Ethanolic extract of Moringa oleifera Lam. leaves protect the pre-pubertal spermatogonial cells from cyclophosphamide-induced damage

Guruprasad Nayak, Sachin D. Honguntikar, Sneha Guruprasad Kalthur, Antony Sylvan D'Souza, Srinivas Mutalik, Manjunath M. Setty, Raksha Kalyankumar, Hanumanthappa Krishnamurthy, Guruprasad Kalthur, Satish Kumar Adiga

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ethnopharmacological relevance Moringa oleifera Lam. is widely cultivated in Asian and African countries for its medicinal and dietary significance. The leaves are highly nutritious and are known to possess various biological activities. Materials and methods Pre-pubertal Swiss albino male mice were injected with single dose of cyclophosphamide (CP, 200 mg/kg body weight) or ethanolic extract of Moringa oleifera leaves (MOE, 100 mg/kg body weight) intraperitoneally. In combination group, MOE was administered 24 h prior to CP injection. Results CP induced a significant decrease in testicular weight (p<0.01) and depletion of germ cells (p<0.001) and higher level of DNA damage (p<0.001) compared to control. The expression of P53, Bax, Cytochrome C (Cyt C) was increased while there was a decrease in the expression of Bcl2, c-Kit and Oct4. Administration of MOE 24 h prior to CP treatment ameliorated the depletion (p<0.001), DNA damage (p<0.001) and apoptosis (p<0.01) of germ cells induced by CP. The mitigating effect of MOE appears to be mediated by up-regulating the expression of c-Kit and Oct4 transcripts in P53-independent manner. Conclusion MOE protects the spermatogonial cells from CP-induced damage by modulating the apoptotic response elicited by CP and therefore can be considered as an efficient method of male fertility preservation.

Original languageEnglish
Pages (from-to)101-109
Number of pages9
JournalJournal of Ethnopharmacology
Volume182
DOIs
Publication statusPublished - 22-04-2016

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Moringa oleifera
Germ Cells
Cyclophosphamide
DNA Damage
Body Weight
Fertility Preservation
Apoptosis
Cytochromes
Weights and Measures
Injections
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

@article{a8bb7d81374c402cad2e64ac043376df,
title = "Ethanolic extract of Moringa oleifera Lam. leaves protect the pre-pubertal spermatogonial cells from cyclophosphamide-induced damage",
abstract = "Ethnopharmacological relevance Moringa oleifera Lam. is widely cultivated in Asian and African countries for its medicinal and dietary significance. The leaves are highly nutritious and are known to possess various biological activities. Materials and methods Pre-pubertal Swiss albino male mice were injected with single dose of cyclophosphamide (CP, 200 mg/kg body weight) or ethanolic extract of Moringa oleifera leaves (MOE, 100 mg/kg body weight) intraperitoneally. In combination group, MOE was administered 24 h prior to CP injection. Results CP induced a significant decrease in testicular weight (p<0.01) and depletion of germ cells (p<0.001) and higher level of DNA damage (p<0.001) compared to control. The expression of P53, Bax, Cytochrome C (Cyt C) was increased while there was a decrease in the expression of Bcl2, c-Kit and Oct4. Administration of MOE 24 h prior to CP treatment ameliorated the depletion (p<0.001), DNA damage (p<0.001) and apoptosis (p<0.01) of germ cells induced by CP. The mitigating effect of MOE appears to be mediated by up-regulating the expression of c-Kit and Oct4 transcripts in P53-independent manner. Conclusion MOE protects the spermatogonial cells from CP-induced damage by modulating the apoptotic response elicited by CP and therefore can be considered as an efficient method of male fertility preservation.",
author = "Guruprasad Nayak and Honguntikar, {Sachin D.} and Kalthur, {Sneha Guruprasad} and D'Souza, {Antony Sylvan} and Srinivas Mutalik and Setty, {Manjunath M.} and Raksha Kalyankumar and Hanumanthappa Krishnamurthy and Guruprasad Kalthur and Adiga, {Satish Kumar}",
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Ethanolic extract of Moringa oleifera Lam. leaves protect the pre-pubertal spermatogonial cells from cyclophosphamide-induced damage. / Nayak, Guruprasad; Honguntikar, Sachin D.; Kalthur, Sneha Guruprasad; D'Souza, Antony Sylvan; Mutalik, Srinivas; Setty, Manjunath M.; Kalyankumar, Raksha; Krishnamurthy, Hanumanthappa; Kalthur, Guruprasad; Adiga, Satish Kumar.

In: Journal of Ethnopharmacology, Vol. 182, 22.04.2016, p. 101-109.

Research output: Contribution to journalArticle

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T1 - Ethanolic extract of Moringa oleifera Lam. leaves protect the pre-pubertal spermatogonial cells from cyclophosphamide-induced damage

AU - Nayak, Guruprasad

AU - Honguntikar, Sachin D.

AU - Kalthur, Sneha Guruprasad

AU - D'Souza, Antony Sylvan

AU - Mutalik, Srinivas

AU - Setty, Manjunath M.

AU - Kalyankumar, Raksha

AU - Krishnamurthy, Hanumanthappa

AU - Kalthur, Guruprasad

AU - Adiga, Satish Kumar

PY - 2016/4/22

Y1 - 2016/4/22

N2 - Ethnopharmacological relevance Moringa oleifera Lam. is widely cultivated in Asian and African countries for its medicinal and dietary significance. The leaves are highly nutritious and are known to possess various biological activities. Materials and methods Pre-pubertal Swiss albino male mice were injected with single dose of cyclophosphamide (CP, 200 mg/kg body weight) or ethanolic extract of Moringa oleifera leaves (MOE, 100 mg/kg body weight) intraperitoneally. In combination group, MOE was administered 24 h prior to CP injection. Results CP induced a significant decrease in testicular weight (p<0.01) and depletion of germ cells (p<0.001) and higher level of DNA damage (p<0.001) compared to control. The expression of P53, Bax, Cytochrome C (Cyt C) was increased while there was a decrease in the expression of Bcl2, c-Kit and Oct4. Administration of MOE 24 h prior to CP treatment ameliorated the depletion (p<0.001), DNA damage (p<0.001) and apoptosis (p<0.01) of germ cells induced by CP. The mitigating effect of MOE appears to be mediated by up-regulating the expression of c-Kit and Oct4 transcripts in P53-independent manner. Conclusion MOE protects the spermatogonial cells from CP-induced damage by modulating the apoptotic response elicited by CP and therefore can be considered as an efficient method of male fertility preservation.

AB - Ethnopharmacological relevance Moringa oleifera Lam. is widely cultivated in Asian and African countries for its medicinal and dietary significance. The leaves are highly nutritious and are known to possess various biological activities. Materials and methods Pre-pubertal Swiss albino male mice were injected with single dose of cyclophosphamide (CP, 200 mg/kg body weight) or ethanolic extract of Moringa oleifera leaves (MOE, 100 mg/kg body weight) intraperitoneally. In combination group, MOE was administered 24 h prior to CP injection. Results CP induced a significant decrease in testicular weight (p<0.01) and depletion of germ cells (p<0.001) and higher level of DNA damage (p<0.001) compared to control. The expression of P53, Bax, Cytochrome C (Cyt C) was increased while there was a decrease in the expression of Bcl2, c-Kit and Oct4. Administration of MOE 24 h prior to CP treatment ameliorated the depletion (p<0.001), DNA damage (p<0.001) and apoptosis (p<0.01) of germ cells induced by CP. The mitigating effect of MOE appears to be mediated by up-regulating the expression of c-Kit and Oct4 transcripts in P53-independent manner. Conclusion MOE protects the spermatogonial cells from CP-induced damage by modulating the apoptotic response elicited by CP and therefore can be considered as an efficient method of male fertility preservation.

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