Evaluation of anti-epileptic property of Marsilea quadrifolia Linn. in maximal electroshock and pentylenetetrazole-induced rat models of epilepsy

Adhikari Snehunsu, N. Mukunda, M. C. Satish Kumar, Nandi Sadhana, Sareesh Naduvil Narayanan, K. Vijay Kapgal, H. Avinash, B. R. Chandrashekar, K. Raghavendra Rao, B. Satheesha Nayak

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To study the anti-epileptic effects of methanolic extract of Marsilea quadrifolia Linn. (MQ) in maximal electroshock (MES) and pentylenetetrazole (PTZ) induced rat models of epilepsy. Method: A total of 84 adult male Wistar rats were used. An acute oral toxicity study was conducted on 36 rats and the remaining were used for other experiments. Each model had 24 rats which were allotted into four groups (n=6). Group I (Control) received 1% carboxymethyl cellulose solution, Group II (Positive control) received phenytoin 300mgkg-1 b.w. in the MES model; sodium valproate 200mgkg -1 b.w. in the PTZ model, Group III (MQ) received 400mgkg -1 b.w. MQ extract and Group IV (MQ) received 600mgkg-1 b.w. MQ extract. Hind limb extension (HLE) time and recovery time were noted in the MES model. Latency for myoclonic jerk, seizures and EEG was recorded in the PTZ model. Results: When compared to control, the phenytoin received group did not show HLE. In MQ pre-treated groups only 50% of rats showed HLE. Sodium valproate and various doses of MQ significantly increased the latency for onset of clonus and seizures. PTZ-induced EEG alterations were significantly attenuated by MQ administration and this was comparable to that of the sodium valproate effect. Conclusion: Marsilea quadrifolia Linn. showed significant anti-epileptic efficacy against various epilepsy models.

Original languageEnglish
Pages (from-to)1707-1714
Number of pages8
JournalBrain Injury
Volume27
Issue number13-14
DOIs
Publication statusPublished - 01-01-2013

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Marsileaceae
Electroshock
Pentylenetetrazole
Epilepsy
Valproic Acid
Extremities
Phenytoin
Electroencephalography
Seizures
Carboxymethylcellulose Sodium
Myoclonus
Wistar Rats
Control Groups
Rat
Evaluation

All Science Journal Classification (ASJC) codes

  • Developmental and Educational Psychology
  • Arts and Humanities (miscellaneous)
  • Clinical Neurology

Cite this

Snehunsu, Adhikari ; Mukunda, N. ; Satish Kumar, M. C. ; Sadhana, Nandi ; Naduvil Narayanan, Sareesh ; Vijay Kapgal, K. ; Avinash, H. ; Chandrashekar, B. R. ; Raghavendra Rao, K. ; Nayak, B. Satheesha. / Evaluation of anti-epileptic property of Marsilea quadrifolia Linn. in maximal electroshock and pentylenetetrazole-induced rat models of epilepsy. In: Brain Injury. 2013 ; Vol. 27, No. 13-14. pp. 1707-1714.
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title = "Evaluation of anti-epileptic property of Marsilea quadrifolia Linn. in maximal electroshock and pentylenetetrazole-induced rat models of epilepsy",
abstract = "Objective: To study the anti-epileptic effects of methanolic extract of Marsilea quadrifolia Linn. (MQ) in maximal electroshock (MES) and pentylenetetrazole (PTZ) induced rat models of epilepsy. Method: A total of 84 adult male Wistar rats were used. An acute oral toxicity study was conducted on 36 rats and the remaining were used for other experiments. Each model had 24 rats which were allotted into four groups (n=6). Group I (Control) received 1{\%} carboxymethyl cellulose solution, Group II (Positive control) received phenytoin 300mgkg-1 b.w. in the MES model; sodium valproate 200mgkg -1 b.w. in the PTZ model, Group III (MQ) received 400mgkg -1 b.w. MQ extract and Group IV (MQ) received 600mgkg-1 b.w. MQ extract. Hind limb extension (HLE) time and recovery time were noted in the MES model. Latency for myoclonic jerk, seizures and EEG was recorded in the PTZ model. Results: When compared to control, the phenytoin received group did not show HLE. In MQ pre-treated groups only 50{\%} of rats showed HLE. Sodium valproate and various doses of MQ significantly increased the latency for onset of clonus and seizures. PTZ-induced EEG alterations were significantly attenuated by MQ administration and this was comparable to that of the sodium valproate effect. Conclusion: Marsilea quadrifolia Linn. showed significant anti-epileptic efficacy against various epilepsy models.",
author = "Adhikari Snehunsu and N. Mukunda and {Satish Kumar}, {M. C.} and Nandi Sadhana and {Naduvil Narayanan}, Sareesh and {Vijay Kapgal}, K. and H. Avinash and Chandrashekar, {B. R.} and {Raghavendra Rao}, K. and Nayak, {B. Satheesha}",
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Evaluation of anti-epileptic property of Marsilea quadrifolia Linn. in maximal electroshock and pentylenetetrazole-induced rat models of epilepsy. / Snehunsu, Adhikari; Mukunda, N.; Satish Kumar, M. C.; Sadhana, Nandi; Naduvil Narayanan, Sareesh; Vijay Kapgal, K.; Avinash, H.; Chandrashekar, B. R.; Raghavendra Rao, K.; Nayak, B. Satheesha.

In: Brain Injury, Vol. 27, No. 13-14, 01.01.2013, p. 1707-1714.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of anti-epileptic property of Marsilea quadrifolia Linn. in maximal electroshock and pentylenetetrazole-induced rat models of epilepsy

AU - Snehunsu, Adhikari

AU - Mukunda, N.

AU - Satish Kumar, M. C.

AU - Sadhana, Nandi

AU - Naduvil Narayanan, Sareesh

AU - Vijay Kapgal, K.

AU - Avinash, H.

AU - Chandrashekar, B. R.

AU - Raghavendra Rao, K.

AU - Nayak, B. Satheesha

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Objective: To study the anti-epileptic effects of methanolic extract of Marsilea quadrifolia Linn. (MQ) in maximal electroshock (MES) and pentylenetetrazole (PTZ) induced rat models of epilepsy. Method: A total of 84 adult male Wistar rats were used. An acute oral toxicity study was conducted on 36 rats and the remaining were used for other experiments. Each model had 24 rats which were allotted into four groups (n=6). Group I (Control) received 1% carboxymethyl cellulose solution, Group II (Positive control) received phenytoin 300mgkg-1 b.w. in the MES model; sodium valproate 200mgkg -1 b.w. in the PTZ model, Group III (MQ) received 400mgkg -1 b.w. MQ extract and Group IV (MQ) received 600mgkg-1 b.w. MQ extract. Hind limb extension (HLE) time and recovery time were noted in the MES model. Latency for myoclonic jerk, seizures and EEG was recorded in the PTZ model. Results: When compared to control, the phenytoin received group did not show HLE. In MQ pre-treated groups only 50% of rats showed HLE. Sodium valproate and various doses of MQ significantly increased the latency for onset of clonus and seizures. PTZ-induced EEG alterations were significantly attenuated by MQ administration and this was comparable to that of the sodium valproate effect. Conclusion: Marsilea quadrifolia Linn. showed significant anti-epileptic efficacy against various epilepsy models.

AB - Objective: To study the anti-epileptic effects of methanolic extract of Marsilea quadrifolia Linn. (MQ) in maximal electroshock (MES) and pentylenetetrazole (PTZ) induced rat models of epilepsy. Method: A total of 84 adult male Wistar rats were used. An acute oral toxicity study was conducted on 36 rats and the remaining were used for other experiments. Each model had 24 rats which were allotted into four groups (n=6). Group I (Control) received 1% carboxymethyl cellulose solution, Group II (Positive control) received phenytoin 300mgkg-1 b.w. in the MES model; sodium valproate 200mgkg -1 b.w. in the PTZ model, Group III (MQ) received 400mgkg -1 b.w. MQ extract and Group IV (MQ) received 600mgkg-1 b.w. MQ extract. Hind limb extension (HLE) time and recovery time were noted in the MES model. Latency for myoclonic jerk, seizures and EEG was recorded in the PTZ model. Results: When compared to control, the phenytoin received group did not show HLE. In MQ pre-treated groups only 50% of rats showed HLE. Sodium valproate and various doses of MQ significantly increased the latency for onset of clonus and seizures. PTZ-induced EEG alterations were significantly attenuated by MQ administration and this was comparable to that of the sodium valproate effect. Conclusion: Marsilea quadrifolia Linn. showed significant anti-epileptic efficacy against various epilepsy models.

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DO - 10.3109/02699052.2013.831121

M3 - Article

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EP - 1714

JO - Brain Injury

JF - Brain Injury

SN - 0269-9052

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