Evaluation of hepatoprotective activity of combination of Phyllanthus niruri and Curcuma longa extracts in wistar rats

S. Adiga, B. S. Vinay, Shobha Kamath, Bhaskar Gaonkar, Amit Panda, K. G. Mohandas Rao, K. L. Bairy

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Hepatoprotective activity of combination of Phyllanthus niruri(PN) and Curcuma longa(CL) extract was evaluated against carbon tetrachloride(CCl4) induced liver damage. Combination of PN+CL extract at a dose of 400mg/kg, orally was coadministered with CCl4 (0.5 mg/kg i.p) to rats for 7 days. On 8th day serum enzyme levels such as AST, ALT, ALP, TB were determined. Thiopentone induced sleeping time is estimated as an indirect index of functionality of liver. Liver tissue was used to estimate antioxidants such as MDA, GST levels and for histopathological assessment. There was a significant increase in serum enzyme levels and duration of thiopentone induced sleep time in CCl4 treated rats. Coadministration of PN+CL extract combination with CCl4 significantly prevented the rise in serum enzyme levels and normalize the duration of thiopentone induced sleep time. Combination of PN +CL produced significant reduction and increase in MDA & GST liver levels respectively. Histological section of liver in animals treated with CCl4 showed centrilobular area of necrosis with derangement in hepatic architecture. PN+CL administration prevented these deleterious changes, histological section of liver in rats treated with PN+CL showed normal hepatic parenchyma. Combination of PN+CL extract showed significant hepatoprotection against CCl4 induced liver damage.

Original languageEnglish
Pages (from-to)1260-1268
Number of pages9
JournalResearch Journal of Pharmaceutical, Biological and Chemical Sciences
Issue number3
Publication statusPublished - 01-07-2012


All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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