-56-1; nitric oxide, 10102-43-9; rutoside, 153-18-4, 22519-99-9 References: Gu, M., Owen, A.D., Toffa, S.E., Cooper, J.M., Dexter, D.T., Jenner, P., Marsden, C.D., Schapira, A.H., Mitochondrial function, GSH and iron in neurodegeneration and Lewy body diseases (1998) J Neurol Sci, 158, pp. 24-29; Halliwell, B., Aeschbach, R., Loliger, J., Aruoma, O.I., The characterization of antioxidants (1995) Food Chem Toxicol, 33, pp. 601-617; Tseng, T.H., Kao, E.S., Chu, C.Y., Chou, F.P., Lin Wu, H.W., Wang, C.J., Protective effects of dried flower extracts of Hibiscus sabdariffia L. against oxidative stress in rat primary hepatocytes (1997) Food Chem Toxicol, 35, pp. 1159-1164; Madsen, H.L., Bertelsen, G., Spices as antioxidants (1995) Trends Food Sci Tech, 6, pp. 271-277; Rice-Evans, C.A., Sampson, J., Bramley, P.M., Hollowa, D.E., Why do we expect carotenoids to be antioxidants in vivo? (1997) Free Rad Res, 26, pp. 381-398; Newall, C.A., Anderson, L.A., Philipson, J.D., (1996) Herbal medicines, A Guide for Health-Care professionals, p. 250. , London, The pharmaceutical press;; Decosterd, L.A., Hoffmann, E., Kyburz, R., Bray, D., Hostettmann, K., A New Phloroglucinol Derivative from Hypericum calycinum with Antifungal and in vitro Antimalarial Activity (1991) Planta med, 7, pp. 548-551; Tada, M., Chiba, K., Yamada, H., Maruyama, H., Phloroglucinol derivatives as competitive inhibitors against thromboxane A 2 and leukotriene D 4 from Hypericum erectum (1991) Phytochem, 30, pp. 2559-2562; Chung, M.I., Lai, M.H., Yen, M.H., Wu, R.R., Lin, C.N., Phenolics from Hypericum geminiflorum (1997) Phytochem, 44, pp. 943-947; Wu, Q.L., Wang, S.P., Du, L.J., Zhang, S.M., Yang, J.S., Xiao, P.G., Chromone glycosides and flavonoids from Hypericum japonicum (1998) Phytochem, 49, pp. 1417-1420; Wu, Q.L., Wang, S.P., Du, L.J., Yang, J.S., Xiao, P.G., Xanthones from Hypericum japonicum and H. henryi (1998) Phytochem, 49, pp. 1395-1402; Decosterd, L.A., Stoeckli-Evans, H., Chapuis, J.C., Msonthi, J.D., Sordat, B., Hostettmann, K., New Hyperforin Derivatives from Hypericum revolutum VAHL with Growth-Inhibitory Activity against a Human Colon Carcinoma Cell Line (1989) Helv Chim Acta, 72, pp. 464-471; Brondz, I., Greibrokk, J., Aasen, A.J., n-Alkanes of hypericum perforatum: A revision (1983) Phytochem, 2, pp. 295-296; Kitanov, G.M., Hypericin and pseudohypericin in some Hypericum species (2001) Biochem Syst Ecol, 29, pp. 171-178; Cakir A, Duru ME, Harmandar M, Ciriminna R, Passannanti S, Piozzi F. Comparison of the volatile oils of Hypericum scabrum L. and Hypericum perforatum L. from Turkey. Flavour Frag J. 1997; 12: 285-287; Vijayan P, Vinodkumar S, Badami S, Mukherjee PK, Dhanaraj SA, Suresh B. Selective in vitro cytotoxicity of Hypericum hookerianum towards cancer cell lines. Oriental Pharm Expt Med. 2003; 3:141-146; Yamasaki, K., Hashimoto, A., Kokusenya, Y., Miyamoto, T., Sato, T., Electrochemical method for estimating the antioxidative effects of methanol extracts of crude drugs (1994) Chem Pharm Bull, 42, pp. 1663-1665; Garrat, D.C., (1964) The Quantitative Analysis of Drugs, 3, p. 456. , Japan: Chapman and Hall; Ruch, R., Cheng, S.J., Klaunig, J.E., Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea (1989) Carcinogenesis, 10, pp. 1003-1008; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice Evans, C., Antioxidant activity applying an improved ABTS radical cation decolorization assay (1999) Free rad Biol Med, 26, pp. 1231-1237; Kunchandy, E., Rao, M.N.A., Effect of curcumin on hydroxyl radical generation through Fenton reaction (1989) Int J Pharm, 57, pp. 173-176; Kunchandy, E., Rao, M.N.A., Oxygen radical scavenging activity of curcumin (1990) Int J Pharm, 58, pp. 237-240; Cook, N.C., Samman, S., Flavonoids-Chemistry, metabolism, cardioprotective effects, and dietary sources (1996) Nutr Biochem, 7, pp. 66-76; Sadasivam, S., Manickam, A., (1992) Biochemical methods for agricultural sciences, p. 187. , New Delhi: Wiley Eastern Ltd; Woisky, R., Salatino, A., Salatino, Analysis of propolis: Some parameters and procedures for chemical quality control (1998) J Agric Res, 37, pp. 99-105; Soares, J.R., Dins, T.C.P., Cunha, A.P., Almeida, L.M., Antioxidant activities of some extracts of Thymus zygis (1997) Free Rad Res, 26, pp. 469-478; Pellegrini, N., Simonetti, P., Gardana, C., Brenna, O., Brighenti, F., Pietta, P., Polyphenol Content and Total antioxidant activity of Vini Novelli (Young Red Wines) (2000) J Agric Food Chem, 48, pp. 732-735; Brown, J.E., Rice-Evans, C.A., Luteolin-rich artichoke extract protects low density lipoprotein from oxidation In vitro (1998) Free Rad Res, 29, pp. 247-255; Halliwell, B., Free radicals, antioxidants and human disease: Curiosity, cause or consequence (1994) Lancet, 344, pp. 721-724; Sun, T., Ho, C.T., Antioxidant activities of buckwheat extracts (2005) Food Chem, 90, pp. 743-749",
year = "2009",
language = "English",
volume = "1",
pages = "680--687",
journal = "Pharmacologyonline",
issn = "1827-8620",
publisher = "SILAE (Italo-Latin American Society of Ethnomedicine)",

}

Estimation of total phenols with special emphasis to antioxidant potentials of few hypericum species. / Raghu Chandrashekhar, H.; Venkatesh, P.; Arumugam, M.; Vijayan, P.

In: Pharmacologyonline, Vol. 1, 2009, p. 680-687.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Estimation of total phenols with special emphasis to antioxidant potentials of few hypericum species

AU - Raghu Chandrashekhar, H.

AU - Venkatesh, P.

AU - Arumugam, M.

AU - Vijayan, P.

N1 - Cited By :3 Export Date: 10 November 2017 Correspondence Address: Venkatesh, P.; Department of Pharmaceutical Biotechnology, J.S.S. College of Pharmacy, Ooty 643001 Tamil Nadu, India; email: venkybiotech@gmail.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), 28752-68-3; dimethyl sulfoxide, 67-68-5; flavonol, 577-85-5; hydrogen peroxide, 7722-84-1; methanol, 67-56-1; nitric oxide, 10102-43-9; rutoside, 153-18-4, 22519-99-9 References: Gu, M., Owen, A.D., Toffa, S.E., Cooper, J.M., Dexter, D.T., Jenner, P., Marsden, C.D., Schapira, A.H., Mitochondrial function, GSH and iron in neurodegeneration and Lewy body diseases (1998) J Neurol Sci, 158, pp. 24-29; Halliwell, B., Aeschbach, R., Loliger, J., Aruoma, O.I., The characterization of antioxidants (1995) Food Chem Toxicol, 33, pp. 601-617; Tseng, T.H., Kao, E.S., Chu, C.Y., Chou, F.P., Lin Wu, H.W., Wang, C.J., Protective effects of dried flower extracts of Hibiscus sabdariffia L. against oxidative stress in rat primary hepatocytes (1997) Food Chem Toxicol, 35, pp. 1159-1164; Madsen, H.L., Bertelsen, G., Spices as antioxidants (1995) Trends Food Sci Tech, 6, pp. 271-277; Rice-Evans, C.A., Sampson, J., Bramley, P.M., Hollowa, D.E., Why do we expect carotenoids to be antioxidants in vivo? (1997) Free Rad Res, 26, pp. 381-398; Newall, C.A., Anderson, L.A., Philipson, J.D., (1996) Herbal medicines, A Guide for Health-Care professionals, p. 250. , London, The pharmaceutical press;; Decosterd, L.A., Hoffmann, E., Kyburz, R., Bray, D., Hostettmann, K., A New Phloroglucinol Derivative from Hypericum calycinum with Antifungal and in vitro Antimalarial Activity (1991) Planta med, 7, pp. 548-551; Tada, M., Chiba, K., Yamada, H., Maruyama, H., Phloroglucinol derivatives as competitive inhibitors against thromboxane A 2 and leukotriene D 4 from Hypericum erectum (1991) Phytochem, 30, pp. 2559-2562; Chung, M.I., Lai, M.H., Yen, M.H., Wu, R.R., Lin, C.N., Phenolics from Hypericum geminiflorum (1997) Phytochem, 44, pp. 943-947; Wu, Q.L., Wang, S.P., Du, L.J., Zhang, S.M., Yang, J.S., Xiao, P.G., Chromone glycosides and flavonoids from Hypericum japonicum (1998) Phytochem, 49, pp. 1417-1420; Wu, Q.L., Wang, S.P., Du, L.J., Yang, J.S., Xiao, P.G., Xanthones from Hypericum japonicum and H. henryi (1998) Phytochem, 49, pp. 1395-1402; Decosterd, L.A., Stoeckli-Evans, H., Chapuis, J.C., Msonthi, J.D., Sordat, B., Hostettmann, K., New Hyperforin Derivatives from Hypericum revolutum VAHL with Growth-Inhibitory Activity against a Human Colon Carcinoma Cell Line (1989) Helv Chim Acta, 72, pp. 464-471; Brondz, I., Greibrokk, J., Aasen, A.J., n-Alkanes of hypericum perforatum: A revision (1983) Phytochem, 2, pp. 295-296; Kitanov, G.M., Hypericin and pseudohypericin in some Hypericum species (2001) Biochem Syst Ecol, 29, pp. 171-178; Cakir A, Duru ME, Harmandar M, Ciriminna R, Passannanti S, Piozzi F. Comparison of the volatile oils of Hypericum scabrum L. and Hypericum perforatum L. from Turkey. Flavour Frag J. 1997; 12: 285-287; Vijayan P, Vinodkumar S, Badami S, Mukherjee PK, Dhanaraj SA, Suresh B. Selective in vitro cytotoxicity of Hypericum hookerianum towards cancer cell lines. Oriental Pharm Expt Med. 2003; 3:141-146; Yamasaki, K., Hashimoto, A., Kokusenya, Y., Miyamoto, T., Sato, T., Electrochemical method for estimating the antioxidative effects of methanol extracts of crude drugs (1994) Chem Pharm Bull, 42, pp. 1663-1665; Garrat, D.C., (1964) The Quantitative Analysis of Drugs, 3, p. 456. , Japan: Chapman and Hall; Ruch, R., Cheng, S.J., Klaunig, J.E., Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea (1989) Carcinogenesis, 10, pp. 1003-1008; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice Evans, C., Antioxidant activity applying an improved ABTS radical cation decolorization assay (1999) Free rad Biol Med, 26, pp. 1231-1237; Kunchandy, E., Rao, M.N.A., Effect of curcumin on hydroxyl radical generation through Fenton reaction (1989) Int J Pharm, 57, pp. 173-176; Kunchandy, E., Rao, M.N.A., Oxygen radical scavenging activity of curcumin (1990) Int J Pharm, 58, pp. 237-240; Cook, N.C., Samman, S., Flavonoids-Chemistry, metabolism, cardioprotective effects, and dietary sources (1996) Nutr Biochem, 7, pp. 66-76; Sadasivam, S., Manickam, A., (1992) Biochemical methods for agricultural sciences, p. 187. , New Delhi: Wiley Eastern Ltd; Woisky, R., Salatino, A., Salatino, Analysis of propolis: Some parameters and procedures for chemical quality control (1998) J Agric Res, 37, pp. 99-105; Soares, J.R., Dins, T.C.P., Cunha, A.P., Almeida, L.M., Antioxidant activities of some extracts of Thymus zygis (1997) Free Rad Res, 26, pp. 469-478; Pellegrini, N., Simonetti, P., Gardana, C., Brenna, O., Brighenti, F., Pietta, P., Polyphenol Content and Total antioxidant activity of Vini Novelli (Young Red Wines) (2000) J Agric Food Chem, 48, pp. 732-735; Brown, J.E., Rice-Evans, C.A., Luteolin-rich artichoke extract protects low density lipoprotein from oxidation In vitro (1998) Free Rad Res, 29, pp. 247-255; Halliwell, B., Free radicals, antioxidants and human disease: Curiosity, cause or consequence (1994) Lancet, 344, pp. 721-724; Sun, T., Ho, C.T., Antioxidant activities of buckwheat extracts (2005) Food Chem, 90, pp. 743-749

PY - 2009

Y1 - 2009

N2 - In present investigation, the phenolic content and antioxidant activity of leaf extracts (methanolic) of Hypericum mysorense, H. perforatum, H. japonicum and H. patulum were studied. Various in vitro antioxidant models (ABTS, DPPH, H 2O 2, Nitric oxide, LPO, Alkaline DMSO, pNDA) were carried out for all methanolic extracts of hypericum species. The total phenolic and flavanol content were performed in order to correlate whether the antioxidant activity of extracts is influenced by the concentration of flavonols and/or phenols present. The higher percentage of total phenolic and flavonol content was found to be 24.72 and 101.7 mg/g in H. mysorense and H japonicum extract respectively. The H. mysorense exhibited stronger free radical scavenging activity as evidenced by the low IC 50 values in DPPH (3.35 μg/ml), ABTS (29.5 μg/ ml), H 2O 2 (58 μg/ml), and Lipid peroxidation (13 μg/ml) methods, and H. perforatum exhibited its potent activity against ABTS (28.5 μg/ml), DPPH (7.5 μg/ml), and H. japonicum exhibited its H 2O 2 scavenging activity at 49 μg/ml. The significance of the obtained values are distinguished when compared with the standards (Rutin) used. The results strongly suggest the exploration of effective lead from hypericum species will be alternative choice for the treatment of pathologies caused in the consequences of excess free radical production.

AB - In present investigation, the phenolic content and antioxidant activity of leaf extracts (methanolic) of Hypericum mysorense, H. perforatum, H. japonicum and H. patulum were studied. Various in vitro antioxidant models (ABTS, DPPH, H 2O 2, Nitric oxide, LPO, Alkaline DMSO, pNDA) were carried out for all methanolic extracts of hypericum species. The total phenolic and flavanol content were performed in order to correlate whether the antioxidant activity of extracts is influenced by the concentration of flavonols and/or phenols present. The higher percentage of total phenolic and flavonol content was found to be 24.72 and 101.7 mg/g in H. mysorense and H japonicum extract respectively. The H. mysorense exhibited stronger free radical scavenging activity as evidenced by the low IC 50 values in DPPH (3.35 μg/ml), ABTS (29.5 μg/ ml), H 2O 2 (58 μg/ml), and Lipid peroxidation (13 μg/ml) methods, and H. perforatum exhibited its potent activity against ABTS (28.5 μg/ml), DPPH (7.5 μg/ml), and H. japonicum exhibited its H 2O 2 scavenging activity at 49 μg/ml. The significance of the obtained values are distinguished when compared with the standards (Rutin) used. The results strongly suggest the exploration of effective lead from hypericum species will be alternative choice for the treatment of pathologies caused in the consequences of excess free radical production.

M3 - Article

VL - 1

SP - 680

EP - 687

JO - Pharmacologyonline

JF - Pharmacologyonline

SN - 1827-8620

ER -