Ethnomedical value of Cissampelos pareira extract in experimentally induced diarrhoea

Amresh [Unknown], G.D. Reddy, C.V. Rao, A. Shirwaikar

Research output: Contribution to journalArticle

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Abstract

The antidiarrhoeal activity of the ethanolic extract of Cis-sampelos pareira (Menispermaceae) roots was assessed on experimental animals. The hydroethanolic extract (25-100 mg dry extract kg-1 body mass, p.o.) exhibited a dose dependent decrease in the total number of faecal droppings (control 65, reduced to 26-46) and 29.2-60.0% inhibition in castor oil-induced diarrhoea. Further, C. pareira produced a significant (p < 0.01) and dose dependent reduction in intestinal fluids accumulation (26.0-59.0%). The extract showed a greater inhibitory effect on the concentration of Na+ (20.0 and 34.5%) than on the concentration of K+ (6.7 and 9.4%). The extract also reduced dose dependently the gastrointestinal transit from 46.4 and 38.7%, equivalent to 53.6 and 61.3%. However, C. pareira significantly reduced the lipid peroxidation and inhibited the decrease in antioxidant enzyme levels (superoxide dismutase and catalase) on prior administration to castor oil-induced fluid accumulation. The extract of C. pareira had no effect on normal defecation at 25 mg kg-1 in mice. However, 50 and 100 mg kg-1 inhibited defecation by 100% in the initial 2 h and the activity was reduced to 40.0 and 73.0%, respectively, in the third hour.
Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalActa Pharmaceutica
Volume54
Issue number1
Publication statusPublished - 2004

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Cissampelos
Castor Oil
Defecation
Diarrhea
Menispermaceae
Gastrointestinal Transit
Antidiarrheals
Catalase
Lipid Peroxidation
Superoxide Dismutase
Antioxidants
Enzymes

Cite this

[Unknown], A., Reddy, G. D., Rao, C. V., & Shirwaikar, A. (2004). Ethnomedical value of Cissampelos pareira extract in experimentally induced diarrhoea. Acta Pharmaceutica, 54(1), 27-35.
[Unknown], Amresh ; Reddy, G.D. ; Rao, C.V. ; Shirwaikar, A. / Ethnomedical value of Cissampelos pareira extract in experimentally induced diarrhoea. In: Acta Pharmaceutica. 2004 ; Vol. 54, No. 1. pp. 27-35.
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abstract = "The antidiarrhoeal activity of the ethanolic extract of Cis-sampelos pareira (Menispermaceae) roots was assessed on experimental animals. The hydroethanolic extract (25-100 mg dry extract kg-1 body mass, p.o.) exhibited a dose dependent decrease in the total number of faecal droppings (control 65, reduced to 26-46) and 29.2-60.0{\%} inhibition in castor oil-induced diarrhoea. Further, C. pareira produced a significant (p < 0.01) and dose dependent reduction in intestinal fluids accumulation (26.0-59.0{\%}). The extract showed a greater inhibitory effect on the concentration of Na+ (20.0 and 34.5{\%}) than on the concentration of K+ (6.7 and 9.4{\%}). The extract also reduced dose dependently the gastrointestinal transit from 46.4 and 38.7{\%}, equivalent to 53.6 and 61.3{\%}. However, C. pareira significantly reduced the lipid peroxidation and inhibited the decrease in antioxidant enzyme levels (superoxide dismutase and catalase) on prior administration to castor oil-induced fluid accumulation. The extract of C. pareira had no effect on normal defecation at 25 mg kg-1 in mice. However, 50 and 100 mg kg-1 inhibited defecation by 100{\%} in the initial 2 h and the activity was reduced to 40.0 and 73.0{\%}, respectively, in the third hour.",
author = "Amresh [Unknown] and G.D. Reddy and C.V. Rao and A. Shirwaikar",
note = "Cited By :34 Export Date: 10 November 2017 CODEN: ACPHE Correspondence Address: Rao, C.V.; Ethnopharmacology Division, Natl. Botanical Research Institute, Cncl. of Sci. and Indust. Research, Rana Pratap Marg, Lucknow-226 001, Uttar Pradesh, India; email: chvrao72@yahoo.com Chemicals/CAS: castor oil, 8001-79-4; catalase, 9001-05-2; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Castor Oil, 8001-79-4; Catalase, EC 1.11.1.6; Cathartics; Free Radicals; Peroxidases, EC 1.11.1.-; Plant Extracts; Superoxide Dismutase, EC 1.15.1.1 References: Bapalal Vaidya, G., (1998) Nighantu Adarsa, 2nd Ed., 1, p. 35. , Chaukhambha Bharti Academy Publications, Varanasi; (1992) Wealth of India: Raw Materials, Vol. 3, 3rd Ed., 3, pp. 591-593. , Council of Scientific and Industrial Research Publication, New Delhi; Kirtikar, K.R., Basu, B.D., (1933) Indian Medicinal Plants, 1, p. 96. , Lalit Mohan Basu, Allahabad; Jain, S.K., (1991) Dictionary of Indian Folk Medicine and Ethnobotany, p. 221. , Deep Publications, New Delhi; Zimmerman, M., Ethical guidelines for investigation of experimental pain in conscious animal (1983) Pain, 16, pp. 109-110; Melo, L., Thomas, G., Mukherjee, R., Antidiarrhoeal activity of bisnordihydrotoxiferine isolated from root bark of Strychonus trinervis (Vell.) Mart. (Longaniaceae) (1988) J. Pharm. Pharmacol., 40, pp. 79-82; Awouters, F., Niemegeers, C.J.E., Lenaerts, F.M., Janssen, P.A.J., Delay of castor oil-induced diarrhoea in rats; a new way to evaluate the prostaglandin synthesis (1978) J. Pharm. Pharmacol., 30, pp. 41-45; Nwodo, O.F.C., Alumanah, E.O., Studies on Abrus precatorious seed. II. Antidiarrhoeal activity (1991) J. Ethnopharmacol., 31, pp. 395-398; Robert, A., Nezamis, J.E., Lancaster, C., Hanchar, A.I., Klepper, M.S., Enteropooling assay: A test for diarrhoea produced by prostaglandins (1976) Prostaglandins, 11, pp. 809-814; Di Carlo, G., Mascolo, N., Izzo, A., Capasso, F., Autore, G., Effect of quercetin on gastrointestinal tract in rats and mice (1994) Phytother. Res., 8, pp. 42-45; Lutterodt, G.D., Inhibition of gastrointestinal release of acetylocholine by quercetine as a possible mode of action of Psidium guajava leaf extracts in the treatment of acute diarrhoeal disease (1989) J. Ethnopharmacol., 25, pp. 235-247; Das, D., Banerjee, R.F., Effect of stress on the antioxidant enzymes and gastric ulceration (1993) Mol. Cell Biochem., 125, pp. 115-125; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal. Biochem., 95, pp. 351-358; Kakkar, P., Das, B., Viswanathan, P.N., A modified spectrometric assay of superoxide dismutase (1984) Indian J. Biochem. Biophys., 21, pp. 130-132; Aebi, H., Catalase (1952) Methods in Enzymatic Analysis, 2nd Ed., 3, p. 673. , (Ed. H. U. Bergmeyer), Acadamie Press, New York; Lorke, D., A new approach to practical acute toxicity testing (1983) Arch. Toxicol., 54, pp. 275-287; Padmanabha Pillai, N., Ramaswamy, S., Gopalakrishinan, V., Ghosh, M.N., Effect of cholinergic drugs on acute and chronic morphine dependence (1982) Arch. Int. Pharmacodyn., 257, pp. 147-154; Sanders, K.M., Evidence that prostaglandins are local regulatory agents in canine ileal circular muscles (1984) Am. J. Physiol., 246, pp. G361-G366; Bruton, L.L., Agent Affecting Gastrointestinal Water Flux and Motility, Digestants and Bile Acids (1985) The Pharmacological Basis of Therapeutics, 2, p. 914. , (Eds. A. G. Gillman, T. W. Rall, A. S. Nies and P. Taylor), 8 th ed., McGraw-Hill, New York; Shoba, F.G., Thomas, M., Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhoea (2001) J. Ethnopharmcol., 76, pp. 73-76; Manonmani, S., William, S., Subramanian, S., Govindaswamy, S., Biochemical studies on the antidiarrhoeal effect of cauvery-100, an ayurvedic formulation in rats (1991) Biochem. Int., 24, pp. 701-708; Sairam, K., Rao, Ch.V., Goel, R.K., Effect of Centella asiatica Linn on physical and chemical factors induced gastric ulceration and secretion in rats (2001) J. Exp. Biol., 39, pp. 137-141; Maxwell, S.R.J., Prospects for the use of antioxidant therapies (1995) Drugs, 49, pp. 345-361; Fridovich, I., Biological effect of superoxide radical (1986) Arch. Biochem. Biophys., 247, pp. 1-11; Halliwell, B., Gutteridge, M.C., Oxygen free radicals and the nervous system (1985) Trends Neurosci., 8, pp. 22-26",
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[Unknown], A, Reddy, GD, Rao, CV & Shirwaikar, A 2004, 'Ethnomedical value of Cissampelos pareira extract in experimentally induced diarrhoea', Acta Pharmaceutica, vol. 54, no. 1, pp. 27-35.

Ethnomedical value of Cissampelos pareira extract in experimentally induced diarrhoea. / [Unknown], Amresh; Reddy, G.D.; Rao, C.V.; Shirwaikar, A.

In: Acta Pharmaceutica, Vol. 54, No. 1, 2004, p. 27-35.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ethnomedical value of Cissampelos pareira extract in experimentally induced diarrhoea

AU - [Unknown], Amresh

AU - Reddy, G.D.

AU - Rao, C.V.

AU - Shirwaikar, A.

N1 - Cited By :34 Export Date: 10 November 2017 CODEN: ACPHE Correspondence Address: Rao, C.V.; Ethnopharmacology Division, Natl. Botanical Research Institute, Cncl. of Sci. and Indust. Research, Rana Pratap Marg, Lucknow-226 001, Uttar Pradesh, India; email: chvrao72@yahoo.com Chemicals/CAS: castor oil, 8001-79-4; catalase, 9001-05-2; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Castor Oil, 8001-79-4; Catalase, EC 1.11.1.6; Cathartics; Free Radicals; Peroxidases, EC 1.11.1.-; Plant Extracts; Superoxide Dismutase, EC 1.15.1.1 References: Bapalal Vaidya, G., (1998) Nighantu Adarsa, 2nd Ed., 1, p. 35. , Chaukhambha Bharti Academy Publications, Varanasi; (1992) Wealth of India: Raw Materials, Vol. 3, 3rd Ed., 3, pp. 591-593. , Council of Scientific and Industrial Research Publication, New Delhi; Kirtikar, K.R., Basu, B.D., (1933) Indian Medicinal Plants, 1, p. 96. , Lalit Mohan Basu, Allahabad; Jain, S.K., (1991) Dictionary of Indian Folk Medicine and Ethnobotany, p. 221. , Deep Publications, New Delhi; Zimmerman, M., Ethical guidelines for investigation of experimental pain in conscious animal (1983) Pain, 16, pp. 109-110; Melo, L., Thomas, G., Mukherjee, R., Antidiarrhoeal activity of bisnordihydrotoxiferine isolated from root bark of Strychonus trinervis (Vell.) Mart. (Longaniaceae) (1988) J. Pharm. Pharmacol., 40, pp. 79-82; Awouters, F., Niemegeers, C.J.E., Lenaerts, F.M., Janssen, P.A.J., Delay of castor oil-induced diarrhoea in rats; a new way to evaluate the prostaglandin synthesis (1978) J. Pharm. Pharmacol., 30, pp. 41-45; Nwodo, O.F.C., Alumanah, E.O., Studies on Abrus precatorious seed. II. Antidiarrhoeal activity (1991) J. Ethnopharmacol., 31, pp. 395-398; Robert, A., Nezamis, J.E., Lancaster, C., Hanchar, A.I., Klepper, M.S., Enteropooling assay: A test for diarrhoea produced by prostaglandins (1976) Prostaglandins, 11, pp. 809-814; Di Carlo, G., Mascolo, N., Izzo, A., Capasso, F., Autore, G., Effect of quercetin on gastrointestinal tract in rats and mice (1994) Phytother. Res., 8, pp. 42-45; Lutterodt, G.D., Inhibition of gastrointestinal release of acetylocholine by quercetine as a possible mode of action of Psidium guajava leaf extracts in the treatment of acute diarrhoeal disease (1989) J. Ethnopharmacol., 25, pp. 235-247; Das, D., Banerjee, R.F., Effect of stress on the antioxidant enzymes and gastric ulceration (1993) Mol. Cell Biochem., 125, pp. 115-125; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal. Biochem., 95, pp. 351-358; Kakkar, P., Das, B., Viswanathan, P.N., A modified spectrometric assay of superoxide dismutase (1984) Indian J. Biochem. Biophys., 21, pp. 130-132; Aebi, H., Catalase (1952) Methods in Enzymatic Analysis, 2nd Ed., 3, p. 673. , (Ed. H. U. Bergmeyer), Acadamie Press, New York; Lorke, D., A new approach to practical acute toxicity testing (1983) Arch. Toxicol., 54, pp. 275-287; Padmanabha Pillai, N., Ramaswamy, S., Gopalakrishinan, V., Ghosh, M.N., Effect of cholinergic drugs on acute and chronic morphine dependence (1982) Arch. Int. Pharmacodyn., 257, pp. 147-154; Sanders, K.M., Evidence that prostaglandins are local regulatory agents in canine ileal circular muscles (1984) Am. J. Physiol., 246, pp. G361-G366; Bruton, L.L., Agent Affecting Gastrointestinal Water Flux and Motility, Digestants and Bile Acids (1985) The Pharmacological Basis of Therapeutics, 2, p. 914. , (Eds. A. G. Gillman, T. W. Rall, A. S. Nies and P. Taylor), 8 th ed., McGraw-Hill, New York; Shoba, F.G., Thomas, M., Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhoea (2001) J. Ethnopharmcol., 76, pp. 73-76; Manonmani, S., William, S., Subramanian, S., Govindaswamy, S., Biochemical studies on the antidiarrhoeal effect of cauvery-100, an ayurvedic formulation in rats (1991) Biochem. Int., 24, pp. 701-708; Sairam, K., Rao, Ch.V., Goel, R.K., Effect of Centella asiatica Linn on physical and chemical factors induced gastric ulceration and secretion in rats (2001) J. Exp. Biol., 39, pp. 137-141; Maxwell, S.R.J., Prospects for the use of antioxidant therapies (1995) Drugs, 49, pp. 345-361; Fridovich, I., Biological effect of superoxide radical (1986) Arch. Biochem. Biophys., 247, pp. 1-11; Halliwell, B., Gutteridge, M.C., Oxygen free radicals and the nervous system (1985) Trends Neurosci., 8, pp. 22-26

PY - 2004

Y1 - 2004

N2 - The antidiarrhoeal activity of the ethanolic extract of Cis-sampelos pareira (Menispermaceae) roots was assessed on experimental animals. The hydroethanolic extract (25-100 mg dry extract kg-1 body mass, p.o.) exhibited a dose dependent decrease in the total number of faecal droppings (control 65, reduced to 26-46) and 29.2-60.0% inhibition in castor oil-induced diarrhoea. Further, C. pareira produced a significant (p < 0.01) and dose dependent reduction in intestinal fluids accumulation (26.0-59.0%). The extract showed a greater inhibitory effect on the concentration of Na+ (20.0 and 34.5%) than on the concentration of K+ (6.7 and 9.4%). The extract also reduced dose dependently the gastrointestinal transit from 46.4 and 38.7%, equivalent to 53.6 and 61.3%. However, C. pareira significantly reduced the lipid peroxidation and inhibited the decrease in antioxidant enzyme levels (superoxide dismutase and catalase) on prior administration to castor oil-induced fluid accumulation. The extract of C. pareira had no effect on normal defecation at 25 mg kg-1 in mice. However, 50 and 100 mg kg-1 inhibited defecation by 100% in the initial 2 h and the activity was reduced to 40.0 and 73.0%, respectively, in the third hour.

AB - The antidiarrhoeal activity of the ethanolic extract of Cis-sampelos pareira (Menispermaceae) roots was assessed on experimental animals. The hydroethanolic extract (25-100 mg dry extract kg-1 body mass, p.o.) exhibited a dose dependent decrease in the total number of faecal droppings (control 65, reduced to 26-46) and 29.2-60.0% inhibition in castor oil-induced diarrhoea. Further, C. pareira produced a significant (p < 0.01) and dose dependent reduction in intestinal fluids accumulation (26.0-59.0%). The extract showed a greater inhibitory effect on the concentration of Na+ (20.0 and 34.5%) than on the concentration of K+ (6.7 and 9.4%). The extract also reduced dose dependently the gastrointestinal transit from 46.4 and 38.7%, equivalent to 53.6 and 61.3%. However, C. pareira significantly reduced the lipid peroxidation and inhibited the decrease in antioxidant enzyme levels (superoxide dismutase and catalase) on prior administration to castor oil-induced fluid accumulation. The extract of C. pareira had no effect on normal defecation at 25 mg kg-1 in mice. However, 50 and 100 mg kg-1 inhibited defecation by 100% in the initial 2 h and the activity was reduced to 40.0 and 73.0%, respectively, in the third hour.

M3 - Article

VL - 54

SP - 27

EP - 35

JO - Acta Pharmaceutica

JF - Acta Pharmaceutica

SN - 1330-0075

IS - 1

ER -