Evaluation of antiurolithiatic and antioxidant potential of Lepidagathis prostrata

A Pashanbhed plant

Raviraj Anand Devkar, Shilpee Chaudhary, Sahithi Adepu, Seena Kanniparambil Xavier, Kodangala Subraya Chandrashekar, Manganahalli Manjunath Setty

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Context: Oxidative stress acts as an essential mediator in the pathophysiology of urolithiasis. Lepidagathis prostrata Dalz. (Acanthaceae) is a Pashanbhed plant that is recommended for the management of urolithiasis; however, no scientific validation has been reported. Objectives: To evaluate the antiurolithiatic and antioxidant potential of L. prostrata. Materials and methods: Methanol extract (LPM) and fractions; petroleum ether (LPPE), ethyl acetate (LPEA), n-butanol (LPBU) and aqueous (LPAQ) were prepared. In vitro antiurolithiatic activity was evaluated by the capacity to inhibit calcium oxalate (CaOx) nucleation and aggregation at different concentrations of extract/fractions (0.04–3 mg/mL) for 30 min. Total phenol and flavonoid content and antioxidant potential were determined. A validated HPTLC method was performed to quantify lupeol and β-sitosterol. Results: LPEA exhibited the highest dose-dependent inhibition of CaOx nucleation (IC50: 336.23 ± 30.79 µg/mL) and aggregation (IC50: 149.63 ± 10.31 µg/mL), which was significantly (p < 0.05) better than standard Cystone®. The polar LPBU fraction was enriched with phenols (47.34 ± 0.19 mg GAE/g) and flavonoids (20.38 ± 0.05 mg QE/g), which correlates with its highest antioxidant potential in DPPH, ABTS, nitric oxide scavenging and iron chelating activities (IC50: 1.18–87.34 µg/mL). To our knowledge, this is the first study reporting the presence of lupeol and β-sitosterol in L. prostrata. Conclusion: The antiurolithiatic activity of L. prostrata is probably mediated through the inhibition of CaOx crystallization. In addition to its free radical scavenging and antioxidant activities, it would act as an excellent agent for the prevention of urolithiasis.

Original languageEnglish
Pages (from-to)1237-1245
Number of pages9
JournalPharmaceutical Biology
Volume54
Issue number7
DOIs
Publication statusPublished - 02-07-2016

Fingerprint

Calcium Oxalate
Urolithiasis
Antioxidants
Inhibitory Concentration 50
1-Butanol
Flavonoids
Acanthaceae
Phenols
Crystallization
Phenol
Free Radicals
Methanol
Nitric Oxide
Oxidative Stress
Iron
lupeol
ethyl acetate
gamma-sitosterol

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Devkar, Raviraj Anand ; Chaudhary, Shilpee ; Adepu, Sahithi ; Xavier, Seena Kanniparambil ; Chandrashekar, Kodangala Subraya ; Setty, Manganahalli Manjunath. / Evaluation of antiurolithiatic and antioxidant potential of Lepidagathis prostrata : A Pashanbhed plant. In: Pharmaceutical Biology. 2016 ; Vol. 54, No. 7. pp. 1237-1245.
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abstract = "Context: Oxidative stress acts as an essential mediator in the pathophysiology of urolithiasis. Lepidagathis prostrata Dalz. (Acanthaceae) is a Pashanbhed plant that is recommended for the management of urolithiasis; however, no scientific validation has been reported. Objectives: To evaluate the antiurolithiatic and antioxidant potential of L. prostrata. Materials and methods: Methanol extract (LPM) and fractions; petroleum ether (LPPE), ethyl acetate (LPEA), n-butanol (LPBU) and aqueous (LPAQ) were prepared. In vitro antiurolithiatic activity was evaluated by the capacity to inhibit calcium oxalate (CaOx) nucleation and aggregation at different concentrations of extract/fractions (0.04–3 mg/mL) for 30 min. Total phenol and flavonoid content and antioxidant potential were determined. A validated HPTLC method was performed to quantify lupeol and β-sitosterol. Results: LPEA exhibited the highest dose-dependent inhibition of CaOx nucleation (IC50: 336.23 ± 30.79 µg/mL) and aggregation (IC50: 149.63 ± 10.31 µg/mL), which was significantly (p < 0.05) better than standard Cystone{\circledR}. The polar LPBU fraction was enriched with phenols (47.34 ± 0.19 mg GAE/g) and flavonoids (20.38 ± 0.05 mg QE/g), which correlates with its highest antioxidant potential in DPPH, ABTS, nitric oxide scavenging and iron chelating activities (IC50: 1.18–87.34 µg/mL). To our knowledge, this is the first study reporting the presence of lupeol and β-sitosterol in L. prostrata. Conclusion: The antiurolithiatic activity of L. prostrata is probably mediated through the inhibition of CaOx crystallization. In addition to its free radical scavenging and antioxidant activities, it would act as an excellent agent for the prevention of urolithiasis.",
author = "Devkar, {Raviraj Anand} and Shilpee Chaudhary and Sahithi Adepu and Xavier, {Seena Kanniparambil} and Chandrashekar, {Kodangala Subraya} and Setty, {Manganahalli Manjunath}",
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Evaluation of antiurolithiatic and antioxidant potential of Lepidagathis prostrata : A Pashanbhed plant. / Devkar, Raviraj Anand; Chaudhary, Shilpee; Adepu, Sahithi; Xavier, Seena Kanniparambil; Chandrashekar, Kodangala Subraya; Setty, Manganahalli Manjunath.

In: Pharmaceutical Biology, Vol. 54, No. 7, 02.07.2016, p. 1237-1245.

Research output: Contribution to journalArticle

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T1 - Evaluation of antiurolithiatic and antioxidant potential of Lepidagathis prostrata

T2 - A Pashanbhed plant

AU - Devkar, Raviraj Anand

AU - Chaudhary, Shilpee

AU - Adepu, Sahithi

AU - Xavier, Seena Kanniparambil

AU - Chandrashekar, Kodangala Subraya

AU - Setty, Manganahalli Manjunath

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N2 - Context: Oxidative stress acts as an essential mediator in the pathophysiology of urolithiasis. Lepidagathis prostrata Dalz. (Acanthaceae) is a Pashanbhed plant that is recommended for the management of urolithiasis; however, no scientific validation has been reported. Objectives: To evaluate the antiurolithiatic and antioxidant potential of L. prostrata. Materials and methods: Methanol extract (LPM) and fractions; petroleum ether (LPPE), ethyl acetate (LPEA), n-butanol (LPBU) and aqueous (LPAQ) were prepared. In vitro antiurolithiatic activity was evaluated by the capacity to inhibit calcium oxalate (CaOx) nucleation and aggregation at different concentrations of extract/fractions (0.04–3 mg/mL) for 30 min. Total phenol and flavonoid content and antioxidant potential were determined. A validated HPTLC method was performed to quantify lupeol and β-sitosterol. Results: LPEA exhibited the highest dose-dependent inhibition of CaOx nucleation (IC50: 336.23 ± 30.79 µg/mL) and aggregation (IC50: 149.63 ± 10.31 µg/mL), which was significantly (p < 0.05) better than standard Cystone®. The polar LPBU fraction was enriched with phenols (47.34 ± 0.19 mg GAE/g) and flavonoids (20.38 ± 0.05 mg QE/g), which correlates with its highest antioxidant potential in DPPH, ABTS, nitric oxide scavenging and iron chelating activities (IC50: 1.18–87.34 µg/mL). To our knowledge, this is the first study reporting the presence of lupeol and β-sitosterol in L. prostrata. Conclusion: The antiurolithiatic activity of L. prostrata is probably mediated through the inhibition of CaOx crystallization. In addition to its free radical scavenging and antioxidant activities, it would act as an excellent agent for the prevention of urolithiasis.

AB - Context: Oxidative stress acts as an essential mediator in the pathophysiology of urolithiasis. Lepidagathis prostrata Dalz. (Acanthaceae) is a Pashanbhed plant that is recommended for the management of urolithiasis; however, no scientific validation has been reported. Objectives: To evaluate the antiurolithiatic and antioxidant potential of L. prostrata. Materials and methods: Methanol extract (LPM) and fractions; petroleum ether (LPPE), ethyl acetate (LPEA), n-butanol (LPBU) and aqueous (LPAQ) were prepared. In vitro antiurolithiatic activity was evaluated by the capacity to inhibit calcium oxalate (CaOx) nucleation and aggregation at different concentrations of extract/fractions (0.04–3 mg/mL) for 30 min. Total phenol and flavonoid content and antioxidant potential were determined. A validated HPTLC method was performed to quantify lupeol and β-sitosterol. Results: LPEA exhibited the highest dose-dependent inhibition of CaOx nucleation (IC50: 336.23 ± 30.79 µg/mL) and aggregation (IC50: 149.63 ± 10.31 µg/mL), which was significantly (p < 0.05) better than standard Cystone®. The polar LPBU fraction was enriched with phenols (47.34 ± 0.19 mg GAE/g) and flavonoids (20.38 ± 0.05 mg QE/g), which correlates with its highest antioxidant potential in DPPH, ABTS, nitric oxide scavenging and iron chelating activities (IC50: 1.18–87.34 µg/mL). To our knowledge, this is the first study reporting the presence of lupeol and β-sitosterol in L. prostrata. Conclusion: The antiurolithiatic activity of L. prostrata is probably mediated through the inhibition of CaOx crystallization. In addition to its free radical scavenging and antioxidant activities, it would act as an excellent agent for the prevention of urolithiasis.

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