Evaluation of Culture, Antigen Detection and Polymerase Chain Reaction for Detection of Vaginal Colonization of Group B Streptococcus (GBS) in Pregnant Women

Kavitha Paul Konikkara, Shrikala Baliga, Suchithra Shenoy, B Bharati

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12 Citations (Scopus)

Abstract

BACKGROUND AND OBJECTIVE: Group B Streptococcal infection is an important cause of neonatal morbidity and mortality. Early detection of perinatal vagino-rectal (VR) carriage of Group B Streptococcus (GBS) is important in the management of newborn infections. The objective of the study was to evaluate Culture, antigen detection and Polymerase chain reaction for detection of GBS in Pregnant women.

SETTINGS AND DESIGN: Observational descriptive study was done in a tertiary care hospital in Southern India.

MATERIALS AND METHODS: VR swabs were collected from 50 women at 35 to 37 weeks of gestation. Culture in a selective Lim enrichment broth with subsequent culture on 5% sheep blood agar, Conventional PCR assay and antigen detection method were performed. The performance of antigen detection and PCR methods were compared with culture.

STATISTICAL ANALYSIS: STATISTICAL ANALYSIS was performed by Chi-Square test.

RESULTS: GBS cultures were positive for 16% of the specimen (8 out of 50). Considering culture as a gold standard, Sensitivity, Specificity, Positive predictive value and Negative predictive value of antigen detection was 100%, 92.86%, 72.73%, 100% and similarly for that of PCR was 100%, 45.23%, 25.80%, 100%, respectively.

CONCLUSION: Antigen detection method was the rapid, sensitive and specific test for the detection of GBS colonizers during pregnancy.

Original languageEnglish
Pages (from-to)47-9
Number of pages3
JournalJournal of Clinical and Diagnostic Research
Volume8
Issue number2
DOIs
Publication statusPublished - 02-2014

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Streptococcus agalactiae
Polymerase chain reaction
Pregnant Women
Antigens
Polymerase Chain Reaction
Statistical methods
Pregnancy
Streptococcal Infections
Infant Mortality
Tertiary Healthcare
Chi-Square Distribution
Tertiary Care Centers
Agar
Observational Studies
India
Assays
Sheep
Blood
Newborn Infant
Morbidity

Cite this

@article{7baf6c1c4c3e42ceb14921ae62911a54,
title = "Evaluation of Culture, Antigen Detection and Polymerase Chain Reaction for Detection of Vaginal Colonization of Group B Streptococcus (GBS) in Pregnant Women",
abstract = "BACKGROUND AND OBJECTIVE: Group B Streptococcal infection is an important cause of neonatal morbidity and mortality. Early detection of perinatal vagino-rectal (VR) carriage of Group B Streptococcus (GBS) is important in the management of newborn infections. The objective of the study was to evaluate Culture, antigen detection and Polymerase chain reaction for detection of GBS in Pregnant women.SETTINGS AND DESIGN: Observational descriptive study was done in a tertiary care hospital in Southern India.MATERIALS AND METHODS: VR swabs were collected from 50 women at 35 to 37 weeks of gestation. Culture in a selective Lim enrichment broth with subsequent culture on 5{\%} sheep blood agar, Conventional PCR assay and antigen detection method were performed. The performance of antigen detection and PCR methods were compared with culture.STATISTICAL ANALYSIS: STATISTICAL ANALYSIS was performed by Chi-Square test.RESULTS: GBS cultures were positive for 16{\%} of the specimen (8 out of 50). Considering culture as a gold standard, Sensitivity, Specificity, Positive predictive value and Negative predictive value of antigen detection was 100{\%}, 92.86{\%}, 72.73{\%}, 100{\%} and similarly for that of PCR was 100{\%}, 45.23{\%}, 25.80{\%}, 100{\%}, respectively.CONCLUSION: Antigen detection method was the rapid, sensitive and specific test for the detection of GBS colonizers during pregnancy.",
author = "Konikkara, {Kavitha Paul} and Shrikala Baliga and Suchithra Shenoy and B Bharati",
year = "2014",
month = "2",
doi = "10.7860/JCDR/2014/6675.4004",
language = "English",
volume = "8",
pages = "47--9",
journal = "Journal of Clinical and Diagnostic Research",
issn = "2249-782X",
publisher = "Journal of Clinical and Diagnostic Research",
number = "2",

}

TY - JOUR

T1 - Evaluation of Culture, Antigen Detection and Polymerase Chain Reaction for Detection of Vaginal Colonization of Group B Streptococcus (GBS) in Pregnant Women

AU - Konikkara, Kavitha Paul

AU - Baliga, Shrikala

AU - Shenoy, Suchithra

AU - Bharati, B

PY - 2014/2

Y1 - 2014/2

N2 - BACKGROUND AND OBJECTIVE: Group B Streptococcal infection is an important cause of neonatal morbidity and mortality. Early detection of perinatal vagino-rectal (VR) carriage of Group B Streptococcus (GBS) is important in the management of newborn infections. The objective of the study was to evaluate Culture, antigen detection and Polymerase chain reaction for detection of GBS in Pregnant women.SETTINGS AND DESIGN: Observational descriptive study was done in a tertiary care hospital in Southern India.MATERIALS AND METHODS: VR swabs were collected from 50 women at 35 to 37 weeks of gestation. Culture in a selective Lim enrichment broth with subsequent culture on 5% sheep blood agar, Conventional PCR assay and antigen detection method were performed. The performance of antigen detection and PCR methods were compared with culture.STATISTICAL ANALYSIS: STATISTICAL ANALYSIS was performed by Chi-Square test.RESULTS: GBS cultures were positive for 16% of the specimen (8 out of 50). Considering culture as a gold standard, Sensitivity, Specificity, Positive predictive value and Negative predictive value of antigen detection was 100%, 92.86%, 72.73%, 100% and similarly for that of PCR was 100%, 45.23%, 25.80%, 100%, respectively.CONCLUSION: Antigen detection method was the rapid, sensitive and specific test for the detection of GBS colonizers during pregnancy.

AB - BACKGROUND AND OBJECTIVE: Group B Streptococcal infection is an important cause of neonatal morbidity and mortality. Early detection of perinatal vagino-rectal (VR) carriage of Group B Streptococcus (GBS) is important in the management of newborn infections. The objective of the study was to evaluate Culture, antigen detection and Polymerase chain reaction for detection of GBS in Pregnant women.SETTINGS AND DESIGN: Observational descriptive study was done in a tertiary care hospital in Southern India.MATERIALS AND METHODS: VR swabs were collected from 50 women at 35 to 37 weeks of gestation. Culture in a selective Lim enrichment broth with subsequent culture on 5% sheep blood agar, Conventional PCR assay and antigen detection method were performed. The performance of antigen detection and PCR methods were compared with culture.STATISTICAL ANALYSIS: STATISTICAL ANALYSIS was performed by Chi-Square test.RESULTS: GBS cultures were positive for 16% of the specimen (8 out of 50). Considering culture as a gold standard, Sensitivity, Specificity, Positive predictive value and Negative predictive value of antigen detection was 100%, 92.86%, 72.73%, 100% and similarly for that of PCR was 100%, 45.23%, 25.80%, 100%, respectively.CONCLUSION: Antigen detection method was the rapid, sensitive and specific test for the detection of GBS colonizers during pregnancy.

U2 - 10.7860/JCDR/2014/6675.4004

DO - 10.7860/JCDR/2014/6675.4004

M3 - Article

VL - 8

SP - 47

EP - 49

JO - Journal of Clinical and Diagnostic Research

JF - Journal of Clinical and Diagnostic Research

SN - 2249-782X

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