Evaluation of cytotoxic effects of dichloromethane extract of guduchi (Tinospora cordifolia Miers ex Hook F & Thoms) on cultured HeLa cells

Ganesh Chandra Jagetia, Shaival Kamalaksha Rao

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Extracts of Tinospora cordifolia (TCE) have been shown to possess anti-tumor properties, but the mechanism of the anti-tumor function of TCE is poorly understood. This investigation elucidates the possible mechanism underlying the cytotoxic effects of dichlormethane extracts of TCE, after selecting optimal duration and concentration for treatment. HeLa cells were exposed to various concentrations of TCE, which has resulted in a concentration-dependent decline in the clonogenicity, glutathione-S-transferase (GST) activity and a concentration-dependent increase in lipid peroxidation (TBARS) with a peak at 4 h and lactate dehydrogenase (LDH) release with a peak at 2 h. Our results suggest that the cytotoxic effect of TCE may be due to lipid peroxidation and release of LDH and decline in GST.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalEvidence-based Complementary and Alternative Medicine
Volume3
Issue number2
DOIs
Publication statusPublished - 01-06-2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

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