Abstract

The present study reports the effect of ethambutol (EMB) on testicular function. Prepubertal and adult male Swiss albino mice were treated with 40, 80, 160 mg/kg body weight of EMB, intraperitoneally, every alternate day for 4 weeks. After 2 weeks gap, mice were sacrificed to collect caudal spermatozoa. EMB treatment resulted in a dose-dependent decrease in the testicular weight, sperm count and motility while the percentage of sperm with head abnormalities, immature chromatin (P < 0.001) and DNA damage increased (P < 0.01). In addition, EMB treatment resulted in significant depletion of glutathione (P < 0.05–P < 0.01) and histopathological abnormalities such as large cells, vacuolation of tubules and isolated colonies of spermatogenic cells were observed. Oct4, 17β-Hsd and c-Kit mRNA was marginally elevated in EMB treated testes at the highest dose studied. In conclusion, the result of the present study indicates that EMB has adverse effect on testicular function and impairs the sperm functional competence.

Original languageEnglish
Pages (from-to)28-37
Number of pages10
JournalEnvironmental Toxicology and Pharmacology
Volume47
DOIs
Publication statusPublished - 01-10-2016

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Ethambutol
Mental Competency
Spermatozoa
Sperm Head
Sperm Count
Sperm Motility
DNA Damage
Chromatin
Glutathione
Testis
Body Weight
Weights and Measures
Messenger RNA
DNA

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

Cite this

@article{b6a05bb929af47d8967b1b92ae1c634b,
title = "Ethambutol induces testicular damage and decreases the sperm functional competence in Swiss albino mice",
abstract = "The present study reports the effect of ethambutol (EMB) on testicular function. Prepubertal and adult male Swiss albino mice were treated with 40, 80, 160 mg/kg body weight of EMB, intraperitoneally, every alternate day for 4 weeks. After 2 weeks gap, mice were sacrificed to collect caudal spermatozoa. EMB treatment resulted in a dose-dependent decrease in the testicular weight, sperm count and motility while the percentage of sperm with head abnormalities, immature chromatin (P < 0.001) and DNA damage increased (P < 0.01). In addition, EMB treatment resulted in significant depletion of glutathione (P < 0.05–P < 0.01) and histopathological abnormalities such as large cells, vacuolation of tubules and isolated colonies of spermatogenic cells were observed. Oct4, 17β-Hsd and c-Kit mRNA was marginally elevated in EMB treated testes at the highest dose studied. In conclusion, the result of the present study indicates that EMB has adverse effect on testicular function and impairs the sperm functional competence.",
author = "Arpitha Rao and Guruprasad Nayak and Sandhya Kumari and Prabhu, {Amratha D.} and Nalini Khandige and Kalthur, {Sneha Guruprasad} and Srinivas Mutalik and Guruprasad Kalthur and Adiga, {Satish Kumar}",
year = "2016",
month = "10",
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doi = "10.1016/j.etap.2016.08.012",
language = "English",
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pages = "28--37",
journal = "Environmental Toxicology and Pharmacology",
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T1 - Ethambutol induces testicular damage and decreases the sperm functional competence in Swiss albino mice

AU - Rao, Arpitha

AU - Nayak, Guruprasad

AU - Kumari, Sandhya

AU - Prabhu, Amratha D.

AU - Khandige, Nalini

AU - Kalthur, Sneha Guruprasad

AU - Mutalik, Srinivas

AU - Kalthur, Guruprasad

AU - Adiga, Satish Kumar

PY - 2016/10/1

Y1 - 2016/10/1

N2 - The present study reports the effect of ethambutol (EMB) on testicular function. Prepubertal and adult male Swiss albino mice were treated with 40, 80, 160 mg/kg body weight of EMB, intraperitoneally, every alternate day for 4 weeks. After 2 weeks gap, mice were sacrificed to collect caudal spermatozoa. EMB treatment resulted in a dose-dependent decrease in the testicular weight, sperm count and motility while the percentage of sperm with head abnormalities, immature chromatin (P < 0.001) and DNA damage increased (P < 0.01). In addition, EMB treatment resulted in significant depletion of glutathione (P < 0.05–P < 0.01) and histopathological abnormalities such as large cells, vacuolation of tubules and isolated colonies of spermatogenic cells were observed. Oct4, 17β-Hsd and c-Kit mRNA was marginally elevated in EMB treated testes at the highest dose studied. In conclusion, the result of the present study indicates that EMB has adverse effect on testicular function and impairs the sperm functional competence.

AB - The present study reports the effect of ethambutol (EMB) on testicular function. Prepubertal and adult male Swiss albino mice were treated with 40, 80, 160 mg/kg body weight of EMB, intraperitoneally, every alternate day for 4 weeks. After 2 weeks gap, mice were sacrificed to collect caudal spermatozoa. EMB treatment resulted in a dose-dependent decrease in the testicular weight, sperm count and motility while the percentage of sperm with head abnormalities, immature chromatin (P < 0.001) and DNA damage increased (P < 0.01). In addition, EMB treatment resulted in significant depletion of glutathione (P < 0.05–P < 0.01) and histopathological abnormalities such as large cells, vacuolation of tubules and isolated colonies of spermatogenic cells were observed. Oct4, 17β-Hsd and c-Kit mRNA was marginally elevated in EMB treated testes at the highest dose studied. In conclusion, the result of the present study indicates that EMB has adverse effect on testicular function and impairs the sperm functional competence.

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